Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria.
Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterat...
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ftdoajarticles:oai:doaj.org/article:160f12596c5945c9b9e94573f2120244 2023-05-15T15:10:28+02:00 Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. João Conrado Khouri Dos-Santos João Luiz Silva-Filho Carla C Judice Ana Carolina Andrade Vitor Kayano Júlio Aliberti Ricardo Khouri Diógenes S de Lima Helder Nakaya Marcus Vinicius Guimarães Lacerda Erich Vinicius De Paula Stefanie Costa Pinto Lopes Fabio Trindade Maranhão Costa 2020-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007656 https://doaj.org/article/160f12596c5945c9b9e94573f2120244 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007656 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007656 https://doaj.org/article/160f12596c5945c9b9e94573f2120244 PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0007656 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007656 2022-12-31T13:50:13Z Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial activation markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson's correlation coefficients to generate correlation networks. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406-651] vs. 1029 [732-1267] pg/mL, P = 0.0001) were significantly higher in patients, while P-selectin and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets of P. vivax patients when compared to controls. Although absolute platelet activation was not strong in uncomplicated vivax malaria, markers of platelet activity and production were correlated with higher endothelial cell activation, especially in a specific subset of patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 7 e0007656 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 João Conrado Khouri Dos-Santos João Luiz Silva-Filho Carla C Judice Ana Carolina Andrade Vitor Kayano Júlio Aliberti Ricardo Khouri Diógenes S de Lima Helder Nakaya Marcus Vinicius Guimarães Lacerda Erich Vinicius De Paula Stefanie Costa Pinto Lopes Fabio Trindade Maranhão Costa Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial activation markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson's correlation coefficients to generate correlation networks. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406-651] vs. 1029 [732-1267] pg/mL, P = 0.0001) were significantly higher in patients, while P-selectin and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets of P. vivax patients when compared to controls. Although absolute platelet activation was not strong in uncomplicated vivax malaria, markers of platelet activity and production were correlated with higher endothelial cell activation, especially in a specific subset of patients. |
format |
Article in Journal/Newspaper |
author |
João Conrado Khouri Dos-Santos João Luiz Silva-Filho Carla C Judice Ana Carolina Andrade Vitor Kayano Júlio Aliberti Ricardo Khouri Diógenes S de Lima Helder Nakaya Marcus Vinicius Guimarães Lacerda Erich Vinicius De Paula Stefanie Costa Pinto Lopes Fabio Trindade Maranhão Costa |
author_facet |
João Conrado Khouri Dos-Santos João Luiz Silva-Filho Carla C Judice Ana Carolina Andrade Vitor Kayano Júlio Aliberti Ricardo Khouri Diógenes S de Lima Helder Nakaya Marcus Vinicius Guimarães Lacerda Erich Vinicius De Paula Stefanie Costa Pinto Lopes Fabio Trindade Maranhão Costa |
author_sort |
João Conrado Khouri Dos-Santos |
title |
Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
title_short |
Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
title_full |
Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
title_fullStr |
Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
title_full_unstemmed |
Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. |
title_sort |
platelet disturbances correlate with endothelial cell activation in uncomplicated plasmodium vivax malaria. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0007656 https://doaj.org/article/160f12596c5945c9b9e94573f2120244 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0007656 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007656 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007656 https://doaj.org/article/160f12596c5945c9b9e94573f2120244 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007656 |
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PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0007656 |
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