Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair
Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regen...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:135813bc2517467a88f37c6d00130c01 2023-05-15T15:18:28+02:00 Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair Roghayeh Mozafari Sergiy Kyrylenko Mateus Vidigal Castro Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues Oliveira 2018-04-01T00:00:00Z https://doi.org/10.1186/s40409-018-0147-x https://doaj.org/article/135813bc2517467a88f37c6d00130c01 EN eng SciELO http://link.springer.com/article/10.1186/s40409-018-0147-x https://doaj.org/toc/1678-9199 doi:10.1186/s40409-018-0147-x 1678-9199 https://doaj.org/article/135813bc2517467a88f37c6d00130c01 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-16 (2018) Neurorrhaphy Sciatic nerve Human embryonic stem cells Fibrin sealant FGF2 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1186/s40409-018-0147-x 2023-01-08T01:29:49Z Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability. Therefore, the main objective of this study was to find conditions under which functional recovery is improved after sciatic nerve neurorrhaphy. We assumed that hESC, either alone or in combination with heterologous fibrin sealant scaffold, could be used to support regeneration in a mouse model of sciatic nerve injury and repair via autografting with end-to-end neurorrhaphy. Methods Five millimeters of the sciatic nerve of C57BL/6 J mice were transected off and rotated 180 degrees to simulate an injury, and then stumps were sutured. Next, we applied heterologous fibrin sealant and/or human embryonic stem cells genetically altered to overexpress fibroblast growth factor 2 (FGF2) at the site of the injury. The study was designed to include six experimental groups comprising neurorrhaphy (N), neurorrhaphy + heterologous fibrin sealant (N + F), neurorrhaphy + heterologous fibrin sealant + doxycycline (N + F + D), neurorrhaphy + heterologous fibrin sealant + wild-type hESC (N + F + W), neurorrhaphy + heterologous fibrin sealant + hESC off (N + F + T), and neurorrhaphy + heterologous fibrin sealant + hESC on via doxycycline (N + F + D + T). We evaluated the recovery rate using Catwalk and von Frey functional recovery tests, as well as immunohistochemistry analysis. Results The experiments indicated that sensory function improved when transgenic hESCs were ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Catwalk ENVELOPE(-60.923,-60.923,-64.506,-64.506) Journal of Venomous Animals and Toxins including Tropical Diseases 24 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Neurorrhaphy Sciatic nerve Human embryonic stem cells Fibrin sealant FGF2 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Neurorrhaphy Sciatic nerve Human embryonic stem cells Fibrin sealant FGF2 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Roghayeh Mozafari Sergiy Kyrylenko Mateus Vidigal Castro Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues Oliveira Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
topic_facet |
Neurorrhaphy Sciatic nerve Human embryonic stem cells Fibrin sealant FGF2 Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability. Therefore, the main objective of this study was to find conditions under which functional recovery is improved after sciatic nerve neurorrhaphy. We assumed that hESC, either alone or in combination with heterologous fibrin sealant scaffold, could be used to support regeneration in a mouse model of sciatic nerve injury and repair via autografting with end-to-end neurorrhaphy. Methods Five millimeters of the sciatic nerve of C57BL/6 J mice were transected off and rotated 180 degrees to simulate an injury, and then stumps were sutured. Next, we applied heterologous fibrin sealant and/or human embryonic stem cells genetically altered to overexpress fibroblast growth factor 2 (FGF2) at the site of the injury. The study was designed to include six experimental groups comprising neurorrhaphy (N), neurorrhaphy + heterologous fibrin sealant (N + F), neurorrhaphy + heterologous fibrin sealant + doxycycline (N + F + D), neurorrhaphy + heterologous fibrin sealant + wild-type hESC (N + F + W), neurorrhaphy + heterologous fibrin sealant + hESC off (N + F + T), and neurorrhaphy + heterologous fibrin sealant + hESC on via doxycycline (N + F + D + T). We evaluated the recovery rate using Catwalk and von Frey functional recovery tests, as well as immunohistochemistry analysis. Results The experiments indicated that sensory function improved when transgenic hESCs were ... |
format |
Article in Journal/Newspaper |
author |
Roghayeh Mozafari Sergiy Kyrylenko Mateus Vidigal Castro Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues Oliveira |
author_facet |
Roghayeh Mozafari Sergiy Kyrylenko Mateus Vidigal Castro Rui Seabra Ferreira Benedito Barraviera Alexandre Leite Rodrigues Oliveira |
author_sort |
Roghayeh Mozafari |
title |
Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
title_short |
Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
title_full |
Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
title_fullStr |
Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
title_full_unstemmed |
Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
title_sort |
combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair |
publisher |
SciELO |
publishDate |
2018 |
url |
https://doi.org/10.1186/s40409-018-0147-x https://doaj.org/article/135813bc2517467a88f37c6d00130c01 |
long_lat |
ENVELOPE(-60.923,-60.923,-64.506,-64.506) |
geographic |
Arctic Catwalk |
geographic_facet |
Arctic Catwalk |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-16 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s40409-018-0147-x https://doaj.org/toc/1678-9199 doi:10.1186/s40409-018-0147-x 1678-9199 https://doaj.org/article/135813bc2517467a88f37c6d00130c01 |
op_doi |
https://doi.org/10.1186/s40409-018-0147-x |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
24 |
container_issue |
1 |
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1766348667315290112 |