First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core
Abstract Despite the unique physiology and metabolic pathways of microbiomes from cold environments providing key evolutionary insights and promising leads for discovering new bioactive compounds, cultivable bacteria entrapped in perennial ice from caves remained a largely unexplored life system. In...
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ftdoajarticles:oai:doaj.org/article:147a919157774f43b47a86db61d704c5 2023-05-15T16:38:55+02:00 First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core Victoria I. Paun Paris Lavin Mariana C. Chifiriuc Cristina Purcarea 2021-01-01T00:00:00Z https://doi.org/10.1038/s41598-020-79754-5 https://doaj.org/article/147a919157774f43b47a86db61d704c5 EN eng Nature Portfolio https://doi.org/10.1038/s41598-020-79754-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-79754-5 2045-2322 https://doaj.org/article/147a919157774f43b47a86db61d704c5 Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1038/s41598-020-79754-5 2022-12-31T08:07:20Z Abstract Despite the unique physiology and metabolic pathways of microbiomes from cold environments providing key evolutionary insights and promising leads for discovering new bioactive compounds, cultivable bacteria entrapped in perennial ice from caves remained a largely unexplored life system. In this context, we obtained and characterized bacterial strains from 13,000-years old ice core of Scarisoara Ice Cave, providing first isolates from perennial ice accumulated in caves since Late Glacial, and first culture-based evidences of bacterial resistome and antimicrobial compounds production. The 68 bacterial isolates belonged to 4 phyla, 34 genera and 56 species, with 17 strains representing putative new taxa. The Gram-negative cave bacteria (Proteobacteria and Bacteroidetes) were more resistant to the great majority of antibiotic classes than the Gram-positive ones (Actinobacteria, Firmicutes). More than 50% of the strains exhibited high resistance to 17 classes of antibiotics. Some of the isolates inhibited the growth of clinically important Gram-positive and Gram-negative resistant strains and revealed metabolic features with applicative potential. The current report on bacterial strains from millennia-old cave ice revealed promising candidates for studying the evolution of environmental resistome and for obtaining new active biomolecules for fighting the antibiotics crisis, and valuable cold-active biocatalysts. Article in Journal/Newspaper ice core Directory of Open Access Journals: DOAJ Articles Scientific Reports 11 1 |
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Medicine R Science Q |
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Medicine R Science Q Victoria I. Paun Paris Lavin Mariana C. Chifiriuc Cristina Purcarea First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
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Medicine R Science Q |
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Abstract Despite the unique physiology and metabolic pathways of microbiomes from cold environments providing key evolutionary insights and promising leads for discovering new bioactive compounds, cultivable bacteria entrapped in perennial ice from caves remained a largely unexplored life system. In this context, we obtained and characterized bacterial strains from 13,000-years old ice core of Scarisoara Ice Cave, providing first isolates from perennial ice accumulated in caves since Late Glacial, and first culture-based evidences of bacterial resistome and antimicrobial compounds production. The 68 bacterial isolates belonged to 4 phyla, 34 genera and 56 species, with 17 strains representing putative new taxa. The Gram-negative cave bacteria (Proteobacteria and Bacteroidetes) were more resistant to the great majority of antibiotic classes than the Gram-positive ones (Actinobacteria, Firmicutes). More than 50% of the strains exhibited high resistance to 17 classes of antibiotics. Some of the isolates inhibited the growth of clinically important Gram-positive and Gram-negative resistant strains and revealed metabolic features with applicative potential. The current report on bacterial strains from millennia-old cave ice revealed promising candidates for studying the evolution of environmental resistome and for obtaining new active biomolecules for fighting the antibiotics crisis, and valuable cold-active biocatalysts. |
format |
Article in Journal/Newspaper |
author |
Victoria I. Paun Paris Lavin Mariana C. Chifiriuc Cristina Purcarea |
author_facet |
Victoria I. Paun Paris Lavin Mariana C. Chifiriuc Cristina Purcarea |
author_sort |
Victoria I. Paun |
title |
First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
title_short |
First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
title_full |
First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
title_fullStr |
First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
title_full_unstemmed |
First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
title_sort |
first report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41598-020-79754-5 https://doaj.org/article/147a919157774f43b47a86db61d704c5 |
genre |
ice core |
genre_facet |
ice core |
op_source |
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
op_relation |
https://doi.org/10.1038/s41598-020-79754-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-79754-5 2045-2322 https://doaj.org/article/147a919157774f43b47a86db61d704c5 |
op_doi |
https://doi.org/10.1038/s41598-020-79754-5 |
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Scientific Reports |
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11 |
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1 |
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1766029276865363968 |