Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action
Abstract Background Malaria remains one of the most virulent and deadliest parasitic disease in the world, particularly in Africa and Southeast Asia. Widespread occurrence of artemisinin-resistant Plasmodium falciparum strains from the Greater Mekong Subregion is alarming. This hinders the national...
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ftdoajarticles:oai:doaj.org/article:15f984ed79ea451c8ecea3740d4ba80a 2023-05-15T15:15:22+02:00 Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action Jing Wei Lai Mohd Jamil Maah Kong Wai Tan Rozie Sarip Yvonne Ai Lian Lim Rakesh Ganguly Loke Tim Khaw Chew Hee Ng 2022-12-01T00:00:00Z https://doi.org/10.1186/s12936-022-04406-0 https://doaj.org/article/15f984ed79ea451c8ecea3740d4ba80a EN eng BMC https://doi.org/10.1186/s12936-022-04406-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04406-0 1475-2875 https://doaj.org/article/15f984ed79ea451c8ecea3740d4ba80a Malaria Journal, Vol 21, Iss 1, Pp 1-21 (2022) Copper complex Zinc complex Antimalarial Plasmodium falciparum Reactive oxygen species Depolarization of mitochondrial membrane potential Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04406-0 2022-12-30T20:56:32Z Abstract Background Malaria remains one of the most virulent and deadliest parasitic disease in the world, particularly in Africa and Southeast Asia. Widespread occurrence of artemisinin-resistant Plasmodium falciparum strains from the Greater Mekong Subregion is alarming. This hinders the national economies, as well as being a major drawback in the effective control and elimination of malaria worldwide. Clearly, an effective anti-malarial drug is urgently needed. Methods The dinuclear and mononuclear copper(II) and zinc(II) complexes were synthesized in ethanolic solution and characterized by various physical measurements (FTIR, CHN elemental analysis, solubility, ESI-MS, UV-Visible, conductivity and magnetic moment, and NMR). X-ray crystal structure of the dicopper(II) complex was determined. The in vitro haemolytic activities of these metal complexes were evaluated spectroscopically on B+ blood while the anti-malarial potency was performed in vitro on blood stage drug-sensitive Plasmodium falciparum 3D7 (Pf3D7) and artemisinin-resistant Plasmodium falciparum IPC5202 (Pf5202) with fluorescence dye. Mode of action of metal complexes were conducted to determine the formation of reactive oxygen species using PNDA and DCFH-DA dyes, JC-1 depolarization of mitochondrial membrane potential, malarial 20S proteasome inhibition with parasite lysate, and morphological studies using Giemsa and Hoechst stains. Results Copper(II) complexes showed anti-malarial potency against both Pf3D7 and Pf5202 in sub-micromolar to micromolar range. The zinc(II) complexes were effective against Pf3D7 with excellent therapeutic index but encountered total resistance against Pf5202. Among the four, the dinuclear copper(II) complex was the most potent against both strains. The zinc(II) complexes caused no haemolysis of RBC while copper(II) complexes induced increased haemolysis with increasing concentration. Further mechanistic studies of both copper(II) complexes on both Pf3D7 and Pf5202 strains showed induction of ROS, 20S malarial ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Copper complex Zinc complex Antimalarial Plasmodium falciparum Reactive oxygen species Depolarization of mitochondrial membrane potential Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Copper complex Zinc complex Antimalarial Plasmodium falciparum Reactive oxygen species Depolarization of mitochondrial membrane potential Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jing Wei Lai Mohd Jamil Maah Kong Wai Tan Rozie Sarip Yvonne Ai Lian Lim Rakesh Ganguly Loke Tim Khaw Chew Hee Ng Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
topic_facet |
Copper complex Zinc complex Antimalarial Plasmodium falciparum Reactive oxygen species Depolarization of mitochondrial membrane potential Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria remains one of the most virulent and deadliest parasitic disease in the world, particularly in Africa and Southeast Asia. Widespread occurrence of artemisinin-resistant Plasmodium falciparum strains from the Greater Mekong Subregion is alarming. This hinders the national economies, as well as being a major drawback in the effective control and elimination of malaria worldwide. Clearly, an effective anti-malarial drug is urgently needed. Methods The dinuclear and mononuclear copper(II) and zinc(II) complexes were synthesized in ethanolic solution and characterized by various physical measurements (FTIR, CHN elemental analysis, solubility, ESI-MS, UV-Visible, conductivity and magnetic moment, and NMR). X-ray crystal structure of the dicopper(II) complex was determined. The in vitro haemolytic activities of these metal complexes were evaluated spectroscopically on B+ blood while the anti-malarial potency was performed in vitro on blood stage drug-sensitive Plasmodium falciparum 3D7 (Pf3D7) and artemisinin-resistant Plasmodium falciparum IPC5202 (Pf5202) with fluorescence dye. Mode of action of metal complexes were conducted to determine the formation of reactive oxygen species using PNDA and DCFH-DA dyes, JC-1 depolarization of mitochondrial membrane potential, malarial 20S proteasome inhibition with parasite lysate, and morphological studies using Giemsa and Hoechst stains. Results Copper(II) complexes showed anti-malarial potency against both Pf3D7 and Pf5202 in sub-micromolar to micromolar range. The zinc(II) complexes were effective against Pf3D7 with excellent therapeutic index but encountered total resistance against Pf5202. Among the four, the dinuclear copper(II) complex was the most potent against both strains. The zinc(II) complexes caused no haemolysis of RBC while copper(II) complexes induced increased haemolysis with increasing concentration. Further mechanistic studies of both copper(II) complexes on both Pf3D7 and Pf5202 strains showed induction of ROS, 20S malarial ... |
format |
Article in Journal/Newspaper |
author |
Jing Wei Lai Mohd Jamil Maah Kong Wai Tan Rozie Sarip Yvonne Ai Lian Lim Rakesh Ganguly Loke Tim Khaw Chew Hee Ng |
author_facet |
Jing Wei Lai Mohd Jamil Maah Kong Wai Tan Rozie Sarip Yvonne Ai Lian Lim Rakesh Ganguly Loke Tim Khaw Chew Hee Ng |
author_sort |
Jing Wei Lai |
title |
Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
title_short |
Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
title_full |
Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
title_fullStr |
Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
title_full_unstemmed |
Dinuclear and mononuclear metal(II) polypyridyl complexes against drug-sensitive and drug-resistant Plasmodium falciparum and their mode of action |
title_sort |
dinuclear and mononuclear metal(ii) polypyridyl complexes against drug-sensitive and drug-resistant plasmodium falciparum and their mode of action |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04406-0 https://doaj.org/article/15f984ed79ea451c8ecea3740d4ba80a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-21 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04406-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04406-0 1475-2875 https://doaj.org/article/15f984ed79ea451c8ecea3740d4ba80a |
op_doi |
https://doi.org/10.1186/s12936-022-04406-0 |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
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1766345734748110848 |