Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media
Uncultured microorganisms comprise most of the microbial diversity existing on our planet. Despite advances in environmental sequencing and single-cell genomics, in-depth studies about bacterial metabolism and screening of novel bioproducts can only be assessed by culturing microbes in the laborator...
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ftdoajarticles:oai:doaj.org/article:1271ff905c2f40248ef3768ff5aecdd0 2023-05-15T13:46:38+02:00 Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media Andre A. Pulschen Amanda G. Bendia Ashwana D. Fricker Vivian H. Pellizari Douglas Galante Fabio Rodrigues 2017-07-01T00:00:00Z https://doi.org/10.3389/fmicb.2017.01346 https://doaj.org/article/1271ff905c2f40248ef3768ff5aecdd0 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmicb.2017.01346/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.01346 https://doaj.org/article/1271ff905c2f40248ef3768ff5aecdd0 Frontiers in Microbiology, Vol 8 (2017) uncultured Antarctica Solirubrobacterales Thermoleophilia slow-growing bacteria Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.3389/fmicb.2017.01346 2022-12-31T12:47:32Z Uncultured microorganisms comprise most of the microbial diversity existing on our planet. Despite advances in environmental sequencing and single-cell genomics, in-depth studies about bacterial metabolism and screening of novel bioproducts can only be assessed by culturing microbes in the laboratory. Here we report uncultured, or recalcitrant, microorganisms from an Antarctic soil sample, using relatively simple methods: oligotrophic media, extended incubation periods, observation under stereo microscopy, and selection of slow-growing bacteria. We managed to isolate several rare microorganisms belonging to infrequently isolated or recently described genera, for example Lapillicoccus, Flavitalea, Quadrisphaera, Motilibacter, and Polymorphobacter. Additionally, we obtained isolates presenting 16S rRNA sequence similarity ranging from 92.08 to 94.46% with any other known cultured species, including two distinct isolates from the class Thermoleophilia, that although common in Antarctic soils (as identified by metagenomics), was never reported to be isolated from such samples. Our data indicates that simple methods are still useful for cultivating recalcitrant microorganisms, even when dealing with samples from extreme environments. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Microbiology 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
uncultured Antarctica Solirubrobacterales Thermoleophilia slow-growing bacteria Microbiology QR1-502 |
spellingShingle |
uncultured Antarctica Solirubrobacterales Thermoleophilia slow-growing bacteria Microbiology QR1-502 Andre A. Pulschen Amanda G. Bendia Ashwana D. Fricker Vivian H. Pellizari Douglas Galante Fabio Rodrigues Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
topic_facet |
uncultured Antarctica Solirubrobacterales Thermoleophilia slow-growing bacteria Microbiology QR1-502 |
description |
Uncultured microorganisms comprise most of the microbial diversity existing on our planet. Despite advances in environmental sequencing and single-cell genomics, in-depth studies about bacterial metabolism and screening of novel bioproducts can only be assessed by culturing microbes in the laboratory. Here we report uncultured, or recalcitrant, microorganisms from an Antarctic soil sample, using relatively simple methods: oligotrophic media, extended incubation periods, observation under stereo microscopy, and selection of slow-growing bacteria. We managed to isolate several rare microorganisms belonging to infrequently isolated or recently described genera, for example Lapillicoccus, Flavitalea, Quadrisphaera, Motilibacter, and Polymorphobacter. Additionally, we obtained isolates presenting 16S rRNA sequence similarity ranging from 92.08 to 94.46% with any other known cultured species, including two distinct isolates from the class Thermoleophilia, that although common in Antarctic soils (as identified by metagenomics), was never reported to be isolated from such samples. Our data indicates that simple methods are still useful for cultivating recalcitrant microorganisms, even when dealing with samples from extreme environments. |
format |
Article in Journal/Newspaper |
author |
Andre A. Pulschen Amanda G. Bendia Ashwana D. Fricker Vivian H. Pellizari Douglas Galante Fabio Rodrigues |
author_facet |
Andre A. Pulschen Amanda G. Bendia Ashwana D. Fricker Vivian H. Pellizari Douglas Galante Fabio Rodrigues |
author_sort |
Andre A. Pulschen |
title |
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
title_short |
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
title_full |
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
title_fullStr |
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
title_full_unstemmed |
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media |
title_sort |
isolation of uncultured bacteria from antarctica using long incubation periods and low nutritional media |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmicb.2017.01346 https://doaj.org/article/1271ff905c2f40248ef3768ff5aecdd0 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Microbiology, Vol 8 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01346/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.01346 https://doaj.org/article/1271ff905c2f40248ef3768ff5aecdd0 |
op_doi |
https://doi.org/10.3389/fmicb.2017.01346 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
_version_ |
1766244786713395200 |