Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease.
Chagas disease, caused by Trypanosoma cruzi, is endemic in Latin America and an emerging infectious disease in the US and Europe. We have shown TcG1, TcG2, and TcG4 antigens elicit protective immunity to T. cruzi in mice and dogs. Herein, we investigated antigenicity of the recombinant proteins in h...
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ftdoajarticles:oai:doaj.org/article:12f4ac1bed5041638c9fba560afd533a 2023-05-15T15:16:48+02:00 Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. Shivali Gupta Xianxiu Wan Maria P Zago Valena C Martinez Sellers Trevor S Silva Dadjah Assiah Monisha Dhiman Sonia Nuñez John R Petersen Juan C Vázquez-Chagoyán Jose G Estrada-Franco Nisha Jain Garg 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002018 https://doaj.org/article/12f4ac1bed5041638c9fba560afd533a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3547861?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002018 https://doaj.org/article/12f4ac1bed5041638c9fba560afd533a PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2018 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002018 2022-12-31T02:26:57Z Chagas disease, caused by Trypanosoma cruzi, is endemic in Latin America and an emerging infectious disease in the US and Europe. We have shown TcG1, TcG2, and TcG4 antigens elicit protective immunity to T. cruzi in mice and dogs. Herein, we investigated antigenicity of the recombinant proteins in humans to determine their potential utility for the development of next generation diagnostics for screening of T. cruzi infection and Chagas disease.Sera samples from inhabitants of the endemic areas of Argentina-Bolivia and Mexico-Guatemala were analyzed in 1(st)-phase for anti-T. cruzi antibody response by traditional serology tests; and in 2(nd)-phase for antibody response to the recombinant antigens (individually or mixed) by an ELISA. We noted similar antibody response to candidate antigens in sera samples from inhabitants of Argentina and Mexico (n=175). The IgG antibodies to TcG1, TcG2, and TcG4 (individually) and TcG(mix) were present in 62-71%, 65-78% and 72-82%, and 89-93% of the subjects, respectively, identified to be seropositive by traditional serology. Recombinant TcG1- (93.6%), TcG2- (96%), TcG4- (94.6%) and TcG(mix)- (98%) based ELISA exhibited significantly higher specificity compared to that noted for T. cruzi trypomastigote-based ELISA (77.8%) in diagnosing T. cruzi-infection and avoiding cross-reactivity to Leishmania spp. No significant correlation was noted in the sera levels of antibody response and clinical severity of Chagas disease in seropositive subjects.Three candidate antigens were recognized by antibody response in chagasic patients from two distinct study sites and expressed in diverse strains of the circulating parasites. A multiplex ELISA detecting antibody response to three antigens was highly sensitive and specific in diagnosing T. cruzi infection in humans, suggesting that a diagnostic kit based on TcG1, TcG2 and TcG4 recombinant proteins will be useful in diverse situations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argentina PLoS Neglected Tropical Diseases 7 1 e2018 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Shivali Gupta Xianxiu Wan Maria P Zago Valena C Martinez Sellers Trevor S Silva Dadjah Assiah Monisha Dhiman Sonia Nuñez John R Petersen Juan C Vázquez-Chagoyán Jose G Estrada-Franco Nisha Jain Garg Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chagas disease, caused by Trypanosoma cruzi, is endemic in Latin America and an emerging infectious disease in the US and Europe. We have shown TcG1, TcG2, and TcG4 antigens elicit protective immunity to T. cruzi in mice and dogs. Herein, we investigated antigenicity of the recombinant proteins in humans to determine their potential utility for the development of next generation diagnostics for screening of T. cruzi infection and Chagas disease.Sera samples from inhabitants of the endemic areas of Argentina-Bolivia and Mexico-Guatemala were analyzed in 1(st)-phase for anti-T. cruzi antibody response by traditional serology tests; and in 2(nd)-phase for antibody response to the recombinant antigens (individually or mixed) by an ELISA. We noted similar antibody response to candidate antigens in sera samples from inhabitants of Argentina and Mexico (n=175). The IgG antibodies to TcG1, TcG2, and TcG4 (individually) and TcG(mix) were present in 62-71%, 65-78% and 72-82%, and 89-93% of the subjects, respectively, identified to be seropositive by traditional serology. Recombinant TcG1- (93.6%), TcG2- (96%), TcG4- (94.6%) and TcG(mix)- (98%) based ELISA exhibited significantly higher specificity compared to that noted for T. cruzi trypomastigote-based ELISA (77.8%) in diagnosing T. cruzi-infection and avoiding cross-reactivity to Leishmania spp. No significant correlation was noted in the sera levels of antibody response and clinical severity of Chagas disease in seropositive subjects.Three candidate antigens were recognized by antibody response in chagasic patients from two distinct study sites and expressed in diverse strains of the circulating parasites. A multiplex ELISA detecting antibody response to three antigens was highly sensitive and specific in diagnosing T. cruzi infection in humans, suggesting that a diagnostic kit based on TcG1, TcG2 and TcG4 recombinant proteins will be useful in diverse situations. |
format |
Article in Journal/Newspaper |
author |
Shivali Gupta Xianxiu Wan Maria P Zago Valena C Martinez Sellers Trevor S Silva Dadjah Assiah Monisha Dhiman Sonia Nuñez John R Petersen Juan C Vázquez-Chagoyán Jose G Estrada-Franco Nisha Jain Garg |
author_facet |
Shivali Gupta Xianxiu Wan Maria P Zago Valena C Martinez Sellers Trevor S Silva Dadjah Assiah Monisha Dhiman Sonia Nuñez John R Petersen Juan C Vázquez-Chagoyán Jose G Estrada-Franco Nisha Jain Garg |
author_sort |
Shivali Gupta |
title |
Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
title_short |
Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
title_full |
Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
title_fullStr |
Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
title_full_unstemmed |
Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. |
title_sort |
antigenicity and diagnostic potential of vaccine candidates in human chagas disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002018 https://doaj.org/article/12f4ac1bed5041638c9fba560afd533a |
geographic |
Arctic Argentina |
geographic_facet |
Arctic Argentina |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2018 (2013) |
op_relation |
http://europepmc.org/articles/PMC3547861?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002018 https://doaj.org/article/12f4ac1bed5041638c9fba560afd533a |
op_doi |
https://doi.org/10.1371/journal.pntd.0002018 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
1 |
container_start_page |
e2018 |
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1766347091370573824 |