Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso
Abstract Background Dual hrp2/hrp3 genes deletions in P. falciparum isolates are increasingly reported in malaria-endemic countries and can produce false negative RDT results leading to inadequate case management. Data on the frequency of hrp2/hrp3 deleted parasites are rarely available and it has b...
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ftdoajarticles:oai:doaj.org/article:15d8cbd1880a40f3a9d23fd0ad528d32 2024-01-07T09:42:02+01:00 Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso Casimire Wendlamita Tarama Harouna Soré Mafama Siribié Siaka Débé Réné Kinda Wendyam Gérard Nonkani Farida Tiendrebeogo Winnie Bantango Kassoum Yira Esther Yéri Hien Moussa Wandaogo Guelbéogo Yves Traoré Didier Ménard Adama Gansané 2023-11-01T00:00:00Z https://doi.org/10.1186/s12936-023-04796-9 https://doaj.org/article/15d8cbd1880a40f3a9d23fd0ad528d32 EN eng BMC https://doi.org/10.1186/s12936-023-04796-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04796-9 1475-2875 https://doaj.org/article/15d8cbd1880a40f3a9d23fd0ad528d32 Malaria Journal, Vol 22, Iss 1, Pp 1-8 (2023) Malaria Plasmodium falciparum hrp2 hrp3 Burkina Faso Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04796-9 2023-12-10T01:48:34Z Abstract Background Dual hrp2/hrp3 genes deletions in P. falciparum isolates are increasingly reported in malaria-endemic countries and can produce false negative RDT results leading to inadequate case management. Data on the frequency of hrp2/hrp3 deleted parasites are rarely available and it has become necessary to investigate the issue in Burkina Faso. Methods Plasmodium falciparum-positive dried blood spots were collected during a cross-sectional household survey of the malaria asymptomatic children from Orodara, Gaoua, and Banfora. Amplicons from the target regions (exon 2 of hrp2 and hrp3 genes) were generated using multiplexed nested PCR and sequenced according to Illumina’s MiSeq protocol. Results A total of 251 microscopically positive parasite isolates were sequenced to detect hrp2 and hrp3 gene deletions. The proportion of RDTs negative cases among microscopy positive slides was 12.7% (32/251). The highest prevalence of negative RDTs was found in Orodara 14.3% (5/35), followed by Gaoua 13.1%(24/183), and Banfora 9.1% (3/33). The study found that 95.6% of the parasite isolates were wild type hrp2/ hrp3 while 4.4% (11/251) had a single hrp2 deletion. Of the 11 hrp2 deletion samples, 2 samples were RDT negative (mean parasitaemia was 83 parasites/ μL) while 9 samples were RDT positive with a mean parasitaemia of 520 parasites /μL (CI95%: 192–1239). The highest frequency of hrp2 deletion 4/35 (11.4%) was found in Orodara, while it was similar in the other two sites (< 3.5%). No single deletion of the hrp3 or dual deletion hrp2/3 gene was detected in this study. Conclusion These results demonstrate that P. falciparum isolates lacking hrp2 genes are present in 4.4% of samples obtained from the asymptomatic children population in three sites in Burkina Faso. These parasites are circulating and causing malaria, but they are also still detectable by HRP2-based RTDs due to the presence of the intact pfhrp3 gene. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Plasmodium falciparum hrp2 hrp3 Burkina Faso Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium falciparum hrp2 hrp3 Burkina Faso Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Casimire Wendlamita Tarama Harouna Soré Mafama Siribié Siaka Débé Réné Kinda Wendyam Gérard Nonkani Farida Tiendrebeogo Winnie Bantango Kassoum Yira Esther Yéri Hien Moussa Wandaogo Guelbéogo Yves Traoré Didier Ménard Adama Gansané Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
topic_facet |
Malaria Plasmodium falciparum hrp2 hrp3 Burkina Faso Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Dual hrp2/hrp3 genes deletions in P. falciparum isolates are increasingly reported in malaria-endemic countries and can produce false negative RDT results leading to inadequate case management. Data on the frequency of hrp2/hrp3 deleted parasites are rarely available and it has become necessary to investigate the issue in Burkina Faso. Methods Plasmodium falciparum-positive dried blood spots were collected during a cross-sectional household survey of the malaria asymptomatic children from Orodara, Gaoua, and Banfora. Amplicons from the target regions (exon 2 of hrp2 and hrp3 genes) were generated using multiplexed nested PCR and sequenced according to Illumina’s MiSeq protocol. Results A total of 251 microscopically positive parasite isolates were sequenced to detect hrp2 and hrp3 gene deletions. The proportion of RDTs negative cases among microscopy positive slides was 12.7% (32/251). The highest prevalence of negative RDTs was found in Orodara 14.3% (5/35), followed by Gaoua 13.1%(24/183), and Banfora 9.1% (3/33). The study found that 95.6% of the parasite isolates were wild type hrp2/ hrp3 while 4.4% (11/251) had a single hrp2 deletion. Of the 11 hrp2 deletion samples, 2 samples were RDT negative (mean parasitaemia was 83 parasites/ μL) while 9 samples were RDT positive with a mean parasitaemia of 520 parasites /μL (CI95%: 192–1239). The highest frequency of hrp2 deletion 4/35 (11.4%) was found in Orodara, while it was similar in the other two sites (< 3.5%). No single deletion of the hrp3 or dual deletion hrp2/3 gene was detected in this study. Conclusion These results demonstrate that P. falciparum isolates lacking hrp2 genes are present in 4.4% of samples obtained from the asymptomatic children population in three sites in Burkina Faso. These parasites are circulating and causing malaria, but they are also still detectable by HRP2-based RTDs due to the presence of the intact pfhrp3 gene. |
format |
Article in Journal/Newspaper |
author |
Casimire Wendlamita Tarama Harouna Soré Mafama Siribié Siaka Débé Réné Kinda Wendyam Gérard Nonkani Farida Tiendrebeogo Winnie Bantango Kassoum Yira Esther Yéri Hien Moussa Wandaogo Guelbéogo Yves Traoré Didier Ménard Adama Gansané |
author_facet |
Casimire Wendlamita Tarama Harouna Soré Mafama Siribié Siaka Débé Réné Kinda Wendyam Gérard Nonkani Farida Tiendrebeogo Winnie Bantango Kassoum Yira Esther Yéri Hien Moussa Wandaogo Guelbéogo Yves Traoré Didier Ménard Adama Gansané |
author_sort |
Casimire Wendlamita Tarama |
title |
Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
title_short |
Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
title_full |
Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
title_fullStr |
Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
title_full_unstemmed |
Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso |
title_sort |
assessing the histidine-rich protein 2/3 gene deletion in plasmodium falciparum isolates from burkina faso |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04796-9 https://doaj.org/article/15d8cbd1880a40f3a9d23fd0ad528d32 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-8 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04796-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04796-9 1475-2875 https://doaj.org/article/15d8cbd1880a40f3a9d23fd0ad528d32 |
op_doi |
https://doi.org/10.1186/s12936-023-04796-9 |
container_title |
Malaria Journal |
container_volume |
22 |
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1 |
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1787422888516648960 |