Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws.
Yaws, caused by Treponema pallidum ssp. pertenue, is a neglected tropical disease closely related to venereal syphilis and is targeted for eradication by 2020. Latent yaws represents a diagnostic challenge, and current tools cannot adequately distinguish between individuals with true latent infectio...
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ftdoajarticles:oai:doaj.org/article:16e2859f669b48b6a8cb8282be8f61bd 2023-05-15T15:12:01+02:00 Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. Michael Marks Samantha Katz Kai-Hua Chi Ventis Vahi Yongcheng Sun David C Mabey Anthony W Solomon Cheng Y Chen Allan Pillay 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003905 https://doaj.org/article/16e2859f669b48b6a8cb8282be8f61bd EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4488379?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003905 https://doaj.org/article/16e2859f669b48b6a8cb8282be8f61bd PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003905 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003905 2022-12-31T03:00:43Z Yaws, caused by Treponema pallidum ssp. pertenue, is a neglected tropical disease closely related to venereal syphilis and is targeted for eradication by 2020. Latent yaws represents a diagnostic challenge, and current tools cannot adequately distinguish between individuals with true latent infection and individuals who are serofast following successful treatment. PCR on blood has previously been shown to detect T. pallidum DNA in patients with syphilis, suggesting that this approach may be of value in yaws. We performed real-time PCR for Treponema pallidum ssp. pertenue on blood samples from 140 children with positive T. pallidum Particle Agglutination (TPPA) and Rapid Plasma Reagin (RPR) tests and 7 controls (negative serology), all collected as part of a prospective study of yaws in the Solomon Islands. All samples were also tested by a nested PCR for T. pallidum. 12 patients had clinical evidence of active yaws whilst 128 were considered to have latent yaws. 43 children had high titre rapid plasma reagins (RPRs) of ≥1:32. PCR testing with both assays gave negative results in all cases. It is possible that the failure to detect T. pallidum ssp. pertenue in blood reflects lower loads of organism in latent yaws compared to those in latent infection with T. pallidum ssp. pertenue, and/or a lower propensity for haematogenous dissemination in yaws than in syphilis. As the goal of the yaws control programme is eradication, a tool that can differentiate true latent infection from individuals who are serofast would be of value; however, PCR of blood is not that tool. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003905 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Michael Marks Samantha Katz Kai-Hua Chi Ventis Vahi Yongcheng Sun David C Mabey Anthony W Solomon Cheng Y Chen Allan Pillay Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Yaws, caused by Treponema pallidum ssp. pertenue, is a neglected tropical disease closely related to venereal syphilis and is targeted for eradication by 2020. Latent yaws represents a diagnostic challenge, and current tools cannot adequately distinguish between individuals with true latent infection and individuals who are serofast following successful treatment. PCR on blood has previously been shown to detect T. pallidum DNA in patients with syphilis, suggesting that this approach may be of value in yaws. We performed real-time PCR for Treponema pallidum ssp. pertenue on blood samples from 140 children with positive T. pallidum Particle Agglutination (TPPA) and Rapid Plasma Reagin (RPR) tests and 7 controls (negative serology), all collected as part of a prospective study of yaws in the Solomon Islands. All samples were also tested by a nested PCR for T. pallidum. 12 patients had clinical evidence of active yaws whilst 128 were considered to have latent yaws. 43 children had high titre rapid plasma reagins (RPRs) of ≥1:32. PCR testing with both assays gave negative results in all cases. It is possible that the failure to detect T. pallidum ssp. pertenue in blood reflects lower loads of organism in latent yaws compared to those in latent infection with T. pallidum ssp. pertenue, and/or a lower propensity for haematogenous dissemination in yaws than in syphilis. As the goal of the yaws control programme is eradication, a tool that can differentiate true latent infection from individuals who are serofast would be of value; however, PCR of blood is not that tool. |
format |
Article in Journal/Newspaper |
author |
Michael Marks Samantha Katz Kai-Hua Chi Ventis Vahi Yongcheng Sun David C Mabey Anthony W Solomon Cheng Y Chen Allan Pillay |
author_facet |
Michael Marks Samantha Katz Kai-Hua Chi Ventis Vahi Yongcheng Sun David C Mabey Anthony W Solomon Cheng Y Chen Allan Pillay |
author_sort |
Michael Marks |
title |
Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
title_short |
Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
title_full |
Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
title_fullStr |
Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
title_full_unstemmed |
Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws. |
title_sort |
failure of pcr to detect treponema pallidum ssp. pertenue dna in blood in latent yaws. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003905 https://doaj.org/article/16e2859f669b48b6a8cb8282be8f61bd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003905 (2015) |
op_relation |
http://europepmc.org/articles/PMC4488379?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003905 https://doaj.org/article/16e2859f669b48b6a8cb8282be8f61bd |
op_doi |
https://doi.org/10.1371/journal.pntd.0003905 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
6 |
container_start_page |
e0003905 |
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1766342778563854336 |