ICR suckling mouse model of Zika virus infection for disease modeling and drug validation.
BACKGROUND:Zika virus (ZIKV) infection causes diseases ranging from acute self-limiting febrile illness to life-threatening Guillain-Barré Syndrome and other neurological disorders in adults. Cumulative evidence suggests an association between ZIKV infection and microcephaly in newborn infants. Give...
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ftdoajarticles:oai:doaj.org/article:14068a7aa30e4178970fbb21583627e4 2023-05-15T15:09:40+02:00 ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. Yu-Hsuan Wu Chin-Kai Tseng Chun-Kuang Lin Chih-Ku Wei Jin-Ching Lee Kung-Chia Young 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006848 https://doaj.org/article/14068a7aa30e4178970fbb21583627e4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6218097?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006848 https://doaj.org/article/14068a7aa30e4178970fbb21583627e4 PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006848 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006848 2022-12-31T04:31:06Z BACKGROUND:Zika virus (ZIKV) infection causes diseases ranging from acute self-limiting febrile illness to life-threatening Guillain-Barré Syndrome and other neurological disorders in adults. Cumulative evidence suggests an association between ZIKV infection and microcephaly in newborn infants. Given the host-range restrictions of the virus, a susceptible animal model infected by ZIKV must be developed for evaluation of vaccines and antivirals. In this study, we propose a convenient mouse model for analysis of neurological disorders caused by ZIKV. METHODOLOGY:Six-day-old immunocompetent ICR suckling mice were used in the experiment. Different inoculum virus concentrations, challenge routes, and challenge times were assessed. Viremic dissemination was determined in the liver, spleen, kidney, and brain through Western blot assay, plaque assay, absolute quantification real-time PCR, and histological observation. Azithromycin, a well-characterized anti-ZIKV compound, was used to evaluate the ICR suckling mouse model for antiviral testing. CONCLUSIONS:Signs of illness and neurological disease and high mortality rate were observed in mice injected with ZIKV intracerebrally (102 to 105) and intraperitoneally (103 to 105). Viremic dissemination was observed in the liver, spleen, kidney, and brain. ZIKV transmitted, rapid replicated, and induced monocyte infiltration into the brain approximately 5 to 6 days post inoculum. Azithromycin conferred protection against ZIKV-caused neurological and life-threatening diseases. The developed model of ZIKV infection and disease can be used for screening drugs against ZIKV and discovering the underlying mechanism of ZIKV pathogenesis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Barré ENVELOPE(-68.550,-68.550,-67.500,-67.500) PLOS Neglected Tropical Diseases 12 10 e0006848 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yu-Hsuan Wu Chin-Kai Tseng Chun-Kuang Lin Chih-Ku Wei Jin-Ching Lee Kung-Chia Young ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Zika virus (ZIKV) infection causes diseases ranging from acute self-limiting febrile illness to life-threatening Guillain-Barré Syndrome and other neurological disorders in adults. Cumulative evidence suggests an association between ZIKV infection and microcephaly in newborn infants. Given the host-range restrictions of the virus, a susceptible animal model infected by ZIKV must be developed for evaluation of vaccines and antivirals. In this study, we propose a convenient mouse model for analysis of neurological disorders caused by ZIKV. METHODOLOGY:Six-day-old immunocompetent ICR suckling mice were used in the experiment. Different inoculum virus concentrations, challenge routes, and challenge times were assessed. Viremic dissemination was determined in the liver, spleen, kidney, and brain through Western blot assay, plaque assay, absolute quantification real-time PCR, and histological observation. Azithromycin, a well-characterized anti-ZIKV compound, was used to evaluate the ICR suckling mouse model for antiviral testing. CONCLUSIONS:Signs of illness and neurological disease and high mortality rate were observed in mice injected with ZIKV intracerebrally (102 to 105) and intraperitoneally (103 to 105). Viremic dissemination was observed in the liver, spleen, kidney, and brain. ZIKV transmitted, rapid replicated, and induced monocyte infiltration into the brain approximately 5 to 6 days post inoculum. Azithromycin conferred protection against ZIKV-caused neurological and life-threatening diseases. The developed model of ZIKV infection and disease can be used for screening drugs against ZIKV and discovering the underlying mechanism of ZIKV pathogenesis. |
format |
Article in Journal/Newspaper |
author |
Yu-Hsuan Wu Chin-Kai Tseng Chun-Kuang Lin Chih-Ku Wei Jin-Ching Lee Kung-Chia Young |
author_facet |
Yu-Hsuan Wu Chin-Kai Tseng Chun-Kuang Lin Chih-Ku Wei Jin-Ching Lee Kung-Chia Young |
author_sort |
Yu-Hsuan Wu |
title |
ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
title_short |
ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
title_full |
ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
title_fullStr |
ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
title_full_unstemmed |
ICR suckling mouse model of Zika virus infection for disease modeling and drug validation. |
title_sort |
icr suckling mouse model of zika virus infection for disease modeling and drug validation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006848 https://doaj.org/article/14068a7aa30e4178970fbb21583627e4 |
long_lat |
ENVELOPE(-68.550,-68.550,-67.500,-67.500) |
geographic |
Arctic Barré |
geographic_facet |
Arctic Barré |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006848 (2018) |
op_relation |
http://europepmc.org/articles/PMC6218097?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006848 https://doaj.org/article/14068a7aa30e4178970fbb21583627e4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006848 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
10 |
container_start_page |
e0006848 |
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1766340811574738944 |