Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
Abstract Background: L-Glutamate (L-Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when L-Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary...
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ftdoajarticles:oai:doaj.org/article:1782240150854fb193dfc1b33014f80e 2023-05-15T15:17:38+02:00 Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures Eduardo Octaviano Primini José Luiz Liberato Andreia Cristina Karklin Fontana Wagner Ferreira dos Santos 2019-05-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-1488-18 https://doaj.org/article/1782240150854fb193dfc1b33014f80e EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100311&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-1488-18 https://doaj.org/article/1782240150854fb193dfc1b33014f80e Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Iss 0 (2019) L-Glutamate excitotoxicity neuroprotection Parawixia bistriata RT10 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2019 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-1488-18 2022-12-31T02:41:27Z Abstract Background: L-Glutamate (L-Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when L-Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary damage implicated in many neurological disorders. Current therapies for the treatment of neurological disorders are ineffective and have side effects associated with their use; therefore, there is a need to develop novel treatments. In this regard, previous studies have shown that neuroactive compounds obtained from the venom of the spider Parawixia bistriata have neuroprotective effects in vitro and in vivo. In this sense, this work aimed to evaluate potential neuroprotective effects of fraction RT10, obtained from this spider venom, on primary cultures of neuron and glial cells subjected to glutamate excitotoxicity insults. Methods: Primary cultures of neurons and glia were obtained from the cerebral tissue of 1-day-old postnatal Wistar rats. After 7 days in vitro (DIV), the cultures were incubated with fraction RT10 (0.002; 0.02; 0.2 and 2 µg/µL) or riluzole (100 µM) for 3-hours before application of 5 mM L-Glu. After 12 hours, the resazurin sodium salt (RSS) test was applied to measure metabolic activity and proliferation of living cells, whereas immunocytochemistry for MAP2 was performed to measure neuronal survival. In addition, the cells were immunolabeled with NeuN and GFAP in baseline conditions. Results: In the RSS tests, we observed that pre-incubation with RT10 before the excitotoxic insults from L-Glu resulted in neuroprotection, shown by a 10% reduction in the cell death level. RT10 was more effective than riluzole, which resulted in a cell-death reduction of 5%. Moreover, qualitative analysis of neuronal morphology (by MAP2 staining, expressed as fluorescence intensity (FI), an indirect measure of neuronal survival) indicate that RT10 reduced the toxic effects of L-Glu, as shown by a 38 % increase in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 25 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
L-Glutamate excitotoxicity neuroprotection Parawixia bistriata RT10 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
L-Glutamate excitotoxicity neuroprotection Parawixia bistriata RT10 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Eduardo Octaviano Primini José Luiz Liberato Andreia Cristina Karklin Fontana Wagner Ferreira dos Santos Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
topic_facet |
L-Glutamate excitotoxicity neuroprotection Parawixia bistriata RT10 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: L-Glutamate (L-Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when L-Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary damage implicated in many neurological disorders. Current therapies for the treatment of neurological disorders are ineffective and have side effects associated with their use; therefore, there is a need to develop novel treatments. In this regard, previous studies have shown that neuroactive compounds obtained from the venom of the spider Parawixia bistriata have neuroprotective effects in vitro and in vivo. In this sense, this work aimed to evaluate potential neuroprotective effects of fraction RT10, obtained from this spider venom, on primary cultures of neuron and glial cells subjected to glutamate excitotoxicity insults. Methods: Primary cultures of neurons and glia were obtained from the cerebral tissue of 1-day-old postnatal Wistar rats. After 7 days in vitro (DIV), the cultures were incubated with fraction RT10 (0.002; 0.02; 0.2 and 2 µg/µL) or riluzole (100 µM) for 3-hours before application of 5 mM L-Glu. After 12 hours, the resazurin sodium salt (RSS) test was applied to measure metabolic activity and proliferation of living cells, whereas immunocytochemistry for MAP2 was performed to measure neuronal survival. In addition, the cells were immunolabeled with NeuN and GFAP in baseline conditions. Results: In the RSS tests, we observed that pre-incubation with RT10 before the excitotoxic insults from L-Glu resulted in neuroprotection, shown by a 10% reduction in the cell death level. RT10 was more effective than riluzole, which resulted in a cell-death reduction of 5%. Moreover, qualitative analysis of neuronal morphology (by MAP2 staining, expressed as fluorescence intensity (FI), an indirect measure of neuronal survival) indicate that RT10 reduced the toxic effects of L-Glu, as shown by a 38 % increase in ... |
format |
Article in Journal/Newspaper |
author |
Eduardo Octaviano Primini José Luiz Liberato Andreia Cristina Karklin Fontana Wagner Ferreira dos Santos |
author_facet |
Eduardo Octaviano Primini José Luiz Liberato Andreia Cristina Karklin Fontana Wagner Ferreira dos Santos |
author_sort |
Eduardo Octaviano Primini |
title |
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
title_short |
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
title_full |
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
title_fullStr |
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
title_full_unstemmed |
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
title_sort |
neuroprotective properties of rt10, a fraction isolated from parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures |
publisher |
SciELO |
publishDate |
2019 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-1488-18 https://doaj.org/article/1782240150854fb193dfc1b33014f80e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Iss 0 (2019) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100311&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-1488-18 https://doaj.org/article/1782240150854fb193dfc1b33014f80e |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-1488-18 |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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25 |
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1766347866930937856 |