Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium
Abstract Background Bartonella spp. are vector-borne pathogens transmitted to humans via blood-sucking arthropods. Rodents such as the black rat (Rattus rattus) and Norway rat (R. norvegicus) are thought to be the main reservoirs. An infection with rodent-associated Bartonella spp. may cause severe...
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ftdoajarticles:oai:doaj.org/article:15ecaea1bdd94dddaec2f82505bf507e 2023-05-15T18:05:41+02:00 Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium Maria Krügel Martin Pfeffer Nina Król Christian Imholt Kristof Baert Rainer G. Ulrich Anna Obiegala 2020-05-01T00:00:00Z https://doi.org/10.1186/s13071-020-04098-y https://doaj.org/article/15ecaea1bdd94dddaec2f82505bf507e EN eng BMC http://link.springer.com/article/10.1186/s13071-020-04098-y https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04098-y 1756-3305 https://doaj.org/article/15ecaea1bdd94dddaec2f82505bf507e Parasites & Vectors, Vol 13, Iss 1, Pp 1-12 (2020) Bartonella Bartonella tribocorum Bartonella doshiae Bartonella grahamii Belgium Rodents Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s13071-020-04098-y 2022-12-31T10:12:18Z Abstract Background Bartonella spp. are vector-borne pathogens transmitted to humans via blood-sucking arthropods. Rodents such as the black rat (Rattus rattus) and Norway rat (R. norvegicus) are thought to be the main reservoirs. An infection with rodent-associated Bartonella spp. may cause severe symptoms in humans such as endocarditis and neuroretinitis. The current knowledge of Bartonella prevalence in rats from western Europe is scarce. Methods Rats and a few other rodent by-catches were trapped in the context of a rodenticide resistance study at different sites in Flanders, Belgium. During dissection, biometric data were collected, and spleen tissues were taken. DNA was extracted from spleen samples and tested for Bartonella spp. by conventional generic polymerase chain reaction (PCR). To determine the Bartonella species, a selected number of amplicons were sequenced and compared with GenBank entries. Results In total, 1123 rodents were trapped. The predominate species was R. norvegicus (99.64%). Other rodents trapped included: two water voles (Arvicola amphibius, 0.18%); one colour rat (R. norvegicus forma domestica, 0.09%); and one muskrat (Ondatra zibethicus, 0.09%). PCR analysis of 1097 rodents resulted in 410 (37.37%, 95% CI: 34.50–40.31%) Bartonella spp. DNA-positive samples. Bartonella tribocorum (94.68%, 95% CI: 88.02–98.25%) was the most frequently detected Bartonella species, followed by B. grahamii (3.19%, 95% CI: 0.66–9.04%) and B. doshiae (1.06%, 95% CI: 0.03–5.79%). An uncultured Bartonella species occurred in one water vole (1.06%, 95% CI: 0.03–5.79%). There was a significantly higher Bartonella prevalence in older rats compared to juveniles and a significant difference in Bartonella prevalence concerning the localisation of trapping sites. In contrast, there was no statistically significant difference in Bartonella prevalence regarding sex, degree of urbanisation and season. Conclusions Based on the high prevalence found, we conclude that the Norway rat seems to be a key reservoir host for ... Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Norway Parasites & Vectors 13 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bartonella Bartonella tribocorum Bartonella doshiae Bartonella grahamii Belgium Rodents Infectious and parasitic diseases RC109-216 |
spellingShingle |
Bartonella Bartonella tribocorum Bartonella doshiae Bartonella grahamii Belgium Rodents Infectious and parasitic diseases RC109-216 Maria Krügel Martin Pfeffer Nina Król Christian Imholt Kristof Baert Rainer G. Ulrich Anna Obiegala Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
topic_facet |
Bartonella Bartonella tribocorum Bartonella doshiae Bartonella grahamii Belgium Rodents Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Bartonella spp. are vector-borne pathogens transmitted to humans via blood-sucking arthropods. Rodents such as the black rat (Rattus rattus) and Norway rat (R. norvegicus) are thought to be the main reservoirs. An infection with rodent-associated Bartonella spp. may cause severe symptoms in humans such as endocarditis and neuroretinitis. The current knowledge of Bartonella prevalence in rats from western Europe is scarce. Methods Rats and a few other rodent by-catches were trapped in the context of a rodenticide resistance study at different sites in Flanders, Belgium. During dissection, biometric data were collected, and spleen tissues were taken. DNA was extracted from spleen samples and tested for Bartonella spp. by conventional generic polymerase chain reaction (PCR). To determine the Bartonella species, a selected number of amplicons were sequenced and compared with GenBank entries. Results In total, 1123 rodents were trapped. The predominate species was R. norvegicus (99.64%). Other rodents trapped included: two water voles (Arvicola amphibius, 0.18%); one colour rat (R. norvegicus forma domestica, 0.09%); and one muskrat (Ondatra zibethicus, 0.09%). PCR analysis of 1097 rodents resulted in 410 (37.37%, 95% CI: 34.50–40.31%) Bartonella spp. DNA-positive samples. Bartonella tribocorum (94.68%, 95% CI: 88.02–98.25%) was the most frequently detected Bartonella species, followed by B. grahamii (3.19%, 95% CI: 0.66–9.04%) and B. doshiae (1.06%, 95% CI: 0.03–5.79%). An uncultured Bartonella species occurred in one water vole (1.06%, 95% CI: 0.03–5.79%). There was a significantly higher Bartonella prevalence in older rats compared to juveniles and a significant difference in Bartonella prevalence concerning the localisation of trapping sites. In contrast, there was no statistically significant difference in Bartonella prevalence regarding sex, degree of urbanisation and season. Conclusions Based on the high prevalence found, we conclude that the Norway rat seems to be a key reservoir host for ... |
format |
Article in Journal/Newspaper |
author |
Maria Krügel Martin Pfeffer Nina Król Christian Imholt Kristof Baert Rainer G. Ulrich Anna Obiegala |
author_facet |
Maria Krügel Martin Pfeffer Nina Król Christian Imholt Kristof Baert Rainer G. Ulrich Anna Obiegala |
author_sort |
Maria Krügel |
title |
Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
title_short |
Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
title_full |
Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
title_fullStr |
Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
title_full_unstemmed |
Rats as potential reservoirs for neglected zoonotic Bartonella species in Flanders, Belgium |
title_sort |
rats as potential reservoirs for neglected zoonotic bartonella species in flanders, belgium |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s13071-020-04098-y https://doaj.org/article/15ecaea1bdd94dddaec2f82505bf507e |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Parasites & Vectors, Vol 13, Iss 1, Pp 1-12 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s13071-020-04098-y https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04098-y 1756-3305 https://doaj.org/article/15ecaea1bdd94dddaec2f82505bf507e |
op_doi |
https://doi.org/10.1186/s13071-020-04098-y |
container_title |
Parasites & Vectors |
container_volume |
13 |
container_issue |
1 |
_version_ |
1766177190202834944 |