Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development.
Background The focus on laboratory-based diagnosis of coronavirus disease 2019 (COVID-19) warrants alternative public health tools such as rapid antigen tests. While there are a number of commercially available antigen tests to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), all...
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ftdoajarticles:oai:doaj.org/article:12e088e16bb54e869cac5b25d96be85e 2023-05-15T15:11:01+02:00 Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. Nol Salcedo Ankita Reddy Adam R Gomez Irene Bosch Bobby Brooke Herrera 2022-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010311 https://doaj.org/article/12e088e16bb54e869cac5b25d96be85e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010311 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010311 https://doaj.org/article/12e088e16bb54e869cac5b25d96be85e PLoS Neglected Tropical Diseases, Vol 16, Iss 3, p e0010311 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010311 2022-12-30T21:41:39Z Background The focus on laboratory-based diagnosis of coronavirus disease 2019 (COVID-19) warrants alternative public health tools such as rapid antigen tests. While there are a number of commercially available antigen tests to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), all cross-react with the genetically similar SARS-CoV-1 or require an instrument for results interpretation. Methodology/principal findings We developed and validated rapid antigen tests that use pairs of murine-derived monoclonal antibodies (mAbs), along with gold nanoparticles, to detect SARS-CoV-2 with or without cross-reaction to SARS-CoV-1 and other coronaviruses. In this development, we demonstrate a robust antibody screening methodology for the selection of mAb pairs that can recognize SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. Linear epitope mapping of the mAbs helped elucidate SARS-CoV-2 S and N interactions in lateral flow chromatography. A candidate rapid antigen test for SARS-CoV-2 N was validated using nasal swab specimens that were confirmed positive or negative by quantitative reverse-transcription polymerase chain reaction (RT-PCR). Test results were image-captured using a mobile phone and normalized signal pixel intensities were calculated; signal intensities were inversely correlated to RT-PCR cycle threshold (Ct) value. Conclusion/significance Overall, our results suggest that the rapid antigen test is optimized to detect SARS-CoV-2 N during the acute phase of COVID-19. The rapid antigen tests developed in this study are alternative tools for wide scale public health surveillance of COVID-19. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 3 e0010311 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nol Salcedo Ankita Reddy Adam R Gomez Irene Bosch Bobby Brooke Herrera Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background The focus on laboratory-based diagnosis of coronavirus disease 2019 (COVID-19) warrants alternative public health tools such as rapid antigen tests. While there are a number of commercially available antigen tests to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), all cross-react with the genetically similar SARS-CoV-1 or require an instrument for results interpretation. Methodology/principal findings We developed and validated rapid antigen tests that use pairs of murine-derived monoclonal antibodies (mAbs), along with gold nanoparticles, to detect SARS-CoV-2 with or without cross-reaction to SARS-CoV-1 and other coronaviruses. In this development, we demonstrate a robust antibody screening methodology for the selection of mAb pairs that can recognize SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. Linear epitope mapping of the mAbs helped elucidate SARS-CoV-2 S and N interactions in lateral flow chromatography. A candidate rapid antigen test for SARS-CoV-2 N was validated using nasal swab specimens that were confirmed positive or negative by quantitative reverse-transcription polymerase chain reaction (RT-PCR). Test results were image-captured using a mobile phone and normalized signal pixel intensities were calculated; signal intensities were inversely correlated to RT-PCR cycle threshold (Ct) value. Conclusion/significance Overall, our results suggest that the rapid antigen test is optimized to detect SARS-CoV-2 N during the acute phase of COVID-19. The rapid antigen tests developed in this study are alternative tools for wide scale public health surveillance of COVID-19. |
format |
Article in Journal/Newspaper |
author |
Nol Salcedo Ankita Reddy Adam R Gomez Irene Bosch Bobby Brooke Herrera |
author_facet |
Nol Salcedo Ankita Reddy Adam R Gomez Irene Bosch Bobby Brooke Herrera |
author_sort |
Nol Salcedo |
title |
Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
title_short |
Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
title_full |
Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
title_fullStr |
Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
title_full_unstemmed |
Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development. |
title_sort |
monoclonal antibody pairs against sars-cov-2 for rapid antigen test development. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010311 https://doaj.org/article/12e088e16bb54e869cac5b25d96be85e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 3, p e0010311 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010311 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010311 https://doaj.org/article/12e088e16bb54e869cac5b25d96be85e |
op_doi |
https://doi.org/10.1371/journal.pntd.0010311 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
e0010311 |
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1766341940852293632 |