Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunos...
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ftdoajarticles:oai:doaj.org/article:1567ae515d444bc0a436d6df07f00043 2023-10-01T03:59:07+02:00 Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz 2003-07-01T00:00:00Z https://doi.org/10.1590/S0074-02762003000500017 https://doaj.org/article/1567ae515d444bc0a436d6df07f00043 EN eng Fundação Oswaldo Cruz (FIOCRUZ) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500017 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762003000500017 0074-0276 1678-8060 https://doaj.org/article/1567ae515d444bc0a436d6df07f00043 Memorias do Instituto Oswaldo Cruz, Vol 98, Iss 5, Pp 687-691 (2003) Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting Microbiology QR1-502 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1590/S0074-02762003000500017 2023-09-03T00:35:45Z The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Memórias do Instituto Oswaldo Cruz 98 5 687 691 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting Microbiology QR1-502 Infectious and parasitic diseases RC109-216 Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
topic_facet |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
description |
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. |
format |
Article in Journal/Newspaper |
author |
Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz |
author_facet |
Luciana Pereira Silva Ivanildes Solange da Costa Barcelos Andréia Barcelos Passos-Lima Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-Cruz |
author_sort |
Luciana Pereira Silva |
title |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_short |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_full |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_fullStr |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_full_unstemmed |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_sort |
western blotting using strongyloides ratti antigen for the detection of igg antibodies as confirmatory test in human strongyloidiasis |
publisher |
Fundação Oswaldo Cruz (FIOCRUZ) |
publishDate |
2003 |
url |
https://doi.org/10.1590/S0074-02762003000500017 https://doaj.org/article/1567ae515d444bc0a436d6df07f00043 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Memorias do Instituto Oswaldo Cruz, Vol 98, Iss 5, Pp 687-691 (2003) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500017 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762003000500017 0074-0276 1678-8060 https://doaj.org/article/1567ae515d444bc0a436d6df07f00043 |
op_doi |
https://doi.org/10.1590/S0074-02762003000500017 |
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Memórias do Instituto Oswaldo Cruz |
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98 |
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