Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x106 CFU/fish) but no to bath exposure. The i.p. c...
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ftdoajarticles:oai:doaj.org/article:14899877b9ae45c5acf2f0d56bc4a46a 2023-05-15T18:41:06+02:00 Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study JL Romalde AE Toranzo J Lamas B Magariños S Lopez-Romalde GE Magi 2009-06-01T00:00:00Z https://doi.org/10.4081/ejh.2009.73 https://doaj.org/article/14899877b9ae45c5acf2f0d56bc4a46a EN eng PAGEPress Publications http://ejh.pagepress.org/index.php/ejh/article/view/1235 https://doaj.org/toc/1121-760X doi:10.4081/ejh.2009.73 1121-760X https://doaj.org/article/14899877b9ae45c5acf2f0d56bc4a46a European Journal of Histochemistry, Vol 53, Iss 2, Pp 73-80 (2009) Biology (General) QH301-705.5 article 2009 ftdoajarticles https://doi.org/10.4081/ejh.2009.73 2022-12-31T08:49:23Z Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x106 CFU/fish) but no to bath exposure. The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles European Journal of Histochemistry 53 2 |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 JL Romalde AE Toranzo J Lamas B Magariños S Lopez-Romalde GE Magi Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
topic_facet |
Biology (General) QH301-705.5 |
description |
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x106 CFU/fish) but no to bath exposure. The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. |
format |
Article in Journal/Newspaper |
author |
JL Romalde AE Toranzo J Lamas B Magariños S Lopez-Romalde GE Magi |
author_facet |
JL Romalde AE Toranzo J Lamas B Magariños S Lopez-Romalde GE Magi |
author_sort |
JL Romalde |
title |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_short |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_fullStr |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full_unstemmed |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_sort |
experimental pseudomonas anguilliseptica infection in turbot psetta maxima (l.): a histopathological and immunohistochemical study |
publisher |
PAGEPress Publications |
publishDate |
2009 |
url |
https://doi.org/10.4081/ejh.2009.73 https://doaj.org/article/14899877b9ae45c5acf2f0d56bc4a46a |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
European Journal of Histochemistry, Vol 53, Iss 2, Pp 73-80 (2009) |
op_relation |
http://ejh.pagepress.org/index.php/ejh/article/view/1235 https://doaj.org/toc/1121-760X doi:10.4081/ejh.2009.73 1121-760X https://doaj.org/article/14899877b9ae45c5acf2f0d56bc4a46a |
op_doi |
https://doi.org/10.4081/ejh.2009.73 |
container_title |
European Journal of Histochemistry |
container_volume |
53 |
container_issue |
2 |
_version_ |
1766230601483943936 |