Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum
Abstract Background Plasmodium falciparum chloroquine resistance (CQR) transporter protein (PfCRT) is known to be the important key of CQR. Recent studies have definitively demonstrated a link between mutations in the gene pfcrt and resistance to chloroquine in P. falciparum . Although these mutatio...
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ftdoajarticles:oai:doaj.org/article:17cd3e588d804848963a4fb52675d7b9 2023-05-15T15:08:43+02:00 Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum Bray Patrick G Owen Andrew Johnson David Mungthin Mathirut Ward Stephen A Chaijaroenkul Wanna Na-Bangchang Kesara 2011-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-42 https://doaj.org/article/17cd3e588d804848963a4fb52675d7b9 EN eng BMC http://www.malariajournal.com/content/10/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-42 1475-2875 https://doaj.org/article/17cd3e588d804848963a4fb52675d7b9 Malaria Journal, Vol 10, Iss 1, p 42 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-42 2022-12-31T08:43:00Z Abstract Background Plasmodium falciparum chloroquine resistance (CQR) transporter protein (PfCRT) is known to be the important key of CQR. Recent studies have definitively demonstrated a link between mutations in the gene pfcrt and resistance to chloroquine in P. falciparum . Although these mutations are predictive of chloroquine resistance, they are not quantitatively predictive of the degree of resistance. Methods In this study, a total of 95 recently adapted P. falciparum isolates from Thailand were included in the analysis. Parasites were characterized for their drug susceptibility phenotypes and genotypes with respect to pfcrt . From the original 95 isolates, 20 were selected for complete pfcrt sequence analysis. Results Almost all of the parasites characterized carried the previously reported mutations K76T, A220S, Q271E, N326S, I356T and R371I. On complete sequencing, isolates were identified with novel mutations at K76A and E198K. There was a suggestion that parasites carrying E198K were less resistant than those that did not. In addition, pfcrt and pfmdr1 gene expression were investigated by real-time PCR. No relationship between the expression level of either of these genes and response to drug was observed. Conclusion Data from the present study suggest that other genes must contribute to the degree of resistance once the resistance phenotype is established through mutations in pfcrt . Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 42 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Bray Patrick G Owen Andrew Johnson David Mungthin Mathirut Ward Stephen A Chaijaroenkul Wanna Na-Bangchang Kesara Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum chloroquine resistance (CQR) transporter protein (PfCRT) is known to be the important key of CQR. Recent studies have definitively demonstrated a link between mutations in the gene pfcrt and resistance to chloroquine in P. falciparum . Although these mutations are predictive of chloroquine resistance, they are not quantitatively predictive of the degree of resistance. Methods In this study, a total of 95 recently adapted P. falciparum isolates from Thailand were included in the analysis. Parasites were characterized for their drug susceptibility phenotypes and genotypes with respect to pfcrt . From the original 95 isolates, 20 were selected for complete pfcrt sequence analysis. Results Almost all of the parasites characterized carried the previously reported mutations K76T, A220S, Q271E, N326S, I356T and R371I. On complete sequencing, isolates were identified with novel mutations at K76A and E198K. There was a suggestion that parasites carrying E198K were less resistant than those that did not. In addition, pfcrt and pfmdr1 gene expression were investigated by real-time PCR. No relationship between the expression level of either of these genes and response to drug was observed. Conclusion Data from the present study suggest that other genes must contribute to the degree of resistance once the resistance phenotype is established through mutations in pfcrt . |
format |
Article in Journal/Newspaper |
author |
Bray Patrick G Owen Andrew Johnson David Mungthin Mathirut Ward Stephen A Chaijaroenkul Wanna Na-Bangchang Kesara |
author_facet |
Bray Patrick G Owen Andrew Johnson David Mungthin Mathirut Ward Stephen A Chaijaroenkul Wanna Na-Bangchang Kesara |
author_sort |
Bray Patrick G |
title |
Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
title_short |
Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
title_full |
Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
title_fullStr |
Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
title_full_unstemmed |
Sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of Plasmodium falciparum |
title_sort |
sequence and gene expression of chloroquine resistance transporter ( pfcrt ) in the association of in vitro drugs resistance of plasmodium falciparum |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-42 https://doaj.org/article/17cd3e588d804848963a4fb52675d7b9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 42 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-42 1475-2875 https://doaj.org/article/17cd3e588d804848963a4fb52675d7b9 |
op_doi |
https://doi.org/10.1186/1475-2875-10-42 |
container_title |
Malaria Journal |
container_volume |
10 |
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1 |
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42 |
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1766340035577118720 |