Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR.
Hydatidosis or echinococcosisis considered a neglected zoonotic disease despite its high burden in the livestock industry and the high risk of infection by humans in endemic areas. In a cross-sectional study we estimated the copro-Incidence and also genotyped Echinococcus granulosus isolates from do...
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ftdoajarticles:oai:doaj.org/article:1447b873c51f4916bc435ae86e12273a 2023-05-15T15:10:36+02:00 Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. Amer Al-Jawabreh Kamal Dumaidi Suheir Ereqat Abedelmajeed Nasereddin Hanan Al-Jawabreh Kifaya Azmi Nahed Al-Laham Ziad Abdeen 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003934 https://doaj.org/article/1447b873c51f4916bc435ae86e12273a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4504717?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003934 https://doaj.org/article/1447b873c51f4916bc435ae86e12273a PLoS Neglected Tropical Diseases, Vol 9, Iss 7, p e0003934 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003934 2022-12-31T16:25:01Z Hydatidosis or echinococcosisis considered a neglected zoonotic disease despite its high burden in the livestock industry and the high risk of infection by humans in endemic areas. In a cross-sectional study we estimated the copro-Incidence and also genotyped Echinococcus granulosus isolates from domestic dogs using polymerase chain reaction (PCR). Medical archives in nine major hospitals in Palestine were reviewed to determine incidence of E. granulosus infection detected in humans during surgery. Faecal samples were collected from 93 domestic dogs in three districts with the highest number of human cases: Al-Khalil (Hebron), Tubas and Jenin. Genomic DNA was extracted from dog faecal samples and amplified by PCR targeting the repeat DNA sequence (EgG1 Hae III) followed by sequencing of five positive samples. Genotyping was determined by sequencing and BLAST searching of mitochondrial cytochrome c oxidase subunit (CO1). The incidence of E. granulosus infection detected in humans at surgery was 1.2 per 100,000 in the West Bank and 1.0 per 100,000 in Gaza Strip. Seventeen of 93 domestic dogs (18%) were positive, based upon comparison with the Echinococcus DNA control. The five sequenced samples were confirmed to be E. granulosus. Successfully genotyped sample belonged to E.granulosus sensu stricto (formerly G1-G3 complex, sheep strain). For domestic dogs, age group (13-24 months) and sex were identified as two risk factors for contracting E. granulosus. The study identified the high incidence of E. granulosus sensu stricto in dogs in Palestine. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Hebron ENVELOPE(-62.631,-62.631,58.200,58.200) PLOS Neglected Tropical Diseases 9 7 e0003934 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Amer Al-Jawabreh Kamal Dumaidi Suheir Ereqat Abedelmajeed Nasereddin Hanan Al-Jawabreh Kifaya Azmi Nahed Al-Laham Ziad Abdeen Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Hydatidosis or echinococcosisis considered a neglected zoonotic disease despite its high burden in the livestock industry and the high risk of infection by humans in endemic areas. In a cross-sectional study we estimated the copro-Incidence and also genotyped Echinococcus granulosus isolates from domestic dogs using polymerase chain reaction (PCR). Medical archives in nine major hospitals in Palestine were reviewed to determine incidence of E. granulosus infection detected in humans during surgery. Faecal samples were collected from 93 domestic dogs in three districts with the highest number of human cases: Al-Khalil (Hebron), Tubas and Jenin. Genomic DNA was extracted from dog faecal samples and amplified by PCR targeting the repeat DNA sequence (EgG1 Hae III) followed by sequencing of five positive samples. Genotyping was determined by sequencing and BLAST searching of mitochondrial cytochrome c oxidase subunit (CO1). The incidence of E. granulosus infection detected in humans at surgery was 1.2 per 100,000 in the West Bank and 1.0 per 100,000 in Gaza Strip. Seventeen of 93 domestic dogs (18%) were positive, based upon comparison with the Echinococcus DNA control. The five sequenced samples were confirmed to be E. granulosus. Successfully genotyped sample belonged to E.granulosus sensu stricto (formerly G1-G3 complex, sheep strain). For domestic dogs, age group (13-24 months) and sex were identified as two risk factors for contracting E. granulosus. The study identified the high incidence of E. granulosus sensu stricto in dogs in Palestine. |
format |
Article in Journal/Newspaper |
author |
Amer Al-Jawabreh Kamal Dumaidi Suheir Ereqat Abedelmajeed Nasereddin Hanan Al-Jawabreh Kifaya Azmi Nahed Al-Laham Ziad Abdeen |
author_facet |
Amer Al-Jawabreh Kamal Dumaidi Suheir Ereqat Abedelmajeed Nasereddin Hanan Al-Jawabreh Kifaya Azmi Nahed Al-Laham Ziad Abdeen |
author_sort |
Amer Al-Jawabreh |
title |
Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
title_short |
Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
title_full |
Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
title_fullStr |
Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
title_full_unstemmed |
Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. |
title_sort |
incidence of echinococcus granulosus in domestic dogs in palestine as revealed by copro-pcr. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003934 https://doaj.org/article/1447b873c51f4916bc435ae86e12273a |
long_lat |
ENVELOPE(-62.631,-62.631,58.200,58.200) |
geographic |
Arctic Hebron |
geographic_facet |
Arctic Hebron |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 7, p e0003934 (2015) |
op_relation |
http://europepmc.org/articles/PMC4504717?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003934 https://doaj.org/article/1447b873c51f4916bc435ae86e12273a |
op_doi |
https://doi.org/10.1371/journal.pntd.0003934 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
7 |
container_start_page |
e0003934 |
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1766341601372667904 |