Antimicrobial activity of latex silver nanoparticles using Calotropis procera
Objective: To synthesize silver nanoparticles (AgNPs) by green methods using serum latex of Calotropis procera at 80 °C and evaluate them against bacteria, dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex. Methods: The synthesis of AgNPs was performed by mixing...
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ftdoajarticles:oai:doaj.org/article:13c97f383c4f4ea2996c55985374964e 2023-05-15T15:11:44+02:00 Antimicrobial activity of latex silver nanoparticles using Calotropis procera Nadia Hussein Mohamed Mady Ahmed Ismail Wael Moustfa Abdel-Mageed Ahmed Abdelfattah Mohamed Shoreit 2014-11-01T00:00:00Z https://doi.org/10.12980/APJTB.4.201414B216 https://doaj.org/article/13c97f383c4f4ea2996c55985374964e EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300344 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B216 https://doaj.org/article/13c97f383c4f4ea2996c55985374964e Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 11, Pp 876-883 (2014) Green synthesis Calotropis procera Latex Silver nanoparticles Antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.201414B216 2022-12-31T10:33:34Z Objective: To synthesize silver nanoparticles (AgNPs) by green methods using serum latex of Calotropis procera at 80 °C and evaluate them against bacteria, dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex. Methods: The synthesis of AgNPs was performed by mixing 3% latex serum extract with the same volume of silver nitrate (2 mmol/L) solution in round flask and heating in water bath at 80 °C. Characterization of silver particles were determined using UV-vis spectrophotometer, transmission electron microscopy (TEM), X-ray diffraction and Fourier transform infrared spectroscopy. The antimicrobial activity of the green synthesized AgNPs was determined against bacteria, dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion methods. Results: Biosynthesis of latex silver nanoparticles was successfully obtained by green method. The formation of AgNPs has been confirmed by UV-vis, TEM microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. TEM analysis showed that synthesized AgNPs are highly stable spherical shaped particles, well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 nm. AgNPs showed strong antibacterial activity against Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum, Candida albicans and Aspergillus terreus. Conclusions: It can be concluded that serum latex of Calotropis procera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions (Ag+ to Ag0). The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 11 876 883 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Green synthesis Calotropis procera Latex Silver nanoparticles Antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Green synthesis Calotropis procera Latex Silver nanoparticles Antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nadia Hussein Mohamed Mady Ahmed Ismail Wael Moustfa Abdel-Mageed Ahmed Abdelfattah Mohamed Shoreit Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
topic_facet |
Green synthesis Calotropis procera Latex Silver nanoparticles Antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To synthesize silver nanoparticles (AgNPs) by green methods using serum latex of Calotropis procera at 80 °C and evaluate them against bacteria, dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex. Methods: The synthesis of AgNPs was performed by mixing 3% latex serum extract with the same volume of silver nitrate (2 mmol/L) solution in round flask and heating in water bath at 80 °C. Characterization of silver particles were determined using UV-vis spectrophotometer, transmission electron microscopy (TEM), X-ray diffraction and Fourier transform infrared spectroscopy. The antimicrobial activity of the green synthesized AgNPs was determined against bacteria, dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion methods. Results: Biosynthesis of latex silver nanoparticles was successfully obtained by green method. The formation of AgNPs has been confirmed by UV-vis, TEM microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. TEM analysis showed that synthesized AgNPs are highly stable spherical shaped particles, well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 nm. AgNPs showed strong antibacterial activity against Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum, Candida albicans and Aspergillus terreus. Conclusions: It can be concluded that serum latex of Calotropis procera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions (Ag+ to Ag0). The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex. |
format |
Article in Journal/Newspaper |
author |
Nadia Hussein Mohamed Mady Ahmed Ismail Wael Moustfa Abdel-Mageed Ahmed Abdelfattah Mohamed Shoreit |
author_facet |
Nadia Hussein Mohamed Mady Ahmed Ismail Wael Moustfa Abdel-Mageed Ahmed Abdelfattah Mohamed Shoreit |
author_sort |
Nadia Hussein Mohamed |
title |
Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
title_short |
Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
title_full |
Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
title_fullStr |
Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
title_full_unstemmed |
Antimicrobial activity of latex silver nanoparticles using Calotropis procera |
title_sort |
antimicrobial activity of latex silver nanoparticles using calotropis procera |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.201414B216 https://doaj.org/article/13c97f383c4f4ea2996c55985374964e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 11, Pp 876-883 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300344 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B216 https://doaj.org/article/13c97f383c4f4ea2996c55985374964e |
op_doi |
https://doi.org/10.12980/APJTB.4.201414B216 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
11 |
container_start_page |
876 |
op_container_end_page |
883 |
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