Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction
Cell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:174868b622304c18b509a1f1b47e38f7 2023-05-15T15:09:28+02:00 Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction Fecchio CJ Dias-Melicio LA Soares AMVC Guimarães S Peraçoli MTS Sartori A 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000300012 https://doaj.org/article/174868b622304c18b509a1f1b47e38f7 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300012 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000300012 1678-9199 https://doaj.org/article/174868b622304c18b509a1f1b47e38f7 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 3, Pp 318-324 (2011) Paracoccidioides brasiliensis cell-free antigens delayed-type hypersensitivity reaction gp43 murine paracoccidioidomycosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000300012 2022-12-31T03:46:24Z Cell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate cellular immunity by the footpad test, in experimental murine paracoccidioidomycosis. Male mice infected with P. brasiliensis 265 strain were challenged in the footpad with CFAg obtained from four- (4d CFAg) or 11-day-old cultures (11d CFAg). The increase in footpad swelling provoked by 4d CFAg and 11d CFAg was similar and showed significant difference in relation to control groups. However, the infiltrate pattern was strikingly different: 4d CFAg induced a predominant mononuclear infiltrate whereas 11d CFAg provoked a predominant polymophonuclear infiltrate. These different inflammatory patterns were associated with distinct electrophoretic characteristics. By comparison with 11d CFAg, 4d CFAg showed more numerous and intense bands, including a strong one of 43 kDa (gp43). These results suggest that CFAg derived from Pb 265 isolate can be used as a reagent to evaluate cellular immunity; however, the culture's age is critical because only young cultures are able to induce a typical mononuclear infiltrate. The efficacy of this new paracoccidioidin to assay the cellular immunity in infections caused by other P. brasiliensis isolates is under investigation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 3 318 324 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Paracoccidioides brasiliensis cell-free antigens delayed-type hypersensitivity reaction gp43 murine paracoccidioidomycosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Paracoccidioides brasiliensis cell-free antigens delayed-type hypersensitivity reaction gp43 murine paracoccidioidomycosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Fecchio CJ Dias-Melicio LA Soares AMVC Guimarães S Peraçoli MTS Sartori A Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
topic_facet |
Paracoccidioides brasiliensis cell-free antigens delayed-type hypersensitivity reaction gp43 murine paracoccidioidomycosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Cell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate cellular immunity by the footpad test, in experimental murine paracoccidioidomycosis. Male mice infected with P. brasiliensis 265 strain were challenged in the footpad with CFAg obtained from four- (4d CFAg) or 11-day-old cultures (11d CFAg). The increase in footpad swelling provoked by 4d CFAg and 11d CFAg was similar and showed significant difference in relation to control groups. However, the infiltrate pattern was strikingly different: 4d CFAg induced a predominant mononuclear infiltrate whereas 11d CFAg provoked a predominant polymophonuclear infiltrate. These different inflammatory patterns were associated with distinct electrophoretic characteristics. By comparison with 11d CFAg, 4d CFAg showed more numerous and intense bands, including a strong one of 43 kDa (gp43). These results suggest that CFAg derived from Pb 265 isolate can be used as a reagent to evaluate cellular immunity; however, the culture's age is critical because only young cultures are able to induce a typical mononuclear infiltrate. The efficacy of this new paracoccidioidin to assay the cellular immunity in infections caused by other P. brasiliensis isolates is under investigation. |
format |
Article in Journal/Newspaper |
author |
Fecchio CJ Dias-Melicio LA Soares AMVC Guimarães S Peraçoli MTS Sartori A |
author_facet |
Fecchio CJ Dias-Melicio LA Soares AMVC Guimarães S Peraçoli MTS Sartori A |
author_sort |
Fecchio CJ |
title |
Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
title_short |
Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
title_full |
Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
title_fullStr |
Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
title_full_unstemmed |
Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
title_sort |
cell-free antigens from precocious paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000300012 https://doaj.org/article/174868b622304c18b509a1f1b47e38f7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 3, Pp 318-324 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300012 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000300012 1678-9199 https://doaj.org/article/174868b622304c18b509a1f1b47e38f7 |
op_doi |
https://doi.org/10.1590/S1678-91992011000300012 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
3 |
container_start_page |
318 |
op_container_end_page |
324 |
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1766340657916411904 |