Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system.
We have previously shown that the cell morphogenesis NlpD lipoprotein is essential for virulence of the plague bacteria, Yersinia pestis. To elucidate the role of NlpD in Y. pestis pathogenicity, we conducted a whole-genome comparative transcriptome analysis of the wild-type Y. pestis strain and an...
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ftdoajarticles:oai:doaj.org/article:17a76f7d294c44cf8fcf625af8aca773 2023-05-15T15:07:51+02:00 Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. Avital Tidhar Yinon Levy Ayelet Zauberman Yaron Vagima David Gur Moshe Aftalion Ofir Israeli Theodor Chitlaru Naomi Ariel Yehuda Flashner Anat Zvi Emanuelle Mamroud 2019-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007449 https://doaj.org/article/17a76f7d294c44cf8fcf625af8aca773 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007449 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007449 https://doaj.org/article/17a76f7d294c44cf8fcf625af8aca773 PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007449 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007449 2022-12-31T05:04:46Z We have previously shown that the cell morphogenesis NlpD lipoprotein is essential for virulence of the plague bacteria, Yersinia pestis. To elucidate the role of NlpD in Y. pestis pathogenicity, we conducted a whole-genome comparative transcriptome analysis of the wild-type Y. pestis strain and an nlpD mutant under conditions mimicking early stages of infection. The analysis suggested that NlpD is involved in three phenomena: (i) Envelope stability/integrity evidenced by compensatory up-regulation of the Cpx and Psp membrane stress-response systems in the mutant; (ii) iron acquisition, supported by modulation of iron metabolism genes and by limited growth in iron-deprived medium; (iii) activity of the twin-arginine (Tat) system, which translocates folded proteins across the cytoplasmic membrane. Virulence studies of Y. pestis strains mutated in individual Tat components clearly indicated that the Tat system is central in Y. pestis pathogenicity and substantiated the assumption that NlpD essentiality in iron utilization involves the activity of the Tat system. This study reveals a new role for NlpD in Tat system activity and iron assimilation suggesting a modality by which this lipoprotein is involved in Y. pestis pathogenesis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 6 e0007449 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Avital Tidhar Yinon Levy Ayelet Zauberman Yaron Vagima David Gur Moshe Aftalion Ofir Israeli Theodor Chitlaru Naomi Ariel Yehuda Flashner Anat Zvi Emanuelle Mamroud Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
We have previously shown that the cell morphogenesis NlpD lipoprotein is essential for virulence of the plague bacteria, Yersinia pestis. To elucidate the role of NlpD in Y. pestis pathogenicity, we conducted a whole-genome comparative transcriptome analysis of the wild-type Y. pestis strain and an nlpD mutant under conditions mimicking early stages of infection. The analysis suggested that NlpD is involved in three phenomena: (i) Envelope stability/integrity evidenced by compensatory up-regulation of the Cpx and Psp membrane stress-response systems in the mutant; (ii) iron acquisition, supported by modulation of iron metabolism genes and by limited growth in iron-deprived medium; (iii) activity of the twin-arginine (Tat) system, which translocates folded proteins across the cytoplasmic membrane. Virulence studies of Y. pestis strains mutated in individual Tat components clearly indicated that the Tat system is central in Y. pestis pathogenicity and substantiated the assumption that NlpD essentiality in iron utilization involves the activity of the Tat system. This study reveals a new role for NlpD in Tat system activity and iron assimilation suggesting a modality by which this lipoprotein is involved in Y. pestis pathogenesis. |
format |
Article in Journal/Newspaper |
author |
Avital Tidhar Yinon Levy Ayelet Zauberman Yaron Vagima David Gur Moshe Aftalion Ofir Israeli Theodor Chitlaru Naomi Ariel Yehuda Flashner Anat Zvi Emanuelle Mamroud |
author_facet |
Avital Tidhar Yinon Levy Ayelet Zauberman Yaron Vagima David Gur Moshe Aftalion Ofir Israeli Theodor Chitlaru Naomi Ariel Yehuda Flashner Anat Zvi Emanuelle Mamroud |
author_sort |
Avital Tidhar |
title |
Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
title_short |
Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
title_full |
Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
title_fullStr |
Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
title_full_unstemmed |
Disruption of the NlpD lipoprotein of the plague pathogen Yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
title_sort |
disruption of the nlpd lipoprotein of the plague pathogen yersinia pestis affects iron acquisition and the activity of the twin-arginine translocation system. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007449 https://doaj.org/article/17a76f7d294c44cf8fcf625af8aca773 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007449 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007449 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007449 https://doaj.org/article/17a76f7d294c44cf8fcf625af8aca773 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007449 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e0007449 |
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1766339270311673856 |