Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography

To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G g...

Full description

Bibliographic Details
Published in:Acta Biomedica Scientifica
Main Authors: M. K. Vinokurova, O. E. Dogorova, I. S. Malogulova, E. S. Pavlova
Format: Article in Journal/Newspaper
Language:Russian
Published: Scientific Сentre for Family Health and Human Reproduction Problems 2018
Subjects:
Q
Online Access:https://doi.org/10.29413/ABS.2018-3.2.13
https://doaj.org/article/13919349b6e44caebcb4d12810ee8825
id ftdoajarticles:oai:doaj.org/article:13919349b6e44caebcb4d12810ee8825
record_format openpolar
spelling ftdoajarticles:oai:doaj.org/article:13919349b6e44caebcb4d12810ee8825 2024-09-15T18:32:41+00:00 Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography M. K. Vinokurova O. E. Dogorova I. S. Malogulova E. S. Pavlova 2018-04-01T00:00:00Z https://doi.org/10.29413/ABS.2018-3.2.13 https://doaj.org/article/13919349b6e44caebcb4d12810ee8825 RU rus Scientific Сentre for Family Health and Human Reproduction Problems https://www.actabiomedica.ru/jour/article/view/563 https://doaj.org/toc/2541-9420 https://doaj.org/toc/2587-9596 2541-9420 2587-9596 doi:10.29413/ABS.2018-3.2.13 https://doaj.org/article/13919349b6e44caebcb4d12810ee8825 Acta Biomedica Scientifica, Vol 3, Iss 2, Pp 75-79 (2018) mycobacterium tuberculosis genotype isoniazid drug administration routes high pressure liquid chromatography Science Q article 2018 ftdoajarticles https://doi.org/10.29413/ABS.2018-3.2.13 2024-08-05T17:49:16Z To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G gene alone (86.2 %), in both kat G and inh A genes (10.3 %), or in inh A alone (3,5 %). In this work, we studied variations in serum isoniazid concentration in patients with multidrug-resistant tuberculosis, using different routes of drug administration: regional lymphotropic, intravenous infusion (IV), intramuscular injection (IM), and oral. Blood samples were obtained 1.5, 6, and 9 hours after administration of the drug. Levels and variations of isoniazid serum concentrations were assessed at intervals, using high-performance liquid chromatography (HPLC). For assessing the efficiency of treatment, patients were divided to four groups according to isoniazid administration routes. The study established that serum isoniazid concentrations observed with regional lymphotropic administration, were initially the lowest (4.2 mg/L), compared to IV infusion, IM, and oral administration routes (8,12.5, and 17.1 mg/L, respectively), but showed slower reduction of concentration. It was also noticed, that after 9 hours, the serum concentration of isoniazid was reliably higher in regional lymphotropicgroup, than in the rest of study groups (2.2 vs. 0.8 mg/L). Higher therapeutic effect of intralymphatic drug administration could be explained by the formation within the regional lymphatic network of a localized area around the site of injection containing enhanced concentration of isoniazid and allowing extended drug release into bloodstream, resulting further in prolonged drug biotransformation. Article in Journal/Newspaper Sakha Republic Yakutia Directory of Open Access Journals: DOAJ Articles Acta Biomedica Scientifica 3 2 75 79
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language Russian
topic mycobacterium tuberculosis
genotype
isoniazid
drug administration routes
high pressure liquid chromatography
Science
Q
spellingShingle mycobacterium tuberculosis
genotype
isoniazid
drug administration routes
high pressure liquid chromatography
Science
Q
M. K. Vinokurova
O. E. Dogorova
I. S. Malogulova
E. S. Pavlova
Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
topic_facet mycobacterium tuberculosis
genotype
isoniazid
drug administration routes
high pressure liquid chromatography
Science
Q
description To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G gene alone (86.2 %), in both kat G and inh A genes (10.3 %), or in inh A alone (3,5 %). In this work, we studied variations in serum isoniazid concentration in patients with multidrug-resistant tuberculosis, using different routes of drug administration: regional lymphotropic, intravenous infusion (IV), intramuscular injection (IM), and oral. Blood samples were obtained 1.5, 6, and 9 hours after administration of the drug. Levels and variations of isoniazid serum concentrations were assessed at intervals, using high-performance liquid chromatography (HPLC). For assessing the efficiency of treatment, patients were divided to four groups according to isoniazid administration routes. The study established that serum isoniazid concentrations observed with regional lymphotropic administration, were initially the lowest (4.2 mg/L), compared to IV infusion, IM, and oral administration routes (8,12.5, and 17.1 mg/L, respectively), but showed slower reduction of concentration. It was also noticed, that after 9 hours, the serum concentration of isoniazid was reliably higher in regional lymphotropicgroup, than in the rest of study groups (2.2 vs. 0.8 mg/L). Higher therapeutic effect of intralymphatic drug administration could be explained by the formation within the regional lymphatic network of a localized area around the site of injection containing enhanced concentration of isoniazid and allowing extended drug release into bloodstream, resulting further in prolonged drug biotransformation.
format Article in Journal/Newspaper
author M. K. Vinokurova
O. E. Dogorova
I. S. Malogulova
E. S. Pavlova
author_facet M. K. Vinokurova
O. E. Dogorova
I. S. Malogulova
E. S. Pavlova
author_sort M. K. Vinokurova
title Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_short Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_full Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_fullStr Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_full_unstemmed Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_sort detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
publisher Scientific Сentre for Family Health and Human Reproduction Problems
publishDate 2018
url https://doi.org/10.29413/ABS.2018-3.2.13
https://doaj.org/article/13919349b6e44caebcb4d12810ee8825
genre Sakha Republic
Yakutia
genre_facet Sakha Republic
Yakutia
op_source Acta Biomedica Scientifica, Vol 3, Iss 2, Pp 75-79 (2018)
op_relation https://www.actabiomedica.ru/jour/article/view/563
https://doaj.org/toc/2541-9420
https://doaj.org/toc/2587-9596
2541-9420
2587-9596
doi:10.29413/ABS.2018-3.2.13
https://doaj.org/article/13919349b6e44caebcb4d12810ee8825
op_doi https://doi.org/10.29413/ABS.2018-3.2.13
container_title Acta Biomedica Scientifica
container_volume 3
container_issue 2
container_start_page 75
op_container_end_page 79
_version_ 1810474412320751616