Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum
Calf serum and fetal bovine serum present great variability as to its growth promoting efficiency (GPE). As supplement of culture media to cultivate cells of animal origin they stimulate the "in vitro" multiplication and maintain cell viability. When fourteen lots of calf sera of variable...
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Universidade de São Paulo (USP)
1984
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ftdoajarticles:oai:doaj.org/article:1327533fe4ec4063be79fd67c38e38d8 2024-09-09T19:27:24+00:00 Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum Edda de Rizzo Celidéia Aparecida Coppi Vaz Inácio França Mendes Anatércia Ferreira Bonfim Yano 1984-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651984000200005 https://doaj.org/article/1327533fe4ec4063be79fd67c38e38d8 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651984000200005&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651984000200005 https://doaj.org/article/1327533fe4ec4063be79fd67c38e38d8 Revista do Instituto de Medicina Tropical de São Paulo, Vol 26, Iss 2, Pp 97-104 (1984) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1984 ftdoajarticles https://doi.org/10.1590/S0036-46651984000200005 2024-08-05T17:49:30Z Calf serum and fetal bovine serum present great variability as to its growth promoting efficiency (GPE). As supplement of culture media to cultivate cells of animal origin they stimulate the "in vitro" multiplication and maintain cell viability. When fourteen lots of calf sera of variable GPE had the total protein contents as well as the percentages of serum fractions determined, no significant differences that could possibly explain the variability of the GPE were observed. Evaluation of the antiproteolytic activity of nineteen lots of calf serum and eighteen serum lots of younger calves showed that the former exhibited lower antiproteolytic titers (1:40 to 1:80) than the latter (1:80 to 1:160). Twelve lots of fetal bovine serum studied in parallel, showed the highest concentration of antiproteolytic factors, with titers equal to 1:320. Sera of bovine origin, but not fetal sera, are usually heat-inactivated, what was demonstrated to be responsible for the decrease of the antiproteolytic activity of 75% of the lots tested. This could explain the inability of certain heat-inactivated sera in promoting multiplication of some cells "in vitro", as verified with primary monkey kidney cells. The results obtained in this study indicated the convenience of submiting each lot of serum to be introduced in cell culture to previous determination of its characteristics, such as growth promoting efficiency, antiproteolytic activity and also toxicity, absence of extraneous agents, etc., in order to minimize the possibility of using serum lots of questionable quality, thus preventing not only the loss of cell lines, but also undesirable and sometimes expensive delays. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 26 2 97 104 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Edda de Rizzo Celidéia Aparecida Coppi Vaz Inácio França Mendes Anatércia Ferreira Bonfim Yano Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Calf serum and fetal bovine serum present great variability as to its growth promoting efficiency (GPE). As supplement of culture media to cultivate cells of animal origin they stimulate the "in vitro" multiplication and maintain cell viability. When fourteen lots of calf sera of variable GPE had the total protein contents as well as the percentages of serum fractions determined, no significant differences that could possibly explain the variability of the GPE were observed. Evaluation of the antiproteolytic activity of nineteen lots of calf serum and eighteen serum lots of younger calves showed that the former exhibited lower antiproteolytic titers (1:40 to 1:80) than the latter (1:80 to 1:160). Twelve lots of fetal bovine serum studied in parallel, showed the highest concentration of antiproteolytic factors, with titers equal to 1:320. Sera of bovine origin, but not fetal sera, are usually heat-inactivated, what was demonstrated to be responsible for the decrease of the antiproteolytic activity of 75% of the lots tested. This could explain the inability of certain heat-inactivated sera in promoting multiplication of some cells "in vitro", as verified with primary monkey kidney cells. The results obtained in this study indicated the convenience of submiting each lot of serum to be introduced in cell culture to previous determination of its characteristics, such as growth promoting efficiency, antiproteolytic activity and also toxicity, absence of extraneous agents, etc., in order to minimize the possibility of using serum lots of questionable quality, thus preventing not only the loss of cell lines, but also undesirable and sometimes expensive delays. |
format |
Article in Journal/Newspaper |
author |
Edda de Rizzo Celidéia Aparecida Coppi Vaz Inácio França Mendes Anatércia Ferreira Bonfim Yano |
author_facet |
Edda de Rizzo Celidéia Aparecida Coppi Vaz Inácio França Mendes Anatércia Ferreira Bonfim Yano |
author_sort |
Edda de Rizzo |
title |
Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
title_short |
Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
title_full |
Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
title_fullStr |
Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
title_full_unstemmed |
Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
title_sort |
study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" ii: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1984 |
url |
https://doi.org/10.1590/S0036-46651984000200005 https://doaj.org/article/1327533fe4ec4063be79fd67c38e38d8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 26, Iss 2, Pp 97-104 (1984) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651984000200005&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651984000200005 https://doaj.org/article/1327533fe4ec4063be79fd67c38e38d8 |
op_doi |
https://doi.org/10.1590/S0036-46651984000200005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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26 |
container_issue |
2 |
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97 |
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104 |
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