Calculation of United Quality Latent Indices of Deschampsia antarctica plants adaptability of different origin grown in vitro

• Published in: Ukrainian Antarctic Journal
• Main Authors: N. Miryuta, I. Parnikoza, O. Poronnik, G. Myryuta, M. Rojek-Jelonek, E. Dykyi, V. Kunakh
• Format: Article in Journal/Newspaper
• Language: English; Ukrainian
• Published: State Institution National Antarctic Scientific Center 2021
• Subjects: deschampsia antarctica; in vitro plant culture; the united quality latent index of plant adaptability; probabilistic relation correlation models of different indices; Meteorology. Climatology; QC851-999; Geophysics. Cosmic physics; QC801-809; Antarctic; Argentine; Argentine Islands; Antarc*; Antarctica
• Online Access: https://doi.org/10.33275/1727-7485.1.2021.667; https://doaj.org/article/14c94dd5bc084ffca0546cab4477b729

The research was to develop and describe in detail the algorithm for calculating the United Quality Latent Index (UQLI, Iqi) of plant adaptability from the collection of Deschampsia antarctica Ė. Desv. genotypes obtained from seeds collected at different sites in the Argentine Islands region, the maritime Antarctic, and grown in vitro at the laboratory conditions. Genome size and genetic distances by ISSR and IRAP markers according to data from published articles were used as basic indices of initial genetic heterogeneity for analyzed plant genotypes. To assess individual adaptability indices for eleven D. antarctica genotypes, we used measurement of the leaf length morphometric index and determination of the flavonoids content by rutin and the content of photosynthetic pigments. The spectra of reserve and protective proteins in leaves were investigated by polyacrylamide gel electrophoresis. To obtain the United Quality Latent Index of Adaptability (Iqi, UQLI), the method of extreme grouping was used. The estimation of Iqi (UQLI) was performed using pairwise comparisons of indices from differences sets for each pair of genotypes. We developed and described in detail the algorithm for Iqi estimation for eleven D. antarctica genotypes. As an example of application, correlation models of probability relations of the indices are presented. To evaluate the complex adaptability for eleven D. antarctica genotypes grown in vitro we used developed algorithm for the UQLI calculation. The individuality of the adaptive portrait for all studied genotypes under in vitro cultivation conditions was shown. The influence of basic genetic characteristics (genome size and genetic distances) on auxin metabolism-related indices of leaf length and flavonoid content was shown. Such effect may be carried out by genetic characteristics both individually and together, probably via auxin metabolism. Among the eight genotypes researched, we distinguish four different variants by correlation models and two (positive and negative) by the ...