Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans
In order to explore the antioxidant and anti-aging effects and underlying mechanism of Antarctic krill phospholipids, the antioxidant capacity in vitro of krill phospholipids was evaluated by radical scavenging capacities against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2’-azino-bis(3-ethylbenzo...
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China Food Publishing Company
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ftdoajarticles:oai:doaj.org/article:1599a741369f4bd4ac176ed5b4901387 2024-09-15T17:48:44+00:00 Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans JIAN Peiyi FENG Mingxi CHEN Rong TANG Lei ZHENG Jinluan ZHAO Lichao 2024-08-01T00:00:00Z https://doi.org/10.7506/spkx1002-6630-20231124-199 https://doaj.org/article/1599a741369f4bd4ac176ed5b4901387 EN ZH eng chi China Food Publishing Company https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-15-013.pdf https://doaj.org/toc/1002-6630 1002-6630 doi:10.7506/spkx1002-6630-20231124-199 https://doaj.org/article/1599a741369f4bd4ac176ed5b4901387 Shipin Kexue, Vol 45, Iss 15, Pp 118-126 (2024) antarctic krill phospholipids caenorhabditis elegans antioxidant anti-aging Food processing and manufacture TP368-456 article 2024 ftdoajarticles https://doi.org/10.7506/spkx1002-6630-20231124-199 2024-08-26T15:21:17Z In order to explore the antioxidant and anti-aging effects and underlying mechanism of Antarctic krill phospholipids, the antioxidant capacity in vitro of krill phospholipids was evaluated by radical scavenging capacities against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation, and ferric ion reducing power. Meanwhile, the effect of krill phospholipids at different concentrations on the life span, swallowing frequency, head swinging frequency, acute oxidative stress resistance, heat stress resistance, reactive oxygen species (ROS) levels, antioxidant capacity and aging-related gene expression in the model organism Caenorhabditis elegans was studied. The results showed that krill phospholipids had good antioxidant capacity in vitro, which was superior to that of soybean phospholipids. Compared with the control group, krill phospholipids at all concentrations tested prolonged the life span of C. elegans, improved the swallowing frequency and mobility, and enhanced the resistance to stress. Moreover, krill phospholipids at the medium and high concentrations significantly increased the activities of superoxide dismutase and catalase (P < 0.01) and the content of glutathione (P < 0.05), and significantly reduced the content of malondialdehyde (MDA) and ROS levels (P < 0.01). In addition, krill phospholipids prolonged the life span of C. elegans by activating the transcription factors DAF-16, SKN-1 and HSF-1 and up-regulating the expression of antioxidant and detoxification genes. To sum up, krill phospholipids have good antioxidant capacity both in vivo and in vitro and delay the aging of C. elegans, thus being a potential functional food ingredient. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Chinese |
topic |
antarctic krill phospholipids caenorhabditis elegans antioxidant anti-aging Food processing and manufacture TP368-456 |
spellingShingle |
antarctic krill phospholipids caenorhabditis elegans antioxidant anti-aging Food processing and manufacture TP368-456 JIAN Peiyi FENG Mingxi CHEN Rong TANG Lei ZHENG Jinluan ZHAO Lichao Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
topic_facet |
antarctic krill phospholipids caenorhabditis elegans antioxidant anti-aging Food processing and manufacture TP368-456 |
description |
In order to explore the antioxidant and anti-aging effects and underlying mechanism of Antarctic krill phospholipids, the antioxidant capacity in vitro of krill phospholipids was evaluated by radical scavenging capacities against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation, and ferric ion reducing power. Meanwhile, the effect of krill phospholipids at different concentrations on the life span, swallowing frequency, head swinging frequency, acute oxidative stress resistance, heat stress resistance, reactive oxygen species (ROS) levels, antioxidant capacity and aging-related gene expression in the model organism Caenorhabditis elegans was studied. The results showed that krill phospholipids had good antioxidant capacity in vitro, which was superior to that of soybean phospholipids. Compared with the control group, krill phospholipids at all concentrations tested prolonged the life span of C. elegans, improved the swallowing frequency and mobility, and enhanced the resistance to stress. Moreover, krill phospholipids at the medium and high concentrations significantly increased the activities of superoxide dismutase and catalase (P < 0.01) and the content of glutathione (P < 0.05), and significantly reduced the content of malondialdehyde (MDA) and ROS levels (P < 0.01). In addition, krill phospholipids prolonged the life span of C. elegans by activating the transcription factors DAF-16, SKN-1 and HSF-1 and up-regulating the expression of antioxidant and detoxification genes. To sum up, krill phospholipids have good antioxidant capacity both in vivo and in vitro and delay the aging of C. elegans, thus being a potential functional food ingredient. |
format |
Article in Journal/Newspaper |
author |
JIAN Peiyi FENG Mingxi CHEN Rong TANG Lei ZHENG Jinluan ZHAO Lichao |
author_facet |
JIAN Peiyi FENG Mingxi CHEN Rong TANG Lei ZHENG Jinluan ZHAO Lichao |
author_sort |
JIAN Peiyi |
title |
Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
title_short |
Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
title_full |
Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
title_fullStr |
Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
title_full_unstemmed |
Antioxidant Activity in Vitro of Antarctic Krill Phospholipids and Its Anti-aging Effect on Caenorhabditis elegans |
title_sort |
antioxidant activity in vitro of antarctic krill phospholipids and its anti-aging effect on caenorhabditis elegans |
publisher |
China Food Publishing Company |
publishDate |
2024 |
url |
https://doi.org/10.7506/spkx1002-6630-20231124-199 https://doaj.org/article/1599a741369f4bd4ac176ed5b4901387 |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Shipin Kexue, Vol 45, Iss 15, Pp 118-126 (2024) |
op_relation |
https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-15-013.pdf https://doaj.org/toc/1002-6630 1002-6630 doi:10.7506/spkx1002-6630-20231124-199 https://doaj.org/article/1599a741369f4bd4ac176ed5b4901387 |
op_doi |
https://doi.org/10.7506/spkx1002-6630-20231124-199 |
_version_ |
1810290239058477056 |