Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiment...

Full description

Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Rômulo Pessoa-e-Silva, Priscilla Stela Santana de Oliveira, Sayonara Maria Calado Gonçalves, Klarissa Miranda Guarines, Lidiane Vasconcelos do Nascimento Carvalho, Maria Andreza Bezerra Correia, Michelle Melgarejo da Rosa, Moacyr Jesus Barreto de Melo Rêgo, Maira Galdino da Rocha Pitta, Michelly Cristiny Pereira
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2021
Subjects:
COVID-19
SARS-CoV-2
Rapid extraction protocol
RT-qPCR
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1590/0037-8682-0270-2021
https://doaj.org/article/14177937e3f348098b611695464c6043
Description
Summary:Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

Similar Items