Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis
We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania c...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1996
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651996000300003 https://doaj.org/article/16e20b6d0b4e4d68a81d34acd0fc8f7f |
id |
ftdoajarticles:oai:doaj.org/article:16e20b6d0b4e4d68a81d34acd0fc8f7f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:16e20b6d0b4e4d68a81d34acd0fc8f7f 2024-09-09T19:28:28+00:00 Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis Ana de Cássia Vexenat Jaime M. Santana Antonio R.L. Teixeira 1996-06-01T00:00:00Z https://doi.org/10.1590/S0036-46651996000300003 https://doaj.org/article/16e20b6d0b4e4d68a81d34acd0fc8f7f EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000300003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000300003 0036-4665 1678-9946 https://doaj.org/article/16e20b6d0b4e4d68a81d34acd0fc8f7f Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 3, Pp 177-185 (1996) Kinetoplastid protozoa Trypanosoma cruzi Leishmania chagasi Leishmania (Viannia) braziliensis Cross-reactive antibodies Human infections Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1996 ftdoajarticles https://doi.org/10.1590/S0036-46651996000300003 2024-08-05T17:49:31Z We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100% positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100% positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95% of Kala-azar and 35% of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15% of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here. Foram detectados anticorpos, nos soros de pacientes com doença de Chagas, Calazar e Leishnnaniose cutâneo-mucosa, que se ligam a antígenos compartilhados pelos três agentes causais. Os pacientes chagásicos mostraram 98 a 100% de soropositividade pelo ELISA quando antígenos de Leishmania braziliensis e de Leishmania chagasi foram usados, respectivamente. Os soros de Calazar mostraram resultados positivos com antígenos de Trypanosoma cruzi ou ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Azar ENVELOPE(-63.733,-63.733,-64.983,-64.983) Revista do Instituto de Medicina Tropical de São Paulo 38 3 177 185 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Kinetoplastid protozoa Trypanosoma cruzi Leishmania chagasi Leishmania (Viannia) braziliensis Cross-reactive antibodies Human infections Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Kinetoplastid protozoa Trypanosoma cruzi Leishmania chagasi Leishmania (Viannia) braziliensis Cross-reactive antibodies Human infections Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ana de Cássia Vexenat Jaime M. Santana Antonio R.L. Teixeira Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
topic_facet |
Kinetoplastid protozoa Trypanosoma cruzi Leishmania chagasi Leishmania (Viannia) braziliensis Cross-reactive antibodies Human infections Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100% positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100% positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95% of Kala-azar and 35% of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15% of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here. Foram detectados anticorpos, nos soros de pacientes com doença de Chagas, Calazar e Leishnnaniose cutâneo-mucosa, que se ligam a antígenos compartilhados pelos três agentes causais. Os pacientes chagásicos mostraram 98 a 100% de soropositividade pelo ELISA quando antígenos de Leishmania braziliensis e de Leishmania chagasi foram usados, respectivamente. Os soros de Calazar mostraram resultados positivos com antígenos de Trypanosoma cruzi ou ... |
format |
Article in Journal/Newspaper |
author |
Ana de Cássia Vexenat Jaime M. Santana Antonio R.L. Teixeira |
author_facet |
Ana de Cássia Vexenat Jaime M. Santana Antonio R.L. Teixeira |
author_sort |
Ana de Cássia Vexenat |
title |
Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
title_short |
Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
title_full |
Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
title_fullStr |
Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
title_full_unstemmed |
Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis Reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários Trypanosoma cruzi, Leishmania chagasi e Leishmania (Viannia) braziliensis |
title_sort |
cross-reactivity of antibodies in human infections by the kinetoplastid protozoa trypanosoma cruzi, leishmania chagasi and leishmania (viannia) braziliensis reatividade cruzada de anticorpos em pacientes com infecções pelos protozoários trypanosoma cruzi, leishmania chagasi e leishmania (viannia) braziliensis |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1996 |
url |
https://doi.org/10.1590/S0036-46651996000300003 https://doaj.org/article/16e20b6d0b4e4d68a81d34acd0fc8f7f |
long_lat |
ENVELOPE(-63.733,-63.733,-64.983,-64.983) |
geographic |
Arctic Azar |
geographic_facet |
Arctic Azar |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 3, Pp 177-185 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000300003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000300003 0036-4665 1678-9946 https://doaj.org/article/16e20b6d0b4e4d68a81d34acd0fc8f7f |
op_doi |
https://doi.org/10.1590/S0036-46651996000300003 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
38 |
container_issue |
3 |
container_start_page |
177 |
op_container_end_page |
185 |
_version_ |
1809897798788710400 |