Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach
Polar regions are among the most extreme habitats on Earth. However, diatom biodiversity in those regions is much more extensive and ecologically diverse than previously thought. The objective of this study was to add knowledge to benthic diatom biodiversity in Western Antarctic coastal zones via id...
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ftdoajarticles:oai:doaj.org/article:1441a612a62942cd8f7ab29adbb27de4 2024-01-07T09:39:24+01:00 Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach Katherina Schimani Nélida Abarca Oliver Skibbe Heba Mohamad Regine Jahn Wolf-Henning Kusber Gabriela Laura Campana Jonas Zimmermann 2023-12-01T00:00:00Z https://doi.org/10.3897/mbmg.7.110194 https://doaj.org/article/1441a612a62942cd8f7ab29adbb27de4 EN eng Pensoft Publishers https://mbmg.pensoft.net/article/110194/download/pdf/ https://mbmg.pensoft.net/article/110194/download/xml/ https://mbmg.pensoft.net/article/110194/ https://doaj.org/toc/2534-9708 doi:10.3897/mbmg.7.110194 2534-9708 https://doaj.org/article/1441a612a62942cd8f7ab29adbb27de4 Metabarcoding and Metagenomics, Vol 7, Iss , Pp 339-384 (2023) Ecology QH540-549.5 article 2023 ftdoajarticles https://doi.org/10.3897/mbmg.7.110194 2023-12-10T01:40:17Z Polar regions are among the most extreme habitats on Earth. However, diatom biodiversity in those regions is much more extensive and ecologically diverse than previously thought. The objective of this study was to add knowledge to benthic diatom biodiversity in Western Antarctic coastal zones via identification by means of morphology, DNA metabarcoding and cultured isolates. In addition, a taxonomically validated reference library for Antarctic benthic diatoms was established with comprehensive information on habitat, morphology and DNA barcodes (rbcL and 18SV4). Benthic samples from marine, brackish and freshwater habitats were taken at the Antarctic Peninsula. A total of 162 clonal cultures were established, resulting in the identification of 60 taxa. The combination of total morphological richness of 174 taxa, including the clones, with an additional 73 taxa just assigned by metabarcoding resulted in 247 infrageneric taxa. Of those taxa, 33 were retrieved by all three methods and 111 only by morphology. The barcode reference library of Antarctic species with the new references obtained through culturing allowed the assignment of 47 taxa in the metabarcoding analyses, which would have been left unassigned because no matching reference sequences were available before. Non–metric multidimensional scaling analyses of morphological as well as molecular data showed a clear separation of diatom communities according to water and substratum types. Many species, especially marine taxa, still have no record in reference databases. This highlights the need for a more comprehensive reference library to further improve routine diatom metabarcoding. Overall, a combination of morphological and molecular methods, along with culturing, provides complementary information on the biodiversity of benthic diatoms in the region. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula The Antarctic Metabarcoding and Metagenomics 7 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 |
spellingShingle |
Ecology QH540-549.5 Katherina Schimani Nélida Abarca Oliver Skibbe Heba Mohamad Regine Jahn Wolf-Henning Kusber Gabriela Laura Campana Jonas Zimmermann Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
topic_facet |
Ecology QH540-549.5 |
description |
Polar regions are among the most extreme habitats on Earth. However, diatom biodiversity in those regions is much more extensive and ecologically diverse than previously thought. The objective of this study was to add knowledge to benthic diatom biodiversity in Western Antarctic coastal zones via identification by means of morphology, DNA metabarcoding and cultured isolates. In addition, a taxonomically validated reference library for Antarctic benthic diatoms was established with comprehensive information on habitat, morphology and DNA barcodes (rbcL and 18SV4). Benthic samples from marine, brackish and freshwater habitats were taken at the Antarctic Peninsula. A total of 162 clonal cultures were established, resulting in the identification of 60 taxa. The combination of total morphological richness of 174 taxa, including the clones, with an additional 73 taxa just assigned by metabarcoding resulted in 247 infrageneric taxa. Of those taxa, 33 were retrieved by all three methods and 111 only by morphology. The barcode reference library of Antarctic species with the new references obtained through culturing allowed the assignment of 47 taxa in the metabarcoding analyses, which would have been left unassigned because no matching reference sequences were available before. Non–metric multidimensional scaling analyses of morphological as well as molecular data showed a clear separation of diatom communities according to water and substratum types. Many species, especially marine taxa, still have no record in reference databases. This highlights the need for a more comprehensive reference library to further improve routine diatom metabarcoding. Overall, a combination of morphological and molecular methods, along with culturing, provides complementary information on the biodiversity of benthic diatoms in the region. |
format |
Article in Journal/Newspaper |
author |
Katherina Schimani Nélida Abarca Oliver Skibbe Heba Mohamad Regine Jahn Wolf-Henning Kusber Gabriela Laura Campana Jonas Zimmermann |
author_facet |
Katherina Schimani Nélida Abarca Oliver Skibbe Heba Mohamad Regine Jahn Wolf-Henning Kusber Gabriela Laura Campana Jonas Zimmermann |
author_sort |
Katherina Schimani |
title |
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
title_short |
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
title_full |
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
title_fullStr |
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
title_full_unstemmed |
Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach |
title_sort |
exploring benthic diatom diversity in the west antarctic peninsula: insights from a morphological and molecular approach |
publisher |
Pensoft Publishers |
publishDate |
2023 |
url |
https://doi.org/10.3897/mbmg.7.110194 https://doaj.org/article/1441a612a62942cd8f7ab29adbb27de4 |
geographic |
Antarctic Antarctic Peninsula The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
Metabarcoding and Metagenomics, Vol 7, Iss , Pp 339-384 (2023) |
op_relation |
https://mbmg.pensoft.net/article/110194/download/pdf/ https://mbmg.pensoft.net/article/110194/download/xml/ https://mbmg.pensoft.net/article/110194/ https://doaj.org/toc/2534-9708 doi:10.3897/mbmg.7.110194 2534-9708 https://doaj.org/article/1441a612a62942cd8f7ab29adbb27de4 |
op_doi |
https://doi.org/10.3897/mbmg.7.110194 |
container_title |
Metabarcoding and Metagenomics |
container_volume |
7 |
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1787429592500273152 |