Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques.
BACKGROUND:Zika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because of the evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome in adults...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005168 https://doaj.org/article/175ab2ad3bf54d07b63e407ea32a0dfb |
id |
ftdoajarticles:oai:doaj.org/article:175ab2ad3bf54d07b63e407ea32a0dfb |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:175ab2ad3bf54d07b63e407ea32a0dfb 2023-05-15T15:13:31+02:00 Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. Matthew T Aliota Dawn M Dudley Christina M Newman Emma L Mohr Dane D Gellerup Meghan E Breitbach Connor R Buechler Mustafa N Rasheed Mariel S Mohns Andrea M Weiler Gabrielle L Barry Kim L Weisgrau Josh A Eudailey Eva G Rakasz Logan J Vosler Jennifer Post Saverio Capuano Thaddeus G Golos Sallie R Permar Jorge E Osorio Thomas C Friedrich Shelby L O'Connor David H O'Connor 2016-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005168 https://doaj.org/article/175ab2ad3bf54d07b63e407ea32a0dfb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5135040?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005168 https://doaj.org/article/175ab2ad3bf54d07b63e407ea32a0dfb PLoS Neglected Tropical Diseases, Vol 10, Iss 12, p e0005168 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005168 2022-12-31T11:50:59Z BACKGROUND:Zika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because of the evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome in adults and congenital birth defects including microcephaly in the developing fetus. Because development of a ZIKV vaccine is a top research priority and because the genetic and antigenic variability of many RNA viruses limits the effectiveness of vaccines, assessing whether immunity elicited against one ZIKV strain is sufficient to confer broad protection against all ZIKV strains is critical. Recently, in vitro studies demonstrated that ZIKV likely circulates as a single serotype. Here, we demonstrate that immunity elicited by African lineage ZIKV protects rhesus macaques against subsequent infection with Asian lineage ZIKV. METHODOLOGY/PRINCIPAL FINDINGS:Using our recently developed rhesus macaque model of ZIKV infection, we report that the prototypical ZIKV strain MR766 productively infects macaques, and that immunity elicited by MR766 protects macaques against heterologous Asian ZIKV. Furthermore, using next generation deep sequencing, we found in vivo restoration of a putative N-linked glycosylation site upon replication in macaques that is absent in numerous MR766 strains that are widely being used by the research community. This reversion highlights the importance of carefully examining the sequence composition of all viral stocks as well as understanding how passage history may alter a virus from its original form. CONCLUSIONS/SIGNIFICANCE:An effective ZIKV vaccine is needed to prevent infection-associated fetal abnormalities. Macaques whose immune responses were primed by infection with East African ZIKV were completely protected from detectable viremia when subsequently rechallenged with heterologous Asian ZIKV. Therefore, these data suggest that immunogen selection is unlikely to adversely affect the breadth of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 12 e0005168 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Matthew T Aliota Dawn M Dudley Christina M Newman Emma L Mohr Dane D Gellerup Meghan E Breitbach Connor R Buechler Mustafa N Rasheed Mariel S Mohns Andrea M Weiler Gabrielle L Barry Kim L Weisgrau Josh A Eudailey Eva G Rakasz Logan J Vosler Jennifer Post Saverio Capuano Thaddeus G Golos Sallie R Permar Jorge E Osorio Thomas C Friedrich Shelby L O'Connor David H O'Connor Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Zika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because of the evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome in adults and congenital birth defects including microcephaly in the developing fetus. Because development of a ZIKV vaccine is a top research priority and because the genetic and antigenic variability of many RNA viruses limits the effectiveness of vaccines, assessing whether immunity elicited against one ZIKV strain is sufficient to confer broad protection against all ZIKV strains is critical. Recently, in vitro studies demonstrated that ZIKV likely circulates as a single serotype. Here, we demonstrate that immunity elicited by African lineage ZIKV protects rhesus macaques against subsequent infection with Asian lineage ZIKV. METHODOLOGY/PRINCIPAL FINDINGS:Using our recently developed rhesus macaque model of ZIKV infection, we report that the prototypical ZIKV strain MR766 productively infects macaques, and that immunity elicited by MR766 protects macaques against heterologous Asian ZIKV. Furthermore, using next generation deep sequencing, we found in vivo restoration of a putative N-linked glycosylation site upon replication in macaques that is absent in numerous MR766 strains that are widely being used by the research community. This reversion highlights the importance of carefully examining the sequence composition of all viral stocks as well as understanding how passage history may alter a virus from its original form. CONCLUSIONS/SIGNIFICANCE:An effective ZIKV vaccine is needed to prevent infection-associated fetal abnormalities. Macaques whose immune responses were primed by infection with East African ZIKV were completely protected from detectable viremia when subsequently rechallenged with heterologous Asian ZIKV. Therefore, these data suggest that immunogen selection is unlikely to adversely affect the breadth of ... |
format |
Article in Journal/Newspaper |
author |
Matthew T Aliota Dawn M Dudley Christina M Newman Emma L Mohr Dane D Gellerup Meghan E Breitbach Connor R Buechler Mustafa N Rasheed Mariel S Mohns Andrea M Weiler Gabrielle L Barry Kim L Weisgrau Josh A Eudailey Eva G Rakasz Logan J Vosler Jennifer Post Saverio Capuano Thaddeus G Golos Sallie R Permar Jorge E Osorio Thomas C Friedrich Shelby L O'Connor David H O'Connor |
author_facet |
Matthew T Aliota Dawn M Dudley Christina M Newman Emma L Mohr Dane D Gellerup Meghan E Breitbach Connor R Buechler Mustafa N Rasheed Mariel S Mohns Andrea M Weiler Gabrielle L Barry Kim L Weisgrau Josh A Eudailey Eva G Rakasz Logan J Vosler Jennifer Post Saverio Capuano Thaddeus G Golos Sallie R Permar Jorge E Osorio Thomas C Friedrich Shelby L O'Connor David H O'Connor |
author_sort |
Matthew T Aliota |
title |
Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
title_short |
Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
title_full |
Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
title_fullStr |
Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
title_full_unstemmed |
Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. |
title_sort |
heterologous protection against asian zika virus challenge in rhesus macaques. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005168 https://doaj.org/article/175ab2ad3bf54d07b63e407ea32a0dfb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 12, p e0005168 (2016) |
op_relation |
http://europepmc.org/articles/PMC5135040?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005168 https://doaj.org/article/175ab2ad3bf54d07b63e407ea32a0dfb |
op_doi |
https://doi.org/10.1371/journal.pntd.0005168 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
12 |
container_start_page |
e0005168 |
_version_ |
1766344061192503296 |