Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort.
Background Reports have shown correlations between the immune response to vector saliva and Leishmaniasis outcome. We followed dogs in an endemic area for two years characterizing resistance or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania infantum diagnosis and clini...
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ftdoajarticles:oai:doaj.org/article:13118378abb74f59830dfc3e7889e4a9 2023-05-15T15:15:31+02:00 Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. Manuela da Silva Solcà Maiara Reis Arruda Bruna Martins Macedo Leite Tiago Feitosa Mota Miriam Flores Rebouças Matheus Silva de Jesus Leila Denise Alves Ferreira Amorim Valéria Matos Borges Jesus Valenzuela Shaden Kamhawi Patrícia Sampaio Tavares Veras Deborah Bittencourt Mothé Fraga Claudia Ida Brodskyn 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009137 https://doaj.org/article/13118378abb74f59830dfc3e7889e4a9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009137 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009137 https://doaj.org/article/13118378abb74f59830dfc3e7889e4a9 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009137 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009137 2022-12-31T11:55:03Z Background Reports have shown correlations between the immune response to vector saliva and Leishmaniasis outcome. We followed dogs in an endemic area for two years characterizing resistance or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania infantum diagnosis and clinical development criteria. Then, we aimed to identify a biosignature based on parasite load, serum biological mediators' interactions, and vector exposure intensity associated with CVL resistance and susceptibility. Methodology/principal findings A prospective two-year study was conducted in an area endemic for CVL. Dogs were evaluated at 6-month intervals to determine infection, clinical manifestations, immune profile, and sandfly exposure. CVL resistance or susceptibility was determined upon the conclusion of the study. After two years, 78% of the dogs were infected with L. infantum (53% susceptible and 47% resistant to CVL). Susceptible dogs presented higher splenic parasite load as well as persistence of the parasite during the follow-up, compared to resistant ones. Susceptible dogs also displayed a higher number of correlations among the investigated biological mediators, before and after infection diagnosis. At baseline, anti-saliva antibodies, indicative of exposure to the vector, were detected in 62% of the dogs, reaching 100% in one year. Higher sandfly exposure increased the risk of susceptibility to CVL by 1.6 times (CI: 1.11-2.41). We identified a discriminatory biosignature between the resistant and susceptible dogs assessing splenic parasite load, interaction of biological mediators, PGE2 serum levels and intensity of exposure to sandfly. All these parameters were elevated in susceptible dogs compared to resistant animals. Conclusions/significance The biosignature identified in our study reinforces the idea that CVL is a complex multifactorial disease that is affected by a set of factors which are correlated and, for a better understanding of CVL, should not be evaluated in an isolated way. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0009137 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Manuela da Silva Solcà Maiara Reis Arruda Bruna Martins Macedo Leite Tiago Feitosa Mota Miriam Flores Rebouças Matheus Silva de Jesus Leila Denise Alves Ferreira Amorim Valéria Matos Borges Jesus Valenzuela Shaden Kamhawi Patrícia Sampaio Tavares Veras Deborah Bittencourt Mothé Fraga Claudia Ida Brodskyn Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Reports have shown correlations between the immune response to vector saliva and Leishmaniasis outcome. We followed dogs in an endemic area for two years characterizing resistance or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania infantum diagnosis and clinical development criteria. Then, we aimed to identify a biosignature based on parasite load, serum biological mediators' interactions, and vector exposure intensity associated with CVL resistance and susceptibility. Methodology/principal findings A prospective two-year study was conducted in an area endemic for CVL. Dogs were evaluated at 6-month intervals to determine infection, clinical manifestations, immune profile, and sandfly exposure. CVL resistance or susceptibility was determined upon the conclusion of the study. After two years, 78% of the dogs were infected with L. infantum (53% susceptible and 47% resistant to CVL). Susceptible dogs presented higher splenic parasite load as well as persistence of the parasite during the follow-up, compared to resistant ones. Susceptible dogs also displayed a higher number of correlations among the investigated biological mediators, before and after infection diagnosis. At baseline, anti-saliva antibodies, indicative of exposure to the vector, were detected in 62% of the dogs, reaching 100% in one year. Higher sandfly exposure increased the risk of susceptibility to CVL by 1.6 times (CI: 1.11-2.41). We identified a discriminatory biosignature between the resistant and susceptible dogs assessing splenic parasite load, interaction of biological mediators, PGE2 serum levels and intensity of exposure to sandfly. All these parameters were elevated in susceptible dogs compared to resistant animals. Conclusions/significance The biosignature identified in our study reinforces the idea that CVL is a complex multifactorial disease that is affected by a set of factors which are correlated and, for a better understanding of CVL, should not be evaluated in an isolated way. |
format |
Article in Journal/Newspaper |
author |
Manuela da Silva Solcà Maiara Reis Arruda Bruna Martins Macedo Leite Tiago Feitosa Mota Miriam Flores Rebouças Matheus Silva de Jesus Leila Denise Alves Ferreira Amorim Valéria Matos Borges Jesus Valenzuela Shaden Kamhawi Patrícia Sampaio Tavares Veras Deborah Bittencourt Mothé Fraga Claudia Ida Brodskyn |
author_facet |
Manuela da Silva Solcà Maiara Reis Arruda Bruna Martins Macedo Leite Tiago Feitosa Mota Miriam Flores Rebouças Matheus Silva de Jesus Leila Denise Alves Ferreira Amorim Valéria Matos Borges Jesus Valenzuela Shaden Kamhawi Patrícia Sampaio Tavares Veras Deborah Bittencourt Mothé Fraga Claudia Ida Brodskyn |
author_sort |
Manuela da Silva Solcà |
title |
Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
title_short |
Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
title_full |
Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
title_fullStr |
Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
title_full_unstemmed |
Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
title_sort |
immune response dynamics and lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009137 https://doaj.org/article/13118378abb74f59830dfc3e7889e4a9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009137 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009137 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009137 https://doaj.org/article/13118378abb74f59830dfc3e7889e4a9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009137 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
container_start_page |
e0009137 |
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1766345885364518912 |