Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad
BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscu...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:1679c6bdf4124f028e1275042b8eff70 2023-05-15T15:07:23+02:00 Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad Sandro Rostelato-Ferreira Cháriston A Dal Belo Gildo B Leite Stephen Hyslop Léa Rodrigues-Simioni 2014-09-01T00:00:00Z https://doi.org/10.1186/1678-9199-20-30 https://doaj.org/article/1679c6bdf4124f028e1275042b8eff70 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200332&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-30 https://doaj.org/article/1679c6bdf4124f028e1275042b8eff70 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) Neurotransmitter release Ouabain Presynaptic Rhinella schneideri Toad venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1186/1678-9199-20-30 2022-12-31T14:56:19Z BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity.MethodsThe neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements.ResultsVenom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological evaluations showed that the extract (50 μg/mL) significantly augmented the frequency of miniature end-plate potential (from 38 ± 3.5 to 88 ± 15 after 60 minutes; n = 5; p< 0.05) and quantal content (from 128 ± 13 to 272 ± 34 after five minutes; n = 5; p< 0.05). Pretreatment with ouabain (1 μg/mL) for five minutes prevented the increase in quantal content (117 ± 18 and 154 ± 33 after five and 60 minutes, respectively).ConclusionThese results indicate that the methanolic extract of R. schneiderivenom acts primarily presynaptically to enhance neurotransmitter release in mouse phrenic-diaphragm preparations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 20 1 30 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
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Neurotransmitter release Ouabain Presynaptic Rhinella schneideri Toad venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Neurotransmitter release Ouabain Presynaptic Rhinella schneideri Toad venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Sandro Rostelato-Ferreira Cháriston A Dal Belo Gildo B Leite Stephen Hyslop Léa Rodrigues-Simioni Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
topic_facet |
Neurotransmitter release Ouabain Presynaptic Rhinella schneideri Toad venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity.MethodsThe neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements.ResultsVenom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological evaluations showed that the extract (50 μg/mL) significantly augmented the frequency of miniature end-plate potential (from 38 ± 3.5 to 88 ± 15 after 60 minutes; n = 5; p< 0.05) and quantal content (from 128 ± 13 to 272 ± 34 after five minutes; n = 5; p< 0.05). Pretreatment with ouabain (1 μg/mL) for five minutes prevented the increase in quantal content (117 ± 18 and 154 ± 33 after five and 60 minutes, respectively).ConclusionThese results indicate that the methanolic extract of R. schneiderivenom acts primarily presynaptically to enhance neurotransmitter release in mouse phrenic-diaphragm preparations. |
format |
Article in Journal/Newspaper |
author |
Sandro Rostelato-Ferreira Cháriston A Dal Belo Gildo B Leite Stephen Hyslop Léa Rodrigues-Simioni |
author_facet |
Sandro Rostelato-Ferreira Cháriston A Dal Belo Gildo B Leite Stephen Hyslop Léa Rodrigues-Simioni |
author_sort |
Sandro Rostelato-Ferreira |
title |
Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
title_short |
Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
title_full |
Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
title_fullStr |
Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
title_full_unstemmed |
Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad |
title_sort |
presynaptic neuromuscular action of a methanolic extract from the venom of rhinella schneideri toad |
publisher |
SciELO |
publishDate |
2014 |
url |
https://doi.org/10.1186/1678-9199-20-30 https://doaj.org/article/1679c6bdf4124f028e1275042b8eff70 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200332&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-30 https://doaj.org/article/1679c6bdf4124f028e1275042b8eff70 |
op_doi |
https://doi.org/10.1186/1678-9199-20-30 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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20 |
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1 |
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30 |
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1766338908695560192 |