Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema
Abstract Equine coital exanthema (ECE) caused by equid alphaherpesvirus 3 (EHV-3) is a contagious venereal disease. It is characterized by the formation of papules, vesicles, pustules and ulcers on the external genitals of both mares and stallions. The Icelandic horse is the only breed in Iceland an...
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ftdoajarticles:oai:doaj.org/article:16821da72f574b99b5c85421f150af72 2023-05-15T16:45:44+02:00 Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema Lilja Thorsteinsdóttir Gunnar Örn Guðmundsson Höskuldur Jensson Sigurbjörg Torsteinsdóttir Vilhjálmur Svansson 2021-02-01T00:00:00Z https://doi.org/10.1186/s13028-021-00572-4 https://doaj.org/article/16821da72f574b99b5c85421f150af72 EN eng BMC https://doi.org/10.1186/s13028-021-00572-4 https://doaj.org/toc/1751-0147 doi:10.1186/s13028-021-00572-4 1751-0147 https://doaj.org/article/16821da72f574b99b5c85421f150af72 Acta Veterinaria Scandinavica, Vol 63, Iss 1, Pp 1-4 (2021) DNA polymerase gene Glycoprotein G PCR Sequencing Titration Virus isolation Veterinary medicine SF600-1100 article 2021 ftdoajarticles https://doi.org/10.1186/s13028-021-00572-4 2022-12-31T10:24:33Z Abstract Equine coital exanthema (ECE) caused by equid alphaherpesvirus 3 (EHV-3) is a contagious venereal disease. It is characterized by the formation of papules, vesicles, pustules and ulcers on the external genitals of both mares and stallions. The Icelandic horse is the only breed in Iceland and has lived isolated in the country for over 1000 years. Three types of equine herpesviruses (EHV) have been found in Iceland, EHV-4, EHV-2 and EHV-5, while EHV-1 has never been detected. Symptoms resembling ECE have previous been observed in horses in Iceland, arousing suspicion of EHV-3 infection, but this has never been confirmed using virological methods. Samples were collected from a mare with papules on the vulva and inoculated in primary equine kidney cells. Cytopathic effects developed as rounded cells and syncytial formation. Polymerase chain reaction and sequencing of the partial glycoprotein G and DNA polymerase genes identified the isolated virus as EHV-3. On the basis of the findings, EHV-3 infection was verified for the first time in the native Icelandic horse population. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Acta Veterinaria Scandinavica 63 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
DNA polymerase gene Glycoprotein G PCR Sequencing Titration Virus isolation Veterinary medicine SF600-1100 |
spellingShingle |
DNA polymerase gene Glycoprotein G PCR Sequencing Titration Virus isolation Veterinary medicine SF600-1100 Lilja Thorsteinsdóttir Gunnar Örn Guðmundsson Höskuldur Jensson Sigurbjörg Torsteinsdóttir Vilhjálmur Svansson Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
topic_facet |
DNA polymerase gene Glycoprotein G PCR Sequencing Titration Virus isolation Veterinary medicine SF600-1100 |
description |
Abstract Equine coital exanthema (ECE) caused by equid alphaherpesvirus 3 (EHV-3) is a contagious venereal disease. It is characterized by the formation of papules, vesicles, pustules and ulcers on the external genitals of both mares and stallions. The Icelandic horse is the only breed in Iceland and has lived isolated in the country for over 1000 years. Three types of equine herpesviruses (EHV) have been found in Iceland, EHV-4, EHV-2 and EHV-5, while EHV-1 has never been detected. Symptoms resembling ECE have previous been observed in horses in Iceland, arousing suspicion of EHV-3 infection, but this has never been confirmed using virological methods. Samples were collected from a mare with papules on the vulva and inoculated in primary equine kidney cells. Cytopathic effects developed as rounded cells and syncytial formation. Polymerase chain reaction and sequencing of the partial glycoprotein G and DNA polymerase genes identified the isolated virus as EHV-3. On the basis of the findings, EHV-3 infection was verified for the first time in the native Icelandic horse population. |
format |
Article in Journal/Newspaper |
author |
Lilja Thorsteinsdóttir Gunnar Örn Guðmundsson Höskuldur Jensson Sigurbjörg Torsteinsdóttir Vilhjálmur Svansson |
author_facet |
Lilja Thorsteinsdóttir Gunnar Örn Guðmundsson Höskuldur Jensson Sigurbjörg Torsteinsdóttir Vilhjálmur Svansson |
author_sort |
Lilja Thorsteinsdóttir |
title |
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
title_short |
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
title_full |
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
title_fullStr |
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
title_full_unstemmed |
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema |
title_sort |
isolation of equid alphaherpesvirus 3 from a horse in iceland with equine coital exanthema |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s13028-021-00572-4 https://doaj.org/article/16821da72f574b99b5c85421f150af72 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Acta Veterinaria Scandinavica, Vol 63, Iss 1, Pp 1-4 (2021) |
op_relation |
https://doi.org/10.1186/s13028-021-00572-4 https://doaj.org/toc/1751-0147 doi:10.1186/s13028-021-00572-4 1751-0147 https://doaj.org/article/16821da72f574b99b5c85421f150af72 |
op_doi |
https://doi.org/10.1186/s13028-021-00572-4 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
63 |
container_issue |
1 |
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1766035892318765056 |