Genome-Wide Identification and Expression Pattern of ABC Transporters in Razor Clam (Sinonovacula constricta)

• Main Authors: Fan YANG, Zhiping LI, Yinghui DONG, Jianfeng REN
• Format: Article in Journal/Newspaper
• Language: English; Chinese
• Published: Science Press, PR China 2024
• Subjects: sinonovacula constricta; abc transporter; sequence analysis; gene expression pattern; Aquaculture. Fisheries. Angling; SH1-691; Crassostrea gigas
• Online Access: https://doi.org/10.19663/j.issn2095-9869.20220804001; https://doaj.org/article/12ce55ac86fe4845b092c1a8e81bab54

The razor clam (Sinonovacula constricta) is an economically important species and one of China´s four traditional mariculture mollusks. The ABC transporter family is one of the oldest membrane protein families, widespread across prokaryotes and eukaryotes. By utilizing the energy released by ATP hydrolysis, ABC transporters function to transfer amino acids, lipids, antibiotics, and many other substances across membranes, thereby participating in various physiological processes such as nutrition uptake, antigen presentation, drug excretion, and lipid homeostasis in living organisms. ABC transporters can be classified as full transporters, which contain two nucleotide-binding domains (NBDs) and two transmembrane domains (TMDs), a half-transporter (composed of one NBD and one TMD), and a non-transporter (composed of either two NBDs or two TMDs incapable of transporting). The NBD domain is responsible for binding and hydrolyzing ATP, whereas the TMD domain determines substrate specificity. NBD domain sequences are relatively more conserved. Several investigations of heavy metal pollution were conducted in mollusk culture areas, and it was found that the concentrations of some ions exceeded the limits. Previous studies have reported that the multixenobiotic resistance (MXR) mechanism in bivalves is mediated by ABC transporters from the ABCB, ABCC, and ABCG subfamilies, which are important for the cellular efflux of noxious metallic ions. Obtaining a greater understanding of ABC transporters may contribute to the development of a healthier and more scientific method of mollusk culture. Until now, the identification of the ABC transporter family in mollusks has only been systematically performed in three bivalves: Patinopecten yessoensis, Chlamys farreri, and Crassostrea gigas. The analysis and expression pattern of the ABC transporter family of the razor clam have not yet been reported. To systematically study ABC transporters and facilitate an understanding of the evolution and function of ABC transporters in razor ...