Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.
BACKGROUND:Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this stu...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0007116 https://doaj.org/article/163e41f8c7064868ad9017dbd9ca4e9b |
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ftdoajarticles:oai:doaj.org/article:163e41f8c7064868ad9017dbd9ca4e9b 2023-05-15T15:14:34+02:00 Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. Kanya C Long Juan Sulca Isabel Bazan Helvio Astete Hugo L Jaba Crystyan Siles Claudine Kocher Stalin Vilcarromero Julia Schwarz Karin S Escobedo-Vargas Fanny Castro-Llanos Leslye Angulo Guadalupe Flores Cesar Ramal-Asayag Eric S Halsey Robert D Hontz Valerie A Paz-Soldan Thomas W Scott Louis Lambrechts Amy C Morrison 2019-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007116 https://doaj.org/article/163e41f8c7064868ad9017dbd9ca4e9b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6388938?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007116 https://doaj.org/article/163e41f8c7064868ad9017dbd9ca4e9b PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007116 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007116 2022-12-31T03:01:41Z BACKGROUND:Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru. METHODOLOGY/PRINCIPAL FINDINGS:Our study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. Mosquitoes were tested after approximately 14 days of extrinsic incubation for DENV infection and dissemination. A total of 58 participants, with viremias ranging from 1.3 × 10(2) to 2.9 × 10(6) focus-forming units per mL of serum, participated in one or more feeding methods. DENV infection and dissemination rates were not significantly different following direct and indirect-EDTA feeding; however, they were significantly lower for mosquitoes that fed indirectly on blood with no additive. Relative to direct feeding, infection rates showed greater variation following indirect-EDTA than indirect-no additive feeding. Dissemination rates were similar across all feeding methods. No differences were detected in DENV infection or dissemination rates in mosquitoes fed directly on participants with different dengue illness severity. CONCLUSIONS/SIGNIFICANCE:Our study demonstrates the feasibility of using direct and indirect feeding methods for field-based studies on vector competence. Direct mosquito feeding is preferable in terms of logistical ease, biosecurity, and reliability. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 2 e0007116 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kanya C Long Juan Sulca Isabel Bazan Helvio Astete Hugo L Jaba Crystyan Siles Claudine Kocher Stalin Vilcarromero Julia Schwarz Karin S Escobedo-Vargas Fanny Castro-Llanos Leslye Angulo Guadalupe Flores Cesar Ramal-Asayag Eric S Halsey Robert D Hontz Valerie A Paz-Soldan Thomas W Scott Louis Lambrechts Amy C Morrison Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru. METHODOLOGY/PRINCIPAL FINDINGS:Our study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. Mosquitoes were tested after approximately 14 days of extrinsic incubation for DENV infection and dissemination. A total of 58 participants, with viremias ranging from 1.3 × 10(2) to 2.9 × 10(6) focus-forming units per mL of serum, participated in one or more feeding methods. DENV infection and dissemination rates were not significantly different following direct and indirect-EDTA feeding; however, they were significantly lower for mosquitoes that fed indirectly on blood with no additive. Relative to direct feeding, infection rates showed greater variation following indirect-EDTA than indirect-no additive feeding. Dissemination rates were similar across all feeding methods. No differences were detected in DENV infection or dissemination rates in mosquitoes fed directly on participants with different dengue illness severity. CONCLUSIONS/SIGNIFICANCE:Our study demonstrates the feasibility of using direct and indirect feeding methods for field-based studies on vector competence. Direct mosquito feeding is preferable in terms of logistical ease, biosecurity, and reliability. |
format |
Article in Journal/Newspaper |
author |
Kanya C Long Juan Sulca Isabel Bazan Helvio Astete Hugo L Jaba Crystyan Siles Claudine Kocher Stalin Vilcarromero Julia Schwarz Karin S Escobedo-Vargas Fanny Castro-Llanos Leslye Angulo Guadalupe Flores Cesar Ramal-Asayag Eric S Halsey Robert D Hontz Valerie A Paz-Soldan Thomas W Scott Louis Lambrechts Amy C Morrison |
author_facet |
Kanya C Long Juan Sulca Isabel Bazan Helvio Astete Hugo L Jaba Crystyan Siles Claudine Kocher Stalin Vilcarromero Julia Schwarz Karin S Escobedo-Vargas Fanny Castro-Llanos Leslye Angulo Guadalupe Flores Cesar Ramal-Asayag Eric S Halsey Robert D Hontz Valerie A Paz-Soldan Thomas W Scott Louis Lambrechts Amy C Morrison |
author_sort |
Kanya C Long |
title |
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
title_short |
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
title_full |
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
title_fullStr |
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
title_full_unstemmed |
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru. |
title_sort |
feasibility of feeding aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in iquitos, peru. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007116 https://doaj.org/article/163e41f8c7064868ad9017dbd9ca4e9b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007116 (2019) |
op_relation |
http://europepmc.org/articles/PMC6388938?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007116 https://doaj.org/article/163e41f8c7064868ad9017dbd9ca4e9b |
op_doi |
https://doi.org/10.1371/journal.pntd.0007116 |
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PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
2 |
container_start_page |
e0007116 |
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1766345013243936768 |