Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Aft...
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ftdoajarticles:oai:doaj.org/article:1870e341ab27427f9e34dc591e435a30 2024-09-09T19:27:03+00:00 Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique NUNES Cáris Maroni TUNDISI Regina Nardini GARCIA José Fernando HEINEMANN Marcos Brayan OGASSAWARA Saemi RICHTZENHAIN Leonardo José 1997-01-01T00:00:00Z https://doaj.org/article/1870e341ab27427f9e34dc591e435a30 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000500002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/1870e341ab27427f9e34dc591e435a30 Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 5, Pp 253-256 (1997) Toxocara canis antigens Immunodiagnosis Visceral larva migrans Ascaris suum Western blotting Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1997 ftdoajarticles 2024-08-05T17:49:30Z Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis e A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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topic |
Toxocara canis antigens Immunodiagnosis Visceral larva migrans Ascaris suum Western blotting Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Toxocara canis antigens Immunodiagnosis Visceral larva migrans Ascaris suum Western blotting Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 NUNES Cáris Maroni TUNDISI Regina Nardini GARCIA José Fernando HEINEMANN Marcos Brayan OGASSAWARA Saemi RICHTZENHAIN Leonardo José Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
topic_facet |
Toxocara canis antigens Immunodiagnosis Visceral larva migrans Ascaris suum Western blotting Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis e A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed |
format |
Article in Journal/Newspaper |
author |
NUNES Cáris Maroni TUNDISI Regina Nardini GARCIA José Fernando HEINEMANN Marcos Brayan OGASSAWARA Saemi RICHTZENHAIN Leonardo José |
author_facet |
NUNES Cáris Maroni TUNDISI Regina Nardini GARCIA José Fernando HEINEMANN Marcos Brayan OGASSAWARA Saemi RICHTZENHAIN Leonardo José |
author_sort |
NUNES Cáris Maroni |
title |
Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_short |
Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_full |
Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_fullStr |
Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_full_unstemmed |
Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_sort |
cross-reactions between toxocara canis and ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1997 |
url |
https://doaj.org/article/1870e341ab27427f9e34dc591e435a30 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 5, Pp 253-256 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000500002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/1870e341ab27427f9e34dc591e435a30 |
_version_ |
1809896556554354688 |