Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique.
World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if str...
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ftdoajarticles:oai:doaj.org/article:152b933e7c234cd5baa286a7395c1dbe 2023-05-15T15:15:27+02:00 Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. Berta Grau-Pujol Helena Martí-Soler Valdemiro Escola Maria Demontis Jose Carlos Jamine Javier Gandasegui Osvaldo Muchisse Maria Cambra-Pellejà Anelsio Cossa Maria Martinez-Valladares Charfudin Sacoor Lisette Van Lieshout Jorge Cano Emanuele Giorgi Jose Muñoz 2021-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009803 https://doaj.org/article/152b933e7c234cd5baa286a7395c1dbe EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009803 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009803 https://doaj.org/article/152b933e7c234cd5baa286a7395c1dbe PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009803 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009803 2022-12-31T07:37:33Z World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%- 97%) for A. lumbricoides, 95% (CI: 88%- 98%) for T. trichiura and 95% (CI: 91%- 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 10 e0009803 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Berta Grau-Pujol Helena Martí-Soler Valdemiro Escola Maria Demontis Jose Carlos Jamine Javier Gandasegui Osvaldo Muchisse Maria Cambra-Pellejà Anelsio Cossa Maria Martinez-Valladares Charfudin Sacoor Lisette Van Lieshout Jorge Cano Emanuele Giorgi Jose Muñoz Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%- 97%) for A. lumbricoides, 95% (CI: 88%- 98%) for T. trichiura and 95% (CI: 91%- 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate ... |
format |
Article in Journal/Newspaper |
author |
Berta Grau-Pujol Helena Martí-Soler Valdemiro Escola Maria Demontis Jose Carlos Jamine Javier Gandasegui Osvaldo Muchisse Maria Cambra-Pellejà Anelsio Cossa Maria Martinez-Valladares Charfudin Sacoor Lisette Van Lieshout Jorge Cano Emanuele Giorgi Jose Muñoz |
author_facet |
Berta Grau-Pujol Helena Martí-Soler Valdemiro Escola Maria Demontis Jose Carlos Jamine Javier Gandasegui Osvaldo Muchisse Maria Cambra-Pellejà Anelsio Cossa Maria Martinez-Valladares Charfudin Sacoor Lisette Van Lieshout Jorge Cano Emanuele Giorgi Jose Muñoz |
author_sort |
Berta Grau-Pujol |
title |
Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
title_short |
Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
title_full |
Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
title_fullStr |
Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
title_full_unstemmed |
Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. |
title_sort |
towards soil-transmitted helminths transmission interruption: the impact of diagnostic tools on infection prediction in a low intensity setting in southern mozambique. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009803 https://doaj.org/article/152b933e7c234cd5baa286a7395c1dbe |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009803 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009803 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009803 https://doaj.org/article/152b933e7c234cd5baa286a7395c1dbe |
op_doi |
https://doi.org/10.1371/journal.pntd.0009803 |
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PLOS Neglected Tropical Diseases |
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15 |
container_issue |
10 |
container_start_page |
e0009803 |
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1766345821801938944 |