Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya.
The composition and structure of microbial communities associated with mosquitoes remain poorly understood despite their important role in host biology and potential to be harnessed as novel strategies for mosquito-borne disease control. We employed MiSeq sequencing of the 16S rRNA gene amplicons to...
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ftdoajarticles:oai:doaj.org/article:12c839a43eac4042a39a6f5fef8f01c3 2023-05-15T15:06:21+02:00 Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. David P Tchouassi Ephantus J Muturi Samwel O Arum Chang-Hyun Kim Christopher J Fields Baldwyn Torto 2019-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007361 https://doaj.org/article/12c839a43eac4042a39a6f5fef8f01c3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007361 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007361 https://doaj.org/article/12c839a43eac4042a39a6f5fef8f01c3 PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007361 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007361 2022-12-31T07:46:37Z The composition and structure of microbial communities associated with mosquitoes remain poorly understood despite their important role in host biology and potential to be harnessed as novel strategies for mosquito-borne disease control. We employed MiSeq sequencing of the 16S rRNA gene amplicons to characterize the bacterial flora of field-collected populations of Aedes mcintoshi and Aedes ochraceus, the primary vectors of Rift Valley fever (RVF) virus in Kenya. Proteobacteria (53.5%), Firmicutes (22.0%) and Actinobacteria (10.0%) were the most abundant bacterial phyla accounting for 85.5% of the total sequences. Non-metric multi-dimensional scaling plots based on Bray-Curtis dissimilarities revealed a clear grouping of the samples by mosquito species, indicating that the two mosquito species harbored distinct microbial communities. Microbial diversity, richness and composition was strongly influenced by the site of mosquito collection and overall, Ae. ochraceus had significantly higher microbial diversity and richness than Ae. mcintoshi. Our findings suggest that host species and site of collection are important determinants of bacterial community composition and diversity in RVF virus vectors and these differences likely contribute to the spatio-temporal transmission dynamics of RVF virus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) PLOS Neglected Tropical Diseases 13 6 e0007361 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 David P Tchouassi Ephantus J Muturi Samwel O Arum Chang-Hyun Kim Christopher J Fields Baldwyn Torto Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The composition and structure of microbial communities associated with mosquitoes remain poorly understood despite their important role in host biology and potential to be harnessed as novel strategies for mosquito-borne disease control. We employed MiSeq sequencing of the 16S rRNA gene amplicons to characterize the bacterial flora of field-collected populations of Aedes mcintoshi and Aedes ochraceus, the primary vectors of Rift Valley fever (RVF) virus in Kenya. Proteobacteria (53.5%), Firmicutes (22.0%) and Actinobacteria (10.0%) were the most abundant bacterial phyla accounting for 85.5% of the total sequences. Non-metric multi-dimensional scaling plots based on Bray-Curtis dissimilarities revealed a clear grouping of the samples by mosquito species, indicating that the two mosquito species harbored distinct microbial communities. Microbial diversity, richness and composition was strongly influenced by the site of mosquito collection and overall, Ae. ochraceus had significantly higher microbial diversity and richness than Ae. mcintoshi. Our findings suggest that host species and site of collection are important determinants of bacterial community composition and diversity in RVF virus vectors and these differences likely contribute to the spatio-temporal transmission dynamics of RVF virus. |
format |
Article in Journal/Newspaper |
author |
David P Tchouassi Ephantus J Muturi Samwel O Arum Chang-Hyun Kim Christopher J Fields Baldwyn Torto |
author_facet |
David P Tchouassi Ephantus J Muturi Samwel O Arum Chang-Hyun Kim Christopher J Fields Baldwyn Torto |
author_sort |
David P Tchouassi |
title |
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
title_short |
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
title_full |
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
title_fullStr |
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
title_full_unstemmed |
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya. |
title_sort |
host species and site of collection shape the microbiota of rift valley fever vectors in kenya. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007361 https://doaj.org/article/12c839a43eac4042a39a6f5fef8f01c3 |
long_lat |
ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Arctic Bray |
geographic_facet |
Arctic Bray |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007361 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007361 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007361 https://doaj.org/article/12c839a43eac4042a39a6f5fef8f01c3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007361 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e0007361 |
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