Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families.
BACKGROUND:Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas' disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two s...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005313 https://doaj.org/article/1594643502354eb68494ff4ca8c405aa |
id |
ftdoajarticles:oai:doaj.org/article:1594643502354eb68494ff4ca8c405aa |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:1594643502354eb68494ff4ca8c405aa 2023-05-15T15:12:17+02:00 Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. Lucila Traverso Andrés Lavore Ivana Sierra Victorio Palacio Jesús Martinez-Barnetche José Manuel Latorre-Estivalis Gaston Mougabure-Cueto Flavio Francini Marcelo G Lorenzo Mario Henry Rodríguez Sheila Ons Rolando V Rivera-Pomar 2017-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005313 https://doaj.org/article/1594643502354eb68494ff4ca8c405aa EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5310753?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005313 https://doaj.org/article/1594643502354eb68494ff4ca8c405aa PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005313 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005313 2022-12-31T08:43:06Z BACKGROUND:Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas' disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. Therefore, the incorporation of strategies for resistance management is a main concern for vector control programs. Three enzymatic superfamilies are thought to mediate xenobiotic detoxification and resistance: Glutathione Transferases (GSTs), Cytochromes P450 (CYPs) and Carboxyl/Cholinesterases (CCEs). Improving our knowledge of key triatomine detoxification enzymes will strengthen our understanding of insecticide resistance processes in vectors of Chagas' disease. METHODS AND FINDINGS:The discovery and description of detoxification gene superfamilies in normalized transcriptomes of three triatomine species: Triatoma dimidiata, Triatoma infestans and Triatoma pallidipennis is presented. Furthermore, a comparative analysis of these superfamilies among the triatomine transcriptomes and the genome of Rhodnius prolixus, also a triatomine vector of Chagas' disease, and other well-studied insect genomes was performed. The expression pattern of detoxification genes in R. prolixus transcriptomes from key organs was analyzed. The comparisons reveal gene expansions in Sigma class GSTs, CYP3 in CYP superfamily and clade E in CCE superfamily. Moreover, several CYP families identified in these triatomines have not yet been described in other insects. Conversely, several groups of insecticide resistance related enzymes within each enzyme superfamily are reduced or lacking in triatomines. Furthermore, our qRT-PCR results showed an increase in the expression of a CYP4 gene in a T. infestans population resistant to pyrethroids. These results could point to an involvement of metabolic detoxification mechanisms on the high levels of pyrethroid ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 2 e0005313 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lucila Traverso Andrés Lavore Ivana Sierra Victorio Palacio Jesús Martinez-Barnetche José Manuel Latorre-Estivalis Gaston Mougabure-Cueto Flavio Francini Marcelo G Lorenzo Mario Henry Rodríguez Sheila Ons Rolando V Rivera-Pomar Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas' disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. Therefore, the incorporation of strategies for resistance management is a main concern for vector control programs. Three enzymatic superfamilies are thought to mediate xenobiotic detoxification and resistance: Glutathione Transferases (GSTs), Cytochromes P450 (CYPs) and Carboxyl/Cholinesterases (CCEs). Improving our knowledge of key triatomine detoxification enzymes will strengthen our understanding of insecticide resistance processes in vectors of Chagas' disease. METHODS AND FINDINGS:The discovery and description of detoxification gene superfamilies in normalized transcriptomes of three triatomine species: Triatoma dimidiata, Triatoma infestans and Triatoma pallidipennis is presented. Furthermore, a comparative analysis of these superfamilies among the triatomine transcriptomes and the genome of Rhodnius prolixus, also a triatomine vector of Chagas' disease, and other well-studied insect genomes was performed. The expression pattern of detoxification genes in R. prolixus transcriptomes from key organs was analyzed. The comparisons reveal gene expansions in Sigma class GSTs, CYP3 in CYP superfamily and clade E in CCE superfamily. Moreover, several CYP families identified in these triatomines have not yet been described in other insects. Conversely, several groups of insecticide resistance related enzymes within each enzyme superfamily are reduced or lacking in triatomines. Furthermore, our qRT-PCR results showed an increase in the expression of a CYP4 gene in a T. infestans population resistant to pyrethroids. These results could point to an involvement of metabolic detoxification mechanisms on the high levels of pyrethroid ... |
format |
Article in Journal/Newspaper |
author |
Lucila Traverso Andrés Lavore Ivana Sierra Victorio Palacio Jesús Martinez-Barnetche José Manuel Latorre-Estivalis Gaston Mougabure-Cueto Flavio Francini Marcelo G Lorenzo Mario Henry Rodríguez Sheila Ons Rolando V Rivera-Pomar |
author_facet |
Lucila Traverso Andrés Lavore Ivana Sierra Victorio Palacio Jesús Martinez-Barnetche José Manuel Latorre-Estivalis Gaston Mougabure-Cueto Flavio Francini Marcelo G Lorenzo Mario Henry Rodríguez Sheila Ons Rolando V Rivera-Pomar |
author_sort |
Lucila Traverso |
title |
Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
title_short |
Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
title_full |
Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
title_fullStr |
Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
title_full_unstemmed |
Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
title_sort |
comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005313 https://doaj.org/article/1594643502354eb68494ff4ca8c405aa |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005313 (2017) |
op_relation |
http://europepmc.org/articles/PMC5310753?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005313 https://doaj.org/article/1594643502354eb68494ff4ca8c405aa |
op_doi |
https://doi.org/10.1371/journal.pntd.0005313 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
2 |
container_start_page |
e0005313 |
_version_ |
1766342994542198784 |