In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia
Introduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates. O...
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ftdoajarticles:oai:doaj.org/article:14da65a9637445ff877dd66b3dda1694 2023-05-15T15:12:50+02:00 In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez 2018-08-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i3.3408 https://doaj.org/article/14da65a9637445ff877dd66b3dda1694 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3408 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i3.3408 https://doaj.org/article/14da65a9637445ff877dd66b3dda1694 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 0, Pp 24-29 (2018) Nematoda snails culture media in vitro techniques Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i3.3408 2022-12-30T23:57:09Z Introduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates. Objective: To standardize culture in vitro of L3 carried by L. fulica from Santa Fe de Antioquia. Materials and methods: Between July and November, 2014, 10 snails were collected, killed, and conserved with HCl 0.7%. Larvae were recovered using the Baermann technique and cultured for 36 days in Schneider, DMEM and RPMI media, with and without SFB 20% and distilled water with SFB 20%. Replacements were made every 36 hours; larvae were measured with an ocular micrometer on a microscope. Summary statistics were estimated; box and whisker plots were made; the t Student test was performed in SPSS 18™. A p-value below 0.05 was assumed as significant. Results: Fifty per cent of the larvae survived. The highest survival and growth was 85% in supplemented DMEM. The final average length of larvae in supplemented media exceeded the initial one. There were significant differences between the average length of larvae cultured in supplemented media and the initial length. The initial width of larvae did not change. Conclusions: The best medium for the culture of L3 larvae was supplemented DMEM. The length provided more information than the width for the larval growth evaluation. The larvae studied did not correspond to Angiostrongylus cantonensis, A. costaricensis or Aelurostrongylus abstrusus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 38 24 29 |
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English Spanish |
topic |
Nematoda snails culture media in vitro techniques Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Nematoda snails culture media in vitro techniques Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
topic_facet |
Nematoda snails culture media in vitro techniques Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates. Objective: To standardize culture in vitro of L3 carried by L. fulica from Santa Fe de Antioquia. Materials and methods: Between July and November, 2014, 10 snails were collected, killed, and conserved with HCl 0.7%. Larvae were recovered using the Baermann technique and cultured for 36 days in Schneider, DMEM and RPMI media, with and without SFB 20% and distilled water with SFB 20%. Replacements were made every 36 hours; larvae were measured with an ocular micrometer on a microscope. Summary statistics were estimated; box and whisker plots were made; the t Student test was performed in SPSS 18™. A p-value below 0.05 was assumed as significant. Results: Fifty per cent of the larvae survived. The highest survival and growth was 85% in supplemented DMEM. The final average length of larvae in supplemented media exceeded the initial one. There were significant differences between the average length of larvae cultured in supplemented media and the initial length. The initial width of larvae did not change. Conclusions: The best medium for the culture of L3 larvae was supplemented DMEM. The length provided more information than the width for the larval growth evaluation. The larvae studied did not correspond to Angiostrongylus cantonensis, A. costaricensis or Aelurostrongylus abstrusus. |
format |
Article in Journal/Newspaper |
author |
Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez |
author_facet |
Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez |
author_sort |
Nelly Solfania Heredia |
title |
In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
title_short |
In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
title_full |
In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
title_fullStr |
In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
title_full_unstemmed |
In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
title_sort |
in vitro culture of l3 larvae of nematodes obtained from the african giant snail lissachatina fulica (mollusca: gastropoda) in santa fe de antioquia |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i3.3408 https://doaj.org/article/14da65a9637445ff877dd66b3dda1694 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 0, Pp 24-29 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3408 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i3.3408 https://doaj.org/article/14da65a9637445ff877dd66b3dda1694 |
op_doi |
https://doi.org/10.7705/biomedica.v38i3.3408 |
container_title |
Biomédica |
container_volume |
38 |
container_start_page |
24 |
op_container_end_page |
29 |
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1766343465993502720 |