Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostre...
Published in: | Frontiers in Cellular and Infection Microbiology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fcimb.2022.858311 https://doaj.org/article/185ba3935284441bbea1b360672e6567 |
id |
ftdoajarticles:oai:doaj.org/article:185ba3935284441bbea1b360672e6567 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:185ba3935284441bbea1b360672e6567 2023-05-15T15:57:49+02:00 Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection Sandy Picot Nicole Faury Camille Pelletier Isabelle Arzul Bruno Chollet Lionel Dégremont Tristan Renault Benjamin Morga 2022-04-01T00:00:00Z https://doi.org/10.3389/fcimb.2022.858311 https://doaj.org/article/185ba3935284441bbea1b360672e6567 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fcimb.2022.858311/full https://doaj.org/toc/2235-2988 2235-2988 doi:10.3389/fcimb.2022.858311 https://doaj.org/article/185ba3935284441bbea1b360672e6567 Frontiers in Cellular and Infection Microbiology, Vol 12 (2022) autophagy Pacific oyster (Crassostrea gigas) herpesvirus innate immunity invertebrate Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3389/fcimb.2022.858311 2022-12-31T04:28:32Z Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Cellular and Infection Microbiology 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
autophagy Pacific oyster (Crassostrea gigas) herpesvirus innate immunity invertebrate Microbiology QR1-502 |
spellingShingle |
autophagy Pacific oyster (Crassostrea gigas) herpesvirus innate immunity invertebrate Microbiology QR1-502 Sandy Picot Nicole Faury Camille Pelletier Isabelle Arzul Bruno Chollet Lionel Dégremont Tristan Renault Benjamin Morga Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
topic_facet |
autophagy Pacific oyster (Crassostrea gigas) herpesvirus innate immunity invertebrate Microbiology QR1-502 |
description |
Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. |
format |
Article in Journal/Newspaper |
author |
Sandy Picot Nicole Faury Camille Pelletier Isabelle Arzul Bruno Chollet Lionel Dégremont Tristan Renault Benjamin Morga |
author_facet |
Sandy Picot Nicole Faury Camille Pelletier Isabelle Arzul Bruno Chollet Lionel Dégremont Tristan Renault Benjamin Morga |
author_sort |
Sandy Picot |
title |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_short |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_fullStr |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full_unstemmed |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_sort |
monitoring autophagy at cellular and molecular level in crassostrea gigas during an experimental ostreid herpesvirus 1 (oshv-1) infection |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fcimb.2022.858311 https://doaj.org/article/185ba3935284441bbea1b360672e6567 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Cellular and Infection Microbiology, Vol 12 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fcimb.2022.858311/full https://doaj.org/toc/2235-2988 2235-2988 doi:10.3389/fcimb.2022.858311 https://doaj.org/article/185ba3935284441bbea1b360672e6567 |
op_doi |
https://doi.org/10.3389/fcimb.2022.858311 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
_version_ |
1766393522007572480 |