Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker
Objective: To understand the effects of combination treatment of pamidronate with isolated Quercus infectoria semi-purified fraction (QIsm-F) on human foetal osteoblast cell model (hFOB 1.19 cell line) through assessment of Runt related transcription fraction-2 (Runx2) and Osterix (Osx). Methods: Th...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:160c172e6b20472e866dadf68b78fcb1 2023-05-15T15:09:44+02:00 Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker Abdullah Amira Raudhah Hapidin Hermizi Abdullah Hasmah 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.233007 https://doaj.org/article/160c172e6b20472e866dadf68b78fcb1 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=5;spage=261;epage=267;aulast=Raudhah https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.233007 https://doaj.org/article/160c172e6b20472e866dadf68b78fcb1 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 5, Pp 261-267 (2018) pamidronate runx2 osterix osteoblast quercus infectoria semi-purified fraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.233007 2022-12-30T21:04:51Z Objective: To understand the effects of combination treatment of pamidronate with isolated Quercus infectoria semi-purified fraction (QIsm-F) on human foetal osteoblast cell model (hFOB 1.19 cell line) through assessment of Runt related transcription fraction-2 (Runx2) and Osterix (Osx). Methods: The isolation and purification of QIsm-F were conducted by chromatographic technique. In order to assess relative efficacy of QIsm-F to the osteoblast model, the determination of half maximal effective concentration (EC50) was performed by MTT assay. hFOB 1.19 cells were cultured in DMEM F-12 and supplemented with 10% fetal bovine serum along with 1% penicillin-streptomycin incubated in 5% CO2 at 37 °C. Expression of Runx2 and Osx was assessed through western blotting and confirmed with immunofluorescence staining. Results: Results of western blot analysis and immunofluorescence staining demonstrated that compared to hFOB 1.19 cells treated with single individual treatment of QIsm-F and control groups, levels of Runx2 and Osx were elevated with higher fluorescence intensity and more rapid proliferation in hFOB 1.19 cells treated with combined treatment of QIsm-F and pamidronate. Conclusions: The finding demonstrates the synergistic effect between osteoporotic drug pamidronate and established QIsm-F. The combination treatment helps increase the efficiency of pamidronate acting on osteoblast cells by stimulating osteoblast proliferation and differentiation via expression of Runx2 and Osx. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 5 261 |
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Directory of Open Access Journals: DOAJ Articles |
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pamidronate runx2 osterix osteoblast quercus infectoria semi-purified fraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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pamidronate runx2 osterix osteoblast quercus infectoria semi-purified fraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Abdullah Amira Raudhah Hapidin Hermizi Abdullah Hasmah Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
topic_facet |
pamidronate runx2 osterix osteoblast quercus infectoria semi-purified fraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To understand the effects of combination treatment of pamidronate with isolated Quercus infectoria semi-purified fraction (QIsm-F) on human foetal osteoblast cell model (hFOB 1.19 cell line) through assessment of Runt related transcription fraction-2 (Runx2) and Osterix (Osx). Methods: The isolation and purification of QIsm-F were conducted by chromatographic technique. In order to assess relative efficacy of QIsm-F to the osteoblast model, the determination of half maximal effective concentration (EC50) was performed by MTT assay. hFOB 1.19 cells were cultured in DMEM F-12 and supplemented with 10% fetal bovine serum along with 1% penicillin-streptomycin incubated in 5% CO2 at 37 °C. Expression of Runx2 and Osx was assessed through western blotting and confirmed with immunofluorescence staining. Results: Results of western blot analysis and immunofluorescence staining demonstrated that compared to hFOB 1.19 cells treated with single individual treatment of QIsm-F and control groups, levels of Runx2 and Osx were elevated with higher fluorescence intensity and more rapid proliferation in hFOB 1.19 cells treated with combined treatment of QIsm-F and pamidronate. Conclusions: The finding demonstrates the synergistic effect between osteoporotic drug pamidronate and established QIsm-F. The combination treatment helps increase the efficiency of pamidronate acting on osteoblast cells by stimulating osteoblast proliferation and differentiation via expression of Runx2 and Osx. |
format |
Article in Journal/Newspaper |
author |
Abdullah Amira Raudhah Hapidin Hermizi Abdullah Hasmah |
author_facet |
Abdullah Amira Raudhah Hapidin Hermizi Abdullah Hasmah |
author_sort |
Abdullah Amira Raudhah |
title |
Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
title_short |
Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
title_full |
Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
title_fullStr |
Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
title_full_unstemmed |
Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker |
title_sort |
combination treatment of bisphosphonate (pamidronate) and quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of osterix and runx2 marker |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.233007 https://doaj.org/article/160c172e6b20472e866dadf68b78fcb1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 5, Pp 261-267 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=5;spage=261;epage=267;aulast=Raudhah https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.233007 https://doaj.org/article/160c172e6b20472e866dadf68b78fcb1 |
op_doi |
https://doi.org/10.4103/2221-1691.233007 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
5 |
container_start_page |
261 |
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1766340865368784896 |