Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species
Objective: To investigate the antifungal activity of the fern species Lygodium venustum (L. venustum) and Pityrogramma calomelanos (P. calomelanos) against Candida albicans and Candida tropicalis strains. Methods: The microdilution method was used to evaluate the antifungal activity, as well as the...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2017.09.018 https://doaj.org/article/15672bef17aa48638454339b0fac214c |
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ftdoajarticles:oai:doaj.org/article:15672bef17aa48638454339b0fac214c 2023-05-15T15:09:30+02:00 Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species Maria A. Freitas Antonia T.L. Santos Antonio J.T. Machado Ana Raquel P. Silva Fábia F. Campina Maria S. Costa Gioconda M.A.B. Martins Maria Flaviana B. Morais-Braga Saulo R. Tintino Irwin R.A. Menezes Jaime Ribeiro-Filho Altevir P. Medeiros Adeliana S. Oliveira Patrício B. Maracajá Henrique D.M. Coutinho 2017-11-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.09.018 https://doaj.org/article/15672bef17aa48638454339b0fac214c EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117310304 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.09.018 https://doaj.org/article/15672bef17aa48638454339b0fac214c Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 11, Pp 1025-1030 (2017) Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.09.018 2022-12-30T22:11:05Z Objective: To investigate the antifungal activity of the fern species Lygodium venustum (L. venustum) and Pityrogramma calomelanos (P. calomelanos) against Candida albicans and Candida tropicalis strains. Methods: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration (MIC), minimum fungicide concentration and morphological changes were also determined. Results: The extract obtained from L. venustum presented a MIC > 8192 μg/mL, while the extract obtained from and P. calomelanos presented a MIC = 8192 μg/mL, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the fungi. Conclusions: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 11 1025 1030 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maria A. Freitas Antonia T.L. Santos Antonio J.T. Machado Ana Raquel P. Silva Fábia F. Campina Maria S. Costa Gioconda M.A.B. Martins Maria Flaviana B. Morais-Braga Saulo R. Tintino Irwin R.A. Menezes Jaime Ribeiro-Filho Altevir P. Medeiros Adeliana S. Oliveira Patrício B. Maracajá Henrique D.M. Coutinho Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
topic_facet |
Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antifungal activity of the fern species Lygodium venustum (L. venustum) and Pityrogramma calomelanos (P. calomelanos) against Candida albicans and Candida tropicalis strains. Methods: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration (MIC), minimum fungicide concentration and morphological changes were also determined. Results: The extract obtained from L. venustum presented a MIC > 8192 μg/mL, while the extract obtained from and P. calomelanos presented a MIC = 8192 μg/mL, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the fungi. Conclusions: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections. |
format |
Article in Journal/Newspaper |
author |
Maria A. Freitas Antonia T.L. Santos Antonio J.T. Machado Ana Raquel P. Silva Fábia F. Campina Maria S. Costa Gioconda M.A.B. Martins Maria Flaviana B. Morais-Braga Saulo R. Tintino Irwin R.A. Menezes Jaime Ribeiro-Filho Altevir P. Medeiros Adeliana S. Oliveira Patrício B. Maracajá Henrique D.M. Coutinho |
author_facet |
Maria A. Freitas Antonia T.L. Santos Antonio J.T. Machado Ana Raquel P. Silva Fábia F. Campina Maria S. Costa Gioconda M.A.B. Martins Maria Flaviana B. Morais-Braga Saulo R. Tintino Irwin R.A. Menezes Jaime Ribeiro-Filho Altevir P. Medeiros Adeliana S. Oliveira Patrício B. Maracajá Henrique D.M. Coutinho |
author_sort |
Maria A. Freitas |
title |
Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
title_short |
Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
title_full |
Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
title_fullStr |
Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
title_full_unstemmed |
Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species |
title_sort |
fern extracts potentiate fluconazole activity and inhibit morphological changes in candida species |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.09.018 https://doaj.org/article/15672bef17aa48638454339b0fac214c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 11, Pp 1025-1030 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117310304 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.09.018 https://doaj.org/article/15672bef17aa48638454339b0fac214c |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.09.018 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
11 |
container_start_page |
1025 |
op_container_end_page |
1030 |
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1766340681988571136 |