Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25
Objective: To define the effect of PM2.5 exposure on NF- κ B signaling pathway and airway inflammation, and to reveal the role of NF- K B signaling pathway in airway inflammation induced by atmospheric PM2.5 in rats. Air pollution condition plays an important role in inflammatory response process. P...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:17942d78439a4e19af985932acdb151c 2023-05-15T15:18:10+02:00 Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 Ru-xuan Zhang Na Li Jin Jin Ting-ting Jiang Yu-wei Wu Li-ting Zhou 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.243108 https://doaj.org/article/17942d78439a4e19af985932acdb151c EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=13;spage=40;epage=40;aulast=Zhang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.243108 https://doaj.org/article/17942d78439a4e19af985932acdb151c Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 13, Pp 40-40 (2018) NF-κB PM2.5 airway inflammation I κ B-α Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.243108 2022-12-31T10:19:15Z Objective: To define the effect of PM2.5 exposure on NF- κ B signaling pathway and airway inflammation, and to reveal the role of NF- K B signaling pathway in airway inflammation induced by atmospheric PM2.5 in rats. Air pollution condition plays an important role in inflammatory response process. Previous studies had suggested that atmospheric PM2.5 was a common environmental problem, influencing almost 98% Southeast Asian populations. Therefore, to study the effect of atmospheric PM2.5 on airway inflammation and its mechanism is of great significance. Methods: Atmospheric PM2.5 from urban traffic trunk roads was collected and prepared into the dry powder. Twenty-four wistar rats (half male and half female) were divided into four groups. Rats in low concentration, middle concentration and high concentration groups were given PM2.5 saline suspension of respectively 1.5, 7.5, 37.5 mg/kg by intratracheal instillation, while rats in control group were given saline. HE stain was used to observe the inflammation reaction of the airway tissue. The levels of TNF-α and IL-1β in rats’ bronchoalveolar lavage fluid (BALF) and serum were measured with ELISA. The expression level of IκB-α protein in the airway of rats was detected by western blot. SPSS 17.0 was used for statistical analysis. Results: The organ coefficient of trachea in the group receiving 1.5 mg/kg PM2.5 was significantly higher than that in control group. In group receiving 7.5 mg/kg PM2.5, the lung tissue inflammation was obvious and the structure damage was mild, while in group of 37.5 mg/kg PM2.5, the airway inflammation was mild and the structure damage was severe. In the serum of rats exposed to the 1.5 mg/kg PM2.5, the level of inflammatory factor TNF-a was significantly lower than that in the control group. The level of TNF-a in BALF of rats exposed to PM2.5 was higher than the control group, and with the exposure dose increasing, the level has decreased. The expression of IκB-α protein in lung tissue of rats exposed to the 1.5 mg/kg PM2.5 has ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 13 40 |
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topic |
NF-κB PM2.5 airway inflammation I κ B-α Arctic medicine. Tropical medicine RC955-962 |
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NF-κB PM2.5 airway inflammation I κ B-α Arctic medicine. Tropical medicine RC955-962 Ru-xuan Zhang Na Li Jin Jin Ting-ting Jiang Yu-wei Wu Li-ting Zhou Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
topic_facet |
NF-κB PM2.5 airway inflammation I κ B-α Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To define the effect of PM2.5 exposure on NF- κ B signaling pathway and airway inflammation, and to reveal the role of NF- K B signaling pathway in airway inflammation induced by atmospheric PM2.5 in rats. Air pollution condition plays an important role in inflammatory response process. Previous studies had suggested that atmospheric PM2.5 was a common environmental problem, influencing almost 98% Southeast Asian populations. Therefore, to study the effect of atmospheric PM2.5 on airway inflammation and its mechanism is of great significance. Methods: Atmospheric PM2.5 from urban traffic trunk roads was collected and prepared into the dry powder. Twenty-four wistar rats (half male and half female) were divided into four groups. Rats in low concentration, middle concentration and high concentration groups were given PM2.5 saline suspension of respectively 1.5, 7.5, 37.5 mg/kg by intratracheal instillation, while rats in control group were given saline. HE stain was used to observe the inflammation reaction of the airway tissue. The levels of TNF-α and IL-1β in rats’ bronchoalveolar lavage fluid (BALF) and serum were measured with ELISA. The expression level of IκB-α protein in the airway of rats was detected by western blot. SPSS 17.0 was used for statistical analysis. Results: The organ coefficient of trachea in the group receiving 1.5 mg/kg PM2.5 was significantly higher than that in control group. In group receiving 7.5 mg/kg PM2.5, the lung tissue inflammation was obvious and the structure damage was mild, while in group of 37.5 mg/kg PM2.5, the airway inflammation was mild and the structure damage was severe. In the serum of rats exposed to the 1.5 mg/kg PM2.5, the level of inflammatory factor TNF-a was significantly lower than that in the control group. The level of TNF-a in BALF of rats exposed to PM2.5 was higher than the control group, and with the exposure dose increasing, the level has decreased. The expression of IκB-α protein in lung tissue of rats exposed to the 1.5 mg/kg PM2.5 has ... |
format |
Article in Journal/Newspaper |
author |
Ru-xuan Zhang Na Li Jin Jin Ting-ting Jiang Yu-wei Wu Li-ting Zhou |
author_facet |
Ru-xuan Zhang Na Li Jin Jin Ting-ting Jiang Yu-wei Wu Li-ting Zhou |
author_sort |
Ru-xuan Zhang |
title |
Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
title_short |
Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
title_full |
Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
title_fullStr |
Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
title_full_unstemmed |
Role of NF- κ B signaling pathway in airway inflammation of rats induced by atmospheric PM25 |
title_sort |
role of nf- κ b signaling pathway in airway inflammation of rats induced by atmospheric pm25 |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.243108 https://doaj.org/article/17942d78439a4e19af985932acdb151c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 13, Pp 40-40 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=13;spage=40;epage=40;aulast=Zhang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.243108 https://doaj.org/article/17942d78439a4e19af985932acdb151c |
op_doi |
https://doi.org/10.4103/1995-7645.243108 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
13 |
container_start_page |
40 |
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1766348403940261888 |