Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children
Abstract Background The FcγRs genotypes have been reported to play a key role in the defence against malaria parasites through both cellular and humoral immunity. This study aimed to investigate the possible correlation between FcγR (IIa, IIIa, and IIIb) genes polymorphism and the clinical outcome f...
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ftdoajarticles:oai:doaj.org/article:12fa7e86a08c4dfe8c68089edae082c5 2023-05-15T15:17:11+02:00 Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children Amre Nasr Ahmad Aljada Osama Hamid Hatim A. Elsheikh Emad Masuadi Ahmad Al-Bawab Themer H. Alenazi Amir Abushouk Ayman M. Salah 2021-09-01T00:00:00Z https://doi.org/10.1186/s12936-021-03901-0 https://doaj.org/article/12fa7e86a08c4dfe8c68089edae082c5 EN eng BMC https://doi.org/10.1186/s12936-021-03901-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03901-0 1475-2875 https://doaj.org/article/12fa7e86a08c4dfe8c68089edae082c5 Malaria Journal, Vol 20, Iss 1, Pp 1-10 (2021) Plasmodium Falciparum Malaria IgG subclass AMA-1 Saudi Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03901-0 2022-12-31T12:50:15Z Abstract Background The FcγRs genotypes have been reported to play a key role in the defence against malaria parasites through both cellular and humoral immunity. This study aimed to investigate the possible correlation between FcγR (IIa, IIIa, and IIIb) genes polymorphism and the clinical outcome for anti‐malarial antibody response of Plasmodium falciparum infection among Saudi children. Methods A total of 600 volunteers were enrolled in this study, including 200 malaria-free control (MFC) subjects, 218 patients with uncomplicated malaria (UM) and 182 patients with severe malaria (SM). The FcγR genotypes were analysed using PCR amplification methods, and measurements of immunoglobulin were determined using enzyme-linked immunosorbent assay (ELISA) technique. Results The data revealed that the FcγRIIa-R/R131 showed a statistically significant association with SM patients when compared to UM patients. Furthermore, higher levels of IgG1, IgG2, and IgG4 were associated with the FcγRIIa-H/H131 genotype among UM patients. Although the FcγRIIa-F/V176 genotype was not associated with UM, it showed a significant association with severe malaria. Interestingly, the FcγRIIIa-V/V176 genotype offered protection against SM. Moreover, SM patients carrying the FcγRIIIa-F/F genotype showed higher levels of AMA-1-specific IgG2 and IgG4 antibodies. The FcγRIIIb-NA1/NA1 and FcγRIIIb-NA2/NA2 genotypes did not show significant differences between the UM and the MFC groups. However, the genotype FcγRIIIb-NA2/NA2 was statistically significantly associated with SM patients. Conclusions The data presented in this study suggest that the influence of the FcγRIIa-R/R131, FcγRIIIa-F/F176 and FcγRIIIb-NA2/NA2 genotypes are statistically significantly associated with SM patients. However, the FcγRIIa-H/H13 and FcγRIIIa-V/V176 genotypes have demonstrated a protective effect against SM when compared to UM patients. The impact of the FcyR (IIa, IIIa and IIIb) gene variants and anti-malaria IgG subclasses play an important role in susceptibility ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Plasmodium Falciparum Malaria IgG subclass AMA-1 Saudi Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium Falciparum Malaria IgG subclass AMA-1 Saudi Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Amre Nasr Ahmad Aljada Osama Hamid Hatim A. Elsheikh Emad Masuadi Ahmad Al-Bawab Themer H. Alenazi Amir Abushouk Ayman M. Salah Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
topic_facet |
Plasmodium Falciparum Malaria IgG subclass AMA-1 Saudi Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The FcγRs genotypes have been reported to play a key role in the defence against malaria parasites through both cellular and humoral immunity. This study aimed to investigate the possible correlation between FcγR (IIa, IIIa, and IIIb) genes polymorphism and the clinical outcome for anti‐malarial antibody response of Plasmodium falciparum infection among Saudi children. Methods A total of 600 volunteers were enrolled in this study, including 200 malaria-free control (MFC) subjects, 218 patients with uncomplicated malaria (UM) and 182 patients with severe malaria (SM). The FcγR genotypes were analysed using PCR amplification methods, and measurements of immunoglobulin were determined using enzyme-linked immunosorbent assay (ELISA) technique. Results The data revealed that the FcγRIIa-R/R131 showed a statistically significant association with SM patients when compared to UM patients. Furthermore, higher levels of IgG1, IgG2, and IgG4 were associated with the FcγRIIa-H/H131 genotype among UM patients. Although the FcγRIIa-F/V176 genotype was not associated with UM, it showed a significant association with severe malaria. Interestingly, the FcγRIIIa-V/V176 genotype offered protection against SM. Moreover, SM patients carrying the FcγRIIIa-F/F genotype showed higher levels of AMA-1-specific IgG2 and IgG4 antibodies. The FcγRIIIb-NA1/NA1 and FcγRIIIb-NA2/NA2 genotypes did not show significant differences between the UM and the MFC groups. However, the genotype FcγRIIIb-NA2/NA2 was statistically significantly associated with SM patients. Conclusions The data presented in this study suggest that the influence of the FcγRIIa-R/R131, FcγRIIIa-F/F176 and FcγRIIIb-NA2/NA2 genotypes are statistically significantly associated with SM patients. However, the FcγRIIa-H/H13 and FcγRIIIa-V/V176 genotypes have demonstrated a protective effect against SM when compared to UM patients. The impact of the FcyR (IIa, IIIa and IIIb) gene variants and anti-malaria IgG subclasses play an important role in susceptibility ... |
format |
Article in Journal/Newspaper |
author |
Amre Nasr Ahmad Aljada Osama Hamid Hatim A. Elsheikh Emad Masuadi Ahmad Al-Bawab Themer H. Alenazi Amir Abushouk Ayman M. Salah |
author_facet |
Amre Nasr Ahmad Aljada Osama Hamid Hatim A. Elsheikh Emad Masuadi Ahmad Al-Bawab Themer H. Alenazi Amir Abushouk Ayman M. Salah |
author_sort |
Amre Nasr |
title |
Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
title_short |
Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
title_full |
Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
title_fullStr |
Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
title_full_unstemmed |
Significant differences in FcγRIIa, FcγRIIIa and FcγRIIIb genes polymorphism and anti-malarial IgG subclass pattern are associated with severe Plasmodium falciparum malaria in Saudi children |
title_sort |
significant differences in fcγriia, fcγriiia and fcγriiib genes polymorphism and anti-malarial igg subclass pattern are associated with severe plasmodium falciparum malaria in saudi children |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03901-0 https://doaj.org/article/12fa7e86a08c4dfe8c68089edae082c5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-10 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03901-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03901-0 1475-2875 https://doaj.org/article/12fa7e86a08c4dfe8c68089edae082c5 |
op_doi |
https://doi.org/10.1186/s12936-021-03901-0 |
container_title |
Malaria Journal |
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20 |
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1 |
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1766347452027240448 |