New synchronization method for Plasmodium falciparum
Abstract Background Plasmodium falciparum is usually asynchronous during in vitro culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cul...
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ftdoajarticles:oai:doaj.org/article:168d5b0223cf4f7b93303c20d655f662 2023-05-15T15:07:45+02:00 New synchronization method for Plasmodium falciparum Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C 2010-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-170 https://doaj.org/article/168d5b0223cf4f7b93303c20d655f662 EN eng BMC http://www.malariajournal.com/content/9/1/170 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-170 1475-2875 https://doaj.org/article/168d5b0223cf4f7b93303c20d655f662 Malaria Journal, Vol 9, Iss 1, p 170 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-170 2022-12-30T22:15:47Z Abstract Background Plasmodium falciparum is usually asynchronous during in vitro culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes. Methods Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites. Results Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents. Conclusions The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C New synchronization method for Plasmodium falciparum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum is usually asynchronous during in vitro culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes. Methods Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites. Results Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents. Conclusions The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle. |
format |
Article in Journal/Newspaper |
author |
Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C |
author_facet |
Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C |
author_sort |
Mwangi Jonathan M |
title |
New synchronization method for Plasmodium falciparum |
title_short |
New synchronization method for Plasmodium falciparum |
title_full |
New synchronization method for Plasmodium falciparum |
title_fullStr |
New synchronization method for Plasmodium falciparum |
title_full_unstemmed |
New synchronization method for Plasmodium falciparum |
title_sort |
new synchronization method for plasmodium falciparum |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-170 https://doaj.org/article/168d5b0223cf4f7b93303c20d655f662 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 170 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/170 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-170 1475-2875 https://doaj.org/article/168d5b0223cf4f7b93303c20d655f662 |
op_doi |
https://doi.org/10.1186/1475-2875-9-170 |
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Malaria Journal |
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9 |
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1 |
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1766339178535059456 |