Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia
Abstract Background Pregnant women frequently show low-density Plasmodium infections that require more sensitive methods for accurate diagnosis and early treatment of malaria. This is particularly relevant in low-malaria transmission areas, where intermittent preventive treatment is not recommended....
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ftdoajarticles:oai:doaj.org/article:13609820da4f4d15afba0892938635ab 2023-05-15T15:17:34+02:00 Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia Ana María Vásquez Lina Zuluaga Alberto Tobón Maritza Posada Gabriel Vélez Iveth J. González Ana Campillo Xavier Ding 2018-07-01T00:00:00Z https://doi.org/10.1186/s12936-018-2403-5 https://doaj.org/article/13609820da4f4d15afba0892938635ab EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2403-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2403-5 1475-2875 https://doaj.org/article/13609820da4f4d15afba0892938635ab Malaria Journal, Vol 17, Iss 1, Pp 1-11 (2018) Malaria in pregnancy Diagnostics Rapid diagnostic test Light microscopy Loop mediated isothermal amplification Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2403-5 2022-12-31T11:57:15Z Abstract Background Pregnant women frequently show low-density Plasmodium infections that require more sensitive methods for accurate diagnosis and early treatment of malaria. This is particularly relevant in low-malaria transmission areas, where intermittent preventive treatment is not recommended. Molecular methods, such as polymerase chain reaction (PCR) are highly sensitive, but require sophisticated equipment and advanced training. Instead, loop mediated isothermal amplification (LAMP) provides an opportunity for molecular detection of malaria infections in remote endemic areas, outside a reference laboratory. The aim of the study is to evaluate the performance of LAMP for the screening of malaria in pregnant women in Colombia. Methods This is a nested prospective study that uses data and samples from a larger cross-sectional project conducted from May 2016 to January 2017 in three Colombian endemic areas (El Bagre, Quibdó, and Tumaco). A total of 531 peripheral and placental samples from pregnant women self-presenting at local hospitals for antenatal care visits, at delivery or seeking medical care for suspected malaria were collected. Samples were analysed for Plasmodium parasites by light microscopy (LM), rapid diagnostic test (RDT) and LAMP. Diagnostic accuracy endpoints (sensitivity, specificity, predictive values, and kappa scores) of LM, RDT and LAMP were compared with nested PCR (nPCR) as the reference standard. Results In peripheral samples, LAMP showed an improved sensitivity (100.0%) when compared with LM 79.5% and RDT 76.9% (p < 0.01), particularly in afebrile women, for which LAMP sensitivity was two-times higher than LM and RDT. Overall agreement among LAMP and nPCR was high (kappa value = 1.0). Specificity was similar in all tests (100%). In placental blood, LAMP evidenced a four-fold improvement in sensitivity (88.9%) when compared with LM and RDT (22.2%), being the only method, together with nPCR, able to detect placental infections in peripheral blood. Conclusions LAMP is a simple, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Malaria in pregnancy Diagnostics Rapid diagnostic test Light microscopy Loop mediated isothermal amplification Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria in pregnancy Diagnostics Rapid diagnostic test Light microscopy Loop mediated isothermal amplification Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ana María Vásquez Lina Zuluaga Alberto Tobón Maritza Posada Gabriel Vélez Iveth J. González Ana Campillo Xavier Ding Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
topic_facet |
Malaria in pregnancy Diagnostics Rapid diagnostic test Light microscopy Loop mediated isothermal amplification Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Pregnant women frequently show low-density Plasmodium infections that require more sensitive methods for accurate diagnosis and early treatment of malaria. This is particularly relevant in low-malaria transmission areas, where intermittent preventive treatment is not recommended. Molecular methods, such as polymerase chain reaction (PCR) are highly sensitive, but require sophisticated equipment and advanced training. Instead, loop mediated isothermal amplification (LAMP) provides an opportunity for molecular detection of malaria infections in remote endemic areas, outside a reference laboratory. The aim of the study is to evaluate the performance of LAMP for the screening of malaria in pregnant women in Colombia. Methods This is a nested prospective study that uses data and samples from a larger cross-sectional project conducted from May 2016 to January 2017 in three Colombian endemic areas (El Bagre, Quibdó, and Tumaco). A total of 531 peripheral and placental samples from pregnant women self-presenting at local hospitals for antenatal care visits, at delivery or seeking medical care for suspected malaria were collected. Samples were analysed for Plasmodium parasites by light microscopy (LM), rapid diagnostic test (RDT) and LAMP. Diagnostic accuracy endpoints (sensitivity, specificity, predictive values, and kappa scores) of LM, RDT and LAMP were compared with nested PCR (nPCR) as the reference standard. Results In peripheral samples, LAMP showed an improved sensitivity (100.0%) when compared with LM 79.5% and RDT 76.9% (p < 0.01), particularly in afebrile women, for which LAMP sensitivity was two-times higher than LM and RDT. Overall agreement among LAMP and nPCR was high (kappa value = 1.0). Specificity was similar in all tests (100%). In placental blood, LAMP evidenced a four-fold improvement in sensitivity (88.9%) when compared with LM and RDT (22.2%), being the only method, together with nPCR, able to detect placental infections in peripheral blood. Conclusions LAMP is a simple, ... |
format |
Article in Journal/Newspaper |
author |
Ana María Vásquez Lina Zuluaga Alberto Tobón Maritza Posada Gabriel Vélez Iveth J. González Ana Campillo Xavier Ding |
author_facet |
Ana María Vásquez Lina Zuluaga Alberto Tobón Maritza Posada Gabriel Vélez Iveth J. González Ana Campillo Xavier Ding |
author_sort |
Ana María Vásquez |
title |
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
title_short |
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
title_full |
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
title_fullStr |
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
title_full_unstemmed |
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia |
title_sort |
diagnostic accuracy of loop-mediated isothermal amplification (lamp) for screening malaria in peripheral and placental blood samples from pregnant women in colombia |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2403-5 https://doaj.org/article/13609820da4f4d15afba0892938635ab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-11 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2403-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2403-5 1475-2875 https://doaj.org/article/13609820da4f4d15afba0892938635ab |
op_doi |
https://doi.org/10.1186/s12936-018-2403-5 |
container_title |
Malaria Journal |
container_volume |
17 |
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1 |
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1766347815220412416 |