Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States
In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and...
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ftdoajarticles:oai:doaj.org/article:1271d59992ac436bbc8d4839e270a9c6 2024-09-15T18:31:47+00:00 Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/563293 https://doaj.org/article/1271d59992ac436bbc8d4839e270a9c6 EN eng Wiley http://dx.doi.org/10.1155/2012/563293 https://doaj.org/toc/2090-8113 https://doaj.org/toc/2042-0048 2090-8113 2042-0048 doi:10.1155/2012/563293 https://doaj.org/article/1271d59992ac436bbc8d4839e270a9c6 Veterinary Medicine International, Vol 2012 (2012) Veterinary medicine SF600-1100 article 2012 ftdoajarticles https://doi.org/10.1155/2012/563293 2024-08-05T17:48:44Z In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and serologic tests. The rapid antibody detection tests evaluated were the CervidTB Stat-Pak (Stat-Pak), and the Dual Path Platform VetTB (DPP). Blood was collected from presumably uninfected animals prior to tuberculin injection for the SCT. A total of 1,783 animals were enrolled in the project. Of these, 1,752 (98.3%) were classified as presumably uninfected, based on originating from a captive cervid herd with no history of exposure to TB. Stat-Pak specificity estimates were 92.4% in reindeer, 96.7% in WTD, and 98.3% in elk and were not significantly different from SCT specificity estimates. Using the DPP in series on Stat-Pak antibody-positive samples improved specificity in the three species. Thirty one animals were classified as confirmed infected, based on necropsy and laboratory results, and 27/31 were antibody positive on Stat-Pak for an estimated sensitivity of 87.1%. The study findings indicate that rapid serologic tests used in series are comparable to the SCT and CCT and may have a greater ability to detect TB-infected cervids. Article in Journal/Newspaper Rangifer tarandus Directory of Open Access Journals: DOAJ Articles Veterinary Medicine International 2012 1 7 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Veterinary medicine SF600-1100 |
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Veterinary medicine SF600-1100 Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
topic_facet |
Veterinary medicine SF600-1100 |
description |
In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and serologic tests. The rapid antibody detection tests evaluated were the CervidTB Stat-Pak (Stat-Pak), and the Dual Path Platform VetTB (DPP). Blood was collected from presumably uninfected animals prior to tuberculin injection for the SCT. A total of 1,783 animals were enrolled in the project. Of these, 1,752 (98.3%) were classified as presumably uninfected, based on originating from a captive cervid herd with no history of exposure to TB. Stat-Pak specificity estimates were 92.4% in reindeer, 96.7% in WTD, and 98.3% in elk and were not significantly different from SCT specificity estimates. Using the DPP in series on Stat-Pak antibody-positive samples improved specificity in the three species. Thirty one animals were classified as confirmed infected, based on necropsy and laboratory results, and 27/31 were antibody positive on Stat-Pak for an estimated sensitivity of 87.1%. The study findings indicate that rapid serologic tests used in series are comparable to the SCT and CCT and may have a greater ability to detect TB-infected cervids. |
format |
Article in Journal/Newspaper |
author |
Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall |
author_facet |
Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall |
author_sort |
Jeffrey T. Nelson |
title |
Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
title_short |
Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
title_full |
Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
title_fullStr |
Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
title_full_unstemmed |
Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
title_sort |
evaluation of serodiagnostic assays for mycobacterium bovis infection in elk, white-tailed deer, and reindeer in the united states |
publisher |
Wiley |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/563293 https://doaj.org/article/1271d59992ac436bbc8d4839e270a9c6 |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Veterinary Medicine International, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/563293 https://doaj.org/toc/2090-8113 https://doaj.org/toc/2042-0048 2090-8113 2042-0048 doi:10.1155/2012/563293 https://doaj.org/article/1271d59992ac436bbc8d4839e270a9c6 |
op_doi |
https://doi.org/10.1155/2012/563293 |
container_title |
Veterinary Medicine International |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
7 |
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1810473524930805760 |