Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies).
Leptospirosis is a potentially life-threatening but curable zoonosis whose prognosis depends on accurate and timely diagnosis. Because of its non-specific clinical presentation, laboratory testing is essential to confirm the diagnosis. Here, we aimed to assess the performance of two enzyme-linked im...
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ftdoajarticles:oai:doaj.org/article:16c47021dffc4952aece15aae9e37e52 2023-05-15T15:14:36+02:00 Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). Christophe Courdurie Yohann Le Govic Pascale Bourhy Dorothee Alexer Karine Pailla Rafaelle Theodose Raymond Cesaire Jacques Rosine Patrick Hochedez Claude Olive 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005678 https://doaj.org/article/16c47021dffc4952aece15aae9e37e52 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5500375?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005678 https://doaj.org/article/16c47021dffc4952aece15aae9e37e52 PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005678 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005678 2022-12-31T03:54:41Z Leptospirosis is a potentially life-threatening but curable zoonosis whose prognosis depends on accurate and timely diagnosis. Because of its non-specific clinical presentation, laboratory testing is essential to confirm the diagnosis. Here, we aimed to assess the performance of two enzyme-linked immunosorbent assays (ELISAs) (ELISA Serion and ELISA-Hb Pasteur) and one immunodot (GenBio) using quantitative PCR (qPCR) as gold standard, instead of the traditional microscopic agglutination test, for the diagnosis of acute leptospirosis in an endemic area.Between January 2011 and December 2012, a total of 122 patients were diagnosed with leptospirosis, as confirmed by qPCR at the University Hospital of Martinique. Among them, 103 had at least one serum sample available for analysis. Performance of each serological assay was evaluated according to days' post onset of symptoms (DPO) and local species diversity (which included L. santarosai, L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchii, and L. kmetyi). Several thresholds were tested to optimize accuracy. When considering the manufacturer's threshold, the sensitivity of ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot was 75%, 67% and 64%, while specificity was 92%, 98% and 100%, respectively. Moreover, the threshold optimization allowed a significant improvement in specificity for the ELISA Serion from 92% to 99% (p<0.05). During the first 5 DPO, sensitivities were 35%, 30% and 42% for ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot, respectively. However, between 6─10 DPO, these sensitivities dramatically increased to reach 86%, 76% and 67%, respectively. Performances of the three assays were not affected by the species studied.All these serological assays showed the potential for diagnosing leptospirosis after (but not before) 6 days' post onset of symptoms. In a high prevalence setting, where highest specificities are needed, threshold optimizing should be performed for this purpose. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pasteur ENVELOPE(140.099,140.099,-66.625,-66.625) PLOS Neglected Tropical Diseases 11 6 e0005678 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Christophe Courdurie Yohann Le Govic Pascale Bourhy Dorothee Alexer Karine Pailla Rafaelle Theodose Raymond Cesaire Jacques Rosine Patrick Hochedez Claude Olive Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is a potentially life-threatening but curable zoonosis whose prognosis depends on accurate and timely diagnosis. Because of its non-specific clinical presentation, laboratory testing is essential to confirm the diagnosis. Here, we aimed to assess the performance of two enzyme-linked immunosorbent assays (ELISAs) (ELISA Serion and ELISA-Hb Pasteur) and one immunodot (GenBio) using quantitative PCR (qPCR) as gold standard, instead of the traditional microscopic agglutination test, for the diagnosis of acute leptospirosis in an endemic area.Between January 2011 and December 2012, a total of 122 patients were diagnosed with leptospirosis, as confirmed by qPCR at the University Hospital of Martinique. Among them, 103 had at least one serum sample available for analysis. Performance of each serological assay was evaluated according to days' post onset of symptoms (DPO) and local species diversity (which included L. santarosai, L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchii, and L. kmetyi). Several thresholds were tested to optimize accuracy. When considering the manufacturer's threshold, the sensitivity of ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot was 75%, 67% and 64%, while specificity was 92%, 98% and 100%, respectively. Moreover, the threshold optimization allowed a significant improvement in specificity for the ELISA Serion from 92% to 99% (p<0.05). During the first 5 DPO, sensitivities were 35%, 30% and 42% for ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot, respectively. However, between 6─10 DPO, these sensitivities dramatically increased to reach 86%, 76% and 67%, respectively. Performances of the three assays were not affected by the species studied.All these serological assays showed the potential for diagnosing leptospirosis after (but not before) 6 days' post onset of symptoms. In a high prevalence setting, where highest specificities are needed, threshold optimizing should be performed for this purpose. |
format |
Article in Journal/Newspaper |
author |
Christophe Courdurie Yohann Le Govic Pascale Bourhy Dorothee Alexer Karine Pailla Rafaelle Theodose Raymond Cesaire Jacques Rosine Patrick Hochedez Claude Olive |
author_facet |
Christophe Courdurie Yohann Le Govic Pascale Bourhy Dorothee Alexer Karine Pailla Rafaelle Theodose Raymond Cesaire Jacques Rosine Patrick Hochedez Claude Olive |
author_sort |
Christophe Courdurie |
title |
Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
title_short |
Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
title_full |
Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
title_fullStr |
Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
title_full_unstemmed |
Evaluation of different serological assays for early diagnosis of leptospirosis in Martinique (French West Indies). |
title_sort |
evaluation of different serological assays for early diagnosis of leptospirosis in martinique (french west indies). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005678 https://doaj.org/article/16c47021dffc4952aece15aae9e37e52 |
long_lat |
ENVELOPE(140.099,140.099,-66.625,-66.625) |
geographic |
Arctic Pasteur |
geographic_facet |
Arctic Pasteur |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005678 (2017) |
op_relation |
http://europepmc.org/articles/PMC5500375?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005678 https://doaj.org/article/16c47021dffc4952aece15aae9e37e52 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005678 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
6 |
container_start_page |
e0005678 |
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1766345036669124608 |