Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia
Abstract Background The Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) is an important P. falciparum merozoite ligand that mediates invasion of erythrocytes by interacting with a chymotrypsin-sensitive “receptor Z”. A large deletion polymorphism is found in the c-terminal ect...
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ftdoajarticles:oai:doaj.org/article:15740949c3a544c7aebc6376a89abadf 2023-05-15T15:18:12+02:00 Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia Cyrille K. Diédhiou Rahama A. Moussa Amy K. Bei Rachel Daniels Nasserdine Papa Mze Daouda Ndiaye Ngor Faye Dyann Wirth Alfred Amambua-Ngwa Souleymane Mboup Ambroise D. Ahouidi 2019-07-01T00:00:00Z https://doi.org/10.1186/s12936-019-2868-x https://doaj.org/article/15740949c3a544c7aebc6376a89abadf EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2868-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2868-x 1475-2875 https://doaj.org/article/15740949c3a544c7aebc6376a89abadf Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) Plasmodium falciparum PfRh2b SNPs Senegal Gambia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2868-x 2022-12-31T15:57:24Z Abstract Background The Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) is an important P. falciparum merozoite ligand that mediates invasion of erythrocytes by interacting with a chymotrypsin-sensitive “receptor Z”. A large deletion polymorphism is found in the c-terminal ectodomain of this protein in many countries around the world, resulting in a truncated, but expressed protein. The varying frequencies by region suggest that there could be region specific immune selection at this locus. Therefore, this study was designed to determine temporal changes in the PfRh2b deletion polymorphism in infected individuals from Thiès (Senegal) and Western Gambia (The Gambia). It was also sought to determine the selective pressures acting at this locus and whether prevalence of the deletion in isolates genotyped by a 24-SNP molecular barcode is linked to background genotype or whether there might be independent selection acting at this locus. Methods Infected blood samples were sourced from archives of previous studies conducted between 2007 and 2013 at SLAP clinic in Thiès and from 1984 to 2013 in Western Gambia by MRC Unit at LSHTM, The Gambia. A total of 1380 samples were screened for the dimorphic alleles of the PfRh2b using semi-nested Polymerase Chain Reaction PCR. Samples from Thiès were previously barcoded. Results In Thiès, a consistent trend of decreasing prevalence of the PfRh2b deletion over time was observed: from 66.54% in 2007 and to 38.1% in 2013. In contrast, in Western Gambia, the frequency of the deletion fluctuated over time; it increased between 1984 and 2005 from (58.04%) to (69.33%) and decreased to 47.47% in 2007. Between 2007 and 2012, the prevalence of this deletion increased significantly from 47.47 to 83.02% and finally declined significantly to 57.94% in 2013. Association between the presence of this deletion and age was found in Thiès, however, not in Western Gambia. For the majority of isolates, the PfRh2b alleles could be tracked with specific 24-SNP barcoded genotype, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Plasmodium falciparum PfRh2b SNPs Senegal Gambia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum PfRh2b SNPs Senegal Gambia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Cyrille K. Diédhiou Rahama A. Moussa Amy K. Bei Rachel Daniels Nasserdine Papa Mze Daouda Ndiaye Ngor Faye Dyann Wirth Alfred Amambua-Ngwa Souleymane Mboup Ambroise D. Ahouidi Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
topic_facet |
Plasmodium falciparum PfRh2b SNPs Senegal Gambia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) is an important P. falciparum merozoite ligand that mediates invasion of erythrocytes by interacting with a chymotrypsin-sensitive “receptor Z”. A large deletion polymorphism is found in the c-terminal ectodomain of this protein in many countries around the world, resulting in a truncated, but expressed protein. The varying frequencies by region suggest that there could be region specific immune selection at this locus. Therefore, this study was designed to determine temporal changes in the PfRh2b deletion polymorphism in infected individuals from Thiès (Senegal) and Western Gambia (The Gambia). It was also sought to determine the selective pressures acting at this locus and whether prevalence of the deletion in isolates genotyped by a 24-SNP molecular barcode is linked to background genotype or whether there might be independent selection acting at this locus. Methods Infected blood samples were sourced from archives of previous studies conducted between 2007 and 2013 at SLAP clinic in Thiès and from 1984 to 2013 in Western Gambia by MRC Unit at LSHTM, The Gambia. A total of 1380 samples were screened for the dimorphic alleles of the PfRh2b using semi-nested Polymerase Chain Reaction PCR. Samples from Thiès were previously barcoded. Results In Thiès, a consistent trend of decreasing prevalence of the PfRh2b deletion over time was observed: from 66.54% in 2007 and to 38.1% in 2013. In contrast, in Western Gambia, the frequency of the deletion fluctuated over time; it increased between 1984 and 2005 from (58.04%) to (69.33%) and decreased to 47.47% in 2007. Between 2007 and 2012, the prevalence of this deletion increased significantly from 47.47 to 83.02% and finally declined significantly to 57.94% in 2013. Association between the presence of this deletion and age was found in Thiès, however, not in Western Gambia. For the majority of isolates, the PfRh2b alleles could be tracked with specific 24-SNP barcoded genotype, ... |
format |
Article in Journal/Newspaper |
author |
Cyrille K. Diédhiou Rahama A. Moussa Amy K. Bei Rachel Daniels Nasserdine Papa Mze Daouda Ndiaye Ngor Faye Dyann Wirth Alfred Amambua-Ngwa Souleymane Mboup Ambroise D. Ahouidi |
author_facet |
Cyrille K. Diédhiou Rahama A. Moussa Amy K. Bei Rachel Daniels Nasserdine Papa Mze Daouda Ndiaye Ngor Faye Dyann Wirth Alfred Amambua-Ngwa Souleymane Mboup Ambroise D. Ahouidi |
author_sort |
Cyrille K. Diédhiou |
title |
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
title_short |
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
title_full |
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
title_fullStr |
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
title_full_unstemmed |
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia |
title_sort |
temporal changes in plasmodium falciparum reticulocyte binding protein homolog 2b (pfrh2b) in senegal and the gambia |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2868-x https://doaj.org/article/15740949c3a544c7aebc6376a89abadf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2868-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2868-x 1475-2875 https://doaj.org/article/15740949c3a544c7aebc6376a89abadf |
op_doi |
https://doi.org/10.1186/s12936-019-2868-x |
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Malaria Journal |
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18 |
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1766348417884225536 |