Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors.
Background Although infection with Trypanosoma cruzi is thought to be lifelong, less than half of those infected develop cardiomyopathy, suggesting greater parasite control or even clearance. Antibody levels appear to correlate with T. cruzi (antigen) load. We test the association between a downward...
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ftdoajarticles:oai:doaj.org/article:179ce94c9e7b4773a74da1cceaaa54bf 2023-05-15T15:10:47+02:00 Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. Lewis F Buss Léa Campos de Oliveira-da Silva Carlos H V Moreira Erika R Manuli Flavia C Sales Ingra Morales Clara Di Germanio Cesar de Almeida-Neto Sonia Bakkour Paul Constable Marcelo M Pinto-Filho Antonio L Ribeiro Michael Busch Ester C Sabino 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008787 https://doaj.org/article/179ce94c9e7b4773a74da1cceaaa54bf EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008787 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008787 https://doaj.org/article/179ce94c9e7b4773a74da1cceaaa54bf PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008787 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008787 2022-12-31T07:50:09Z Background Although infection with Trypanosoma cruzi is thought to be lifelong, less than half of those infected develop cardiomyopathy, suggesting greater parasite control or even clearance. Antibody levels appear to correlate with T. cruzi (antigen) load. We test the association between a downwards antibody trajectory, PCR positivity and ECG alterations in untreated individuals with Chagas disease. Methodology/principal findings This is a retrospective cohort of T. cruzi seropositive blood donors. Paired blood samples (index donation and follow-up) were tested using the VITROS Immunodiagnostic Products Anti-T.cruzi (Chagas) assay (Ortho Clinical Diagnostics, Raritan NJ) and PCR performed on the follow-up sample. A 12-lead resting ECG was performed. Significant antibody decline was defined as a reduction of > 1 signal-to-cutoff (S/CO) unit on the VITROS assay. Follow-up S/CO of < 4 was defined as borderline/low. 276 untreated seropositive blood donors were included. The median (IQR) follow-up was 12.7 years (8.5-16.9). 56 (22.1%) subjects had a significant antibody decline and 35 (12.7%) had a low/borderline follow-up result. PCR positivity was lower in the falling (26.8% vs 52.8%, p = 0.001) and low/borderline (17.1% vs 51.9%, p < 0.001) antibody groups, as was the rate of ECG abnormalities. Falling and low/borderline antibody groups were predominantly composed of individuals with negative PCR and normal ECG findings: 64% and 71%, respectively. Conclusions/significance Low and falling antibody levels define a phenotype of possible spontaneous parasite clearance. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008787 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lewis F Buss Léa Campos de Oliveira-da Silva Carlos H V Moreira Erika R Manuli Flavia C Sales Ingra Morales Clara Di Germanio Cesar de Almeida-Neto Sonia Bakkour Paul Constable Marcelo M Pinto-Filho Antonio L Ribeiro Michael Busch Ester C Sabino Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Although infection with Trypanosoma cruzi is thought to be lifelong, less than half of those infected develop cardiomyopathy, suggesting greater parasite control or even clearance. Antibody levels appear to correlate with T. cruzi (antigen) load. We test the association between a downwards antibody trajectory, PCR positivity and ECG alterations in untreated individuals with Chagas disease. Methodology/principal findings This is a retrospective cohort of T. cruzi seropositive blood donors. Paired blood samples (index donation and follow-up) were tested using the VITROS Immunodiagnostic Products Anti-T.cruzi (Chagas) assay (Ortho Clinical Diagnostics, Raritan NJ) and PCR performed on the follow-up sample. A 12-lead resting ECG was performed. Significant antibody decline was defined as a reduction of > 1 signal-to-cutoff (S/CO) unit on the VITROS assay. Follow-up S/CO of < 4 was defined as borderline/low. 276 untreated seropositive blood donors were included. The median (IQR) follow-up was 12.7 years (8.5-16.9). 56 (22.1%) subjects had a significant antibody decline and 35 (12.7%) had a low/borderline follow-up result. PCR positivity was lower in the falling (26.8% vs 52.8%, p = 0.001) and low/borderline (17.1% vs 51.9%, p < 0.001) antibody groups, as was the rate of ECG abnormalities. Falling and low/borderline antibody groups were predominantly composed of individuals with negative PCR and normal ECG findings: 64% and 71%, respectively. Conclusions/significance Low and falling antibody levels define a phenotype of possible spontaneous parasite clearance. |
format |
Article in Journal/Newspaper |
author |
Lewis F Buss Léa Campos de Oliveira-da Silva Carlos H V Moreira Erika R Manuli Flavia C Sales Ingra Morales Clara Di Germanio Cesar de Almeida-Neto Sonia Bakkour Paul Constable Marcelo M Pinto-Filho Antonio L Ribeiro Michael Busch Ester C Sabino |
author_facet |
Lewis F Buss Léa Campos de Oliveira-da Silva Carlos H V Moreira Erika R Manuli Flavia C Sales Ingra Morales Clara Di Germanio Cesar de Almeida-Neto Sonia Bakkour Paul Constable Marcelo M Pinto-Filho Antonio L Ribeiro Michael Busch Ester C Sabino |
author_sort |
Lewis F Buss |
title |
Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
title_short |
Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
title_full |
Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
title_fullStr |
Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
title_full_unstemmed |
Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors. |
title_sort |
declining antibody levels to trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated brazilian blood donors. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008787 https://doaj.org/article/179ce94c9e7b4773a74da1cceaaa54bf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008787 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008787 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008787 https://doaj.org/article/179ce94c9e7b4773a74da1cceaaa54bf |
op_doi |
https://doi.org/10.1371/journal.pntd.0008787 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0008787 |
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1766341738209738752 |