Anti-Candida and anti-Cryptococcus evaluation of 15 non-alkaloidal compounds from Pterogyne nitens

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Caroline Sprengel Lima, Carlos Roberto Polaquini, Mariana Bastos dos Santos, Fernanda Patrícia Gullo, Fernanda Sangalli Leite, Liliane Scorzoni, Vanderlan da Silva Bolzani, Maria José Soares Mendes-Giannini, Ana Marisa Fusco-Almeida, Andréia Alves Rezende, Luis Octavio Regasini
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2016
• Subjects: Candida; Cryptococcus; Antifungal; Pterogyne nitens; Flavonoid; Opportunistic fungi; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2016.08.003; https://doaj.org/article/1359685ecffb451b886251f68476809c

Objective: To evaluate anti-Candida and anti-Cryptococcus activities of 15 non-alkaloidal compounds from Pterogyne nitens Tulasne (Leguminosae), a South American medicinal plant. Methods: Compounds were submitted to antifungal assays, using microdilution method described by Clinical and Laboratory Standards Institute document, with minor modifications. Five species of Candida and two species of Cryptococcus, including clinical isolates were screened. Antifungal activity was expressed by minimum inhibitory concentration (MIC). Amphotericin B and fluconazole were used as standard antifungal drugs. Results: Among tested compounds, six substances presented fungal growth inhibition (MIC < 31.2 μg/mL) [three flavone derivatives (1–3), a glycosylated flavonol derivative (5) and two phenolic acids (10 and 12)]. Sorbifolin (1), exhibited potent antifungal activity, demonstrating MIC value of 3.90 μg/mL against Candida glabrata ATCC 90030, Cryptococcus gattii 118 and fluconazole-resistant clinical isolate of Cryptococcus neoformans var. grubii. Pedalin (2) and nitensoside B (3), two glycosylated flavone derivatives, were active against Cryptococcus neoformans ATCC 90012 (MIC = 7.80 μg/mL). Conclusions: Flavone derivatives from Pterogyne nitens can serve as prototypes for the design and development of innovative anti-Candida and anti-Cryptococcus hits.