Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus
We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10ºC, though the...
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ftdoajarticles:oai:doaj.org/article:132d3ebeae094011908f2a40381a6999 2023-05-15T13:03:38+02:00 Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus Hiroshi Kagoshima Satoshi Imura Atsushi C. Suzuki 2013-03-01T00:00:00Z https://doi.org/10.4081/jlimnol.2013.s1.e3 https://doaj.org/article/132d3ebeae094011908f2a40381a6999 EN eng PAGEPress Publications http://www.jlimnol.it/index.php/jlimnol/article/view/744 https://doaj.org/toc/1129-5767 https://doaj.org/toc/1723-8633 1129-5767 1723-8633 doi:10.4081/jlimnol.2013.s1.e3 https://doaj.org/article/132d3ebeae094011908f2a40381a6999 Journal of Limnology, Vol 72, Iss 1s, Pp e3-e3 (2013) Tardigrada antarctica cultivation exuviae JARE (Japanese Antarctic Research Expedition) 18S rRNA Geography. Anthropology. Recreation G Physical geography GB3-5030 Environmental sciences GE1-350 article 2013 ftdoajarticles https://doi.org/10.4081/jlimnol.2013.s1.e3 2022-12-31T10:33:52Z We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10ºC, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that of Acutuncus antarcticus from King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of A. antarcticus : dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake. Article in Journal/Newspaper Acutuncus antarcticus Antarc* Antarctic Antarctica antarcticus King George Island South Shetland Islands Tardigrade Directory of Open Access Journals: DOAJ Articles Antarctic King George Island Syowa Station South Shetland Islands Langhovde ENVELOPE(39.733,39.733,-69.217,-69.217) Skarvsnes ENVELOPE(39.667,39.667,-69.467,-69.467) Hotoke Ike ENVELOPE(39.567,39.567,-69.477,-69.477) Journal of Limnology 72 1s |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tardigrada antarctica cultivation exuviae JARE (Japanese Antarctic Research Expedition) 18S rRNA Geography. Anthropology. Recreation G Physical geography GB3-5030 Environmental sciences GE1-350 |
spellingShingle |
Tardigrada antarctica cultivation exuviae JARE (Japanese Antarctic Research Expedition) 18S rRNA Geography. Anthropology. Recreation G Physical geography GB3-5030 Environmental sciences GE1-350 Hiroshi Kagoshima Satoshi Imura Atsushi C. Suzuki Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
topic_facet |
Tardigrada antarctica cultivation exuviae JARE (Japanese Antarctic Research Expedition) 18S rRNA Geography. Anthropology. Recreation G Physical geography GB3-5030 Environmental sciences GE1-350 |
description |
We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10ºC, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that of Acutuncus antarcticus from King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of A. antarcticus : dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake. |
format |
Article in Journal/Newspaper |
author |
Hiroshi Kagoshima Satoshi Imura Atsushi C. Suzuki |
author_facet |
Hiroshi Kagoshima Satoshi Imura Atsushi C. Suzuki |
author_sort |
Hiroshi Kagoshima |
title |
Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
title_short |
Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
title_full |
Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
title_fullStr |
Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
title_full_unstemmed |
Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus |
title_sort |
molecular and morphological analysis of an antarctic tardigrade, acutuncus antarcticus |
publisher |
PAGEPress Publications |
publishDate |
2013 |
url |
https://doi.org/10.4081/jlimnol.2013.s1.e3 https://doaj.org/article/132d3ebeae094011908f2a40381a6999 |
long_lat |
ENVELOPE(39.733,39.733,-69.217,-69.217) ENVELOPE(39.667,39.667,-69.467,-69.467) ENVELOPE(39.567,39.567,-69.477,-69.477) |
geographic |
Antarctic King George Island Syowa Station South Shetland Islands Langhovde Skarvsnes Hotoke Ike |
geographic_facet |
Antarctic King George Island Syowa Station South Shetland Islands Langhovde Skarvsnes Hotoke Ike |
genre |
Acutuncus antarcticus Antarc* Antarctic Antarctica antarcticus King George Island South Shetland Islands Tardigrade |
genre_facet |
Acutuncus antarcticus Antarc* Antarctic Antarctica antarcticus King George Island South Shetland Islands Tardigrade |
op_source |
Journal of Limnology, Vol 72, Iss 1s, Pp e3-e3 (2013) |
op_relation |
http://www.jlimnol.it/index.php/jlimnol/article/view/744 https://doaj.org/toc/1129-5767 https://doaj.org/toc/1723-8633 1129-5767 1723-8633 doi:10.4081/jlimnol.2013.s1.e3 https://doaj.org/article/132d3ebeae094011908f2a40381a6999 |
op_doi |
https://doi.org/10.4081/jlimnol.2013.s1.e3 |
container_title |
Journal of Limnology |
container_volume |
72 |
container_issue |
1s |
_version_ |
1766341322099130368 |